Fermentive production of four carbon alcohols

ABSTRACT

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority under 35 U.S.C. §119 from U.S. Provisional Application Ser. No. 60/796,816, filed May 2, 2006 and U.S. Provisional Application Ser. No. 60/871,156, filed Dec. 21, 2006.

FIELD OF THE INVENTION

The invention relates to the field of industrial microbiology and the production of alcohols. More specifically, 2-butanol is produced via industrial fermentation of a recombinant microorganism. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.

BACKGROUND OF THE INVENTION

Butanol is an important industrial chemical, useful as a fuel additive, as a feedstock chemical in the plastics industry, and as a foodgrade extractant in the food and flavor industry. Each year 10 to 12 billion pounds of butanol are produced by petrochemical means and the need for this commodity chemical will likely increase. 2-Butanone, also referred to as methyl ethyl ketone (MEK), is a widely used solvent and is the most important commercially produced ketone, after acetone. It is used as a solvent for paints, resins, and adhesives, as well as a selective extractant and activator of oxidative reactions.

Methods for the chemical synthesis of 2-butanone are known, such as by dehydrogenation of 2-butanol, or in a process where liquid butane is catalytically oxidized giving 2-butanone and acetic acid (Ullmann's Encyclopedia of Industrial Chemistry, 6^(th) edition, 2003, Wiley-VCHVerlag GmbH and Co., Weinheim, Germany, Vol. 5, pp. 727-732). 2-Butanone may also be converted chemically to 2-butanol by hydrogenation (Breen et al., J. or Catalysis 236: 270-281 (2005)). Methods for the chemical synthesis of 2-butanol are known, such as n-butene hydration (Ullmann's Encyclopedia of Industrial Chemistry, 6^(th) edition, 2003, Wiley-VCHVerlag GmbH and Co., Weinheim, Germany, Vol. 5, pp. 716-719). These processes use starting materials derived from petrochemicals and are generally expensive, and are not environmentally friendly. The production of 2-butanone and 2-butanol from plant-derived raw materials would minimize greenhouse gas emissions and would represent an advance in the art.

Methods for producing 2-butanol by biotransformation of other organic chemicals are also known. For example, Stampfer et al. (WO 03/078615) describe the production of secondary alcohols, such as 2-butanol, by the reduction of ketones which is catalyzed by an alcohol dehydrogenase enzyme obtained from Rhodococcus ruber. Similarly, Kojima et al. (EP 0645453) describe a method for preparing secondary alcohols, such as 2-butanol, by reduction of ketones which is catalyzed by a secondary alcohol dehydrogenase enzyme obtained from Candida parapsilosis. Additionally, Kuehnle et al. (EP 1149918) describe a process that produces both 1-butanol and 2-butanol by the oxidation of hydrocarbons by various strains of Rhodococcus ruber. The process favored 1-butanol production with a selectivity of 93.8%.

The production of 2-butanol by certain strains of Lactobacilli is also known (Speranza et. al. J. Agric. Food Chem. (1997) 45:3476-3480). The 2-butanol is produced by the transformation of meso-2,3-butanediol. The production of 2-butanol from acetolactate and acetoin by these Lactobacilli strains was also demonstrated. However, there have been no reports of a recombinant microorganism designed to produce 2-butanol.

There is a need, therefore, for environmentally responsible, cost-effective processes for the production of 2-butanol and 2-butanone. The present invention addresses this need through the discovery of recombinant microbial production hosts expressing 2-butanol and 2-butanone biosynthetic pathways.

SUMMARY OF THE INVENTION

The invention provides a recombinant microorganism having an engineered 2-butanol biosynthetic pathway. Also provided is a recombinant microorganism having an engineered 2-butanone biosynthetic pathway, which is the same as the 2-butanol biosynthetic pathway with omission of the last step. The engineered microorganisms may be used for the commercial production of 2-butanol or 2-butanone. Accordingly, the invention provides a recombinant microbial host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion selected from the group consisting of:

i) pyruvate to alpha-acetolactate;

ii) alpha-acetolactate to acetoin;

iii) acetoin to 2,3-butanediol;

iv) 2,3-butanediol to 2-butanone; and

v) 2-butanone to 2-butanol;

wherein the at least one DNA molecule is heterologous to said microbial host cell and wherein said microbial host cell produces 2-butanol.

In another embodiment the invention provides a recombinant microbial host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion selected from the group consisting of:

i) pyruvate to alpha-acetolactate;

ii) alpha-acetolactate to acetoin;

iii) acetoin to 2,3-butanediol; and

iv) 2,3-butanediol to 2-butanone;

wherein the at least one DNA molecule is heterologous to said microbial host cell and wherein said microbial host cell produces 2-butanone.

In another embodiment the invention provides a method for the production of 2-butanol comprising:

-   -   1) providing a recombinant microbial host cell comprising at         least one DNA molecule encoding a polypeptide that catalyzes a         substrate to product conversion selected from the group         consisting of:         -   i) pyruvate to alpha-acetolactate;         -   ii) alpha-acetolactate to acetoin,         -   iii) acetoin to 2,3-butanediol;         -   iv) 2,3-butanediol to 2-butanone; and         -   v) 2-butanone to 2-butanol;     -   wherein the at least one DNA molecule is heterologous to said         microbial host cell; and     -   2) contacting the host cell of (1) with a fermentable carbon         substrate in a fermentation medium under conditions whereby         2-butanol is produced.

Similarly the invention provides A method for the production of 2-butanone comprising:

-   -   1) providing a recombinant microbial host cell comprising at         least one DNA molecule encoding a polypeptide that catalyzes a         substrate to product conversion selected from the group         consisting of:         -   i) pyruvate to alpha-acetolactate;         -   ii) alpha-acetolactate to acetoin;         -   iii) acetoin to 2,3-butanediol; and         -   iv) 2,3-butanediol to 2-butanone;     -   wherein the at least one DNA molecule is heterologous to said         microbial host cell; and     -   2) contacting the host cell of (1) with a fermentable carbon         substrate in a fermentation medium under conditions whereby         2-butanone is produced.

In another embodiment the invention provides A 2-butanol or 2-butanone containing fermentation product medium produced by the method of the invention.

BRIEF DESCRIPTION OF THE FIGURES, TABLES AND SEQUENCE DESCRIPTIONS

The invention can be more fully understood from the following detailed description, figure, and the accompanying sequence descriptions, which form a part of this application.

FIG. 1 shows four different pathways for biosynthesis of 2-butanone and 2-butanol.

FIG. 2 shows a phylogenetic tree of full length large subunits of diol/glycerol dehydratases, with >95% identical sequences removed (except that all experimentally verified function sequences were retained), and a key listing the identity of each sequence in the tree. Sequences with experimentally determined function as diol or glycerol dehydratases are highlighted in dark or light grey, respectively.

FIG. 3 shows a phylogenetic tree of full length medium subunits of diol/glycerol dehydratases, with >95% identical sequences removed, and a key listing the identity of each sequence in the tree. Sequences with experimentally determined function as diol or glycerol dehydratases are highlighted in dark or light grey, respectively.

FIG. 4 shows a phylogenetic tree of full length small subunits of diol/glycerol dehydratases, with >95% identical sequences removed, and a key listing the identity of each sequence in the tree. Sequences with experimentally determined function as diol or glycerol dehydratases are highlighted in dark or light grey, respectively.

Table 12 is a table of the alpha large subunit Profile HMM for the diol/glycerol dehydratase enzyme. Table 12 is submitted herewith electronically and is incorporated herein by reference.

Table 13 is a table of the beta medium subunit Profile HMM for the diol/glycerol dehydratase enzyme. Table 13 is submitted herewith electronically and is incorporated herein by reference.

Table 14 is a table of the gamma small subunit Profile HMM for the diol/glycerol dehydratase enzyme. Table 14 is submitted herewith electronically and is incorporated herein by reference. The following sequences conform with 37 C.F.R. 1.821-1.825 (“Requirements for Patent Applications Containing Nucleotide Sequences and/or Amino Acid Sequence Disclosures—the Sequence Rules”) and are consistent with World Intellectual Property Organization (WIPO) Standard ST.25 (1998) and the sequence listing requirements of the EPO and PCT (Rules 5.2 and 49.5(a-bis), and Section 208 and Annex C of the Administrative Instructions). The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.

TABLE 1 Summary of Nucleic Acid and Protein SEQ ID Numbers SEQ ID Nucleic SEQ ID Description acid Protein budA, acetolactate decarboxylase from Klebsiella 1 2 pneumoniae ATCC 25955 alsD, acetolactate decarboxylase from Bacillus 80 81 subtilis budA, acetolactate decarboxylase from Klebsiella 82 83 terrigena budB, acetolactate synthase from Klebsiella 3 4 pneumoniae ATCC 25955 alsS, acetolactate synthase from Bacillus subtilis 76 77 budB, acetolactate synthase from Klebsiella 78 79 terrigena budC butanediol dehydrogenase from Klebsiella 5 6 pneumoniae IAM1063 butanediol dehydrogenase from Bacillus cereus 84 85 butanediol dehydrogenase from Bacillus cereus 86 87 butB, butanediol dehydrogenase from Lactococcus 88 89 lactis pddA, butanediol dehydratase alpha subunit from 7 8 Klebsiella oxytoca ATCC 8724 pddB, butanediol dehydratase beta subunit from 9 10 Klebsiella oxytoca ATCC 8724 pddC, butanediol dehydratase gamma subunit from 11 12 Klebsiella oxytoca ATCC 8724 pduC, B12 dependent diol dehydratase large 92 93 subunit from Salmonella typhimurium pduD, B12 dependent diol dehydratase medium 94 95 subunit from Salmonella typhimurium pduE, B12 dependent diol dehydratase small 96 97 subunit from Salmonella typhimurium pduC, B12 dependent diol dehydratase large 98 99 subunit from Lactobacillus collinoides pduD, B12 dependent diol dehydratase medium 100 101 subunit from Lactobacillus collinoides pduE, B12 dependent diol dehydratase small 102 103 subunit from Lactobacillus collinoides pddC, adenosylcobalamin-dependent diol 104 105 dehydratase alpha subunit from Klebsiella pneumoniae pddD, adenosylcobalamin-dependent diol 106 107 dehydratase beta subunit from Klebsiella pneumoniae pddD, adenosylcobalamin-dependent diol 108 109 dehydratase gamma subunit from Klebsiella pneumoniae ddrA, diol dehydratase reactivating factor large 110 111 subunit from Klebsiella oxytoca ddrB, diol dehydratase reactivating factor small 112 113 subunit from Klebsiella oxytoca pduG, diol dehydratase reactivating factor large 114 115 subunit from Salmonella typhimurium pduH, diol dehydratase reactivating factor small 116 117 subunit from Salmonella typhimurium pduG, diol dehydratase reactivating factor large 118 119 subunit from Lactobacillus collinoides pduH, diol dehydratase reactivating factor small 120 121 subunit from Lactobacillus collinoides sadH, butanol dehydrogenase from Rhodococcus 13 14 ruber 219 adhA, butanol dehydrogenase from Pyrococcus 90 91 furiosus chnA, cyclohexanol dehydrogenase from 71 72 Acinteobacter sp. yqhD, butanol dehydrogenase from Escherichia coli 74 75 amine: pyruvate transaminase from Vibrio fluvialis 144 122 (an acetoin aminase) codon opt. amino alcohol kinase from Erwinia carotovora 123 124 subsp. atroseptica amino alcohol O-phosphate lyase from Erwinia 125 126 carotovora subsp. atroseptica budC, acetoin reductase (butanediol 133 134 dehydrogenase) from Klebsiella terrigena (now Raoultella terrigena) glycerol dehydratase alpha subunit from Klebsiella 145 146 pneumoniae glycerol dehydratase beta subunit from Klebsiella 147 148 pneumoniae glycerol dehydratase gamma subunit from 149 150 Klebsiella pneumoniae glycerol dehydratase reactivase large subunit from 151 152 Klebsiella pneumoniae glycerol dehydratase reactivase small subunit from 153 154 Klebsiella pneumoniae

SEQ ID NOs:15-65 are the nucleotide sequences of oligonucleotide PCR, cloning, screening, and sequencing primers used in the Examples.

SEQ ID NO:66 is nucleotide sequence of the deleted region of the yqhD gene in E. coli strain MG1655 ΔyqhCD, described in Example 11.

SEQ ID NO:67 is the nucleotide sequence of a variant of the glucose isomerase promoter 1.6GI.

SEQ ID NO:68 is the nucleotide sequence of the 1.5GI promoter.

SEQ ID NO:69 is the nucleotide sequence of the diol dehydratase operon from Klebsiella oxytoca.

SEQ ID NO:70 is the nucleotide sequence of the diol dehydratase reactivating factor operon from Klebsiella oxytoca.

SEQ ID NO:73 is the nucleotide sequence of pDCQ2, which is described in Example 9.

SEQ ID NOs:127-132 are the nucleotide sequences of additional oligonucleotide PCR and cloning primers used in the Examples.

SEQ ID NO:155 is a codon optimized coding region for the amino alcohol kinase of Erwinia carotovora subsp. atroseptica.

SEQ ID NO:156 is a codon optimized coding region for the amino alcohol O-phosphate lyase of Erwinia carotovora subsp. atroseptica.

SEQ ID NOs:157-163 are the nucleotide sequences of additional oligonucleotide PCR and cloning primers used in the Examples.

SEQ ID NO:275 is the nucleotide sequence of an operon from Erwinia carotovora subsp. atroseptica.

TABLE 2 Additional glycerol and diol dehydratase large, medium and small subunits ^(a)Description ^(b)subunit protein SEQ ID Corresponding subunits from same organism^(c) Glycerol dehydratase alpha subunit from Clostridium L 135 pasteurianum Glycerol dehydratase beta subunit from Clostridium M 136 pasteurianum Glycerol dehydratase gamma subunit from Clostridium S 137 pasteurianum Glycerol dehydratase alpha subunit from Escherichia L 138 blattae Glycerol dehydratase beta subunit from Escherichia M 139 blattae Glycerol dehydratase gamma subunit from Escherichia S 140 blattae Glycerol dehydratase alpha subunit from Citrobacter L 141 freundii Glycerol dehydratase beta subunit from Citrobacter M 142 freundii Glycerol dehydratase gamma subunit from Citrobacter S 143 freundii Diol dehydratase alpha subunit from Lactobacillus brevis L 164 Diol dehydratase beta subunit from Lactobacillus brevis M 165 Diol dehydratase gamma subunit from Lactobacillus brevis S 166 Diol dehydratase alpha subunit from Salmonella enterica L 167 subsp. enterica serovar Choleraesuis str. SC-B67 Diol dehydratase beta subunit from Salmonella enterica M 168 subsp. enterica serovar Choleraesuis str. SC-B67 Diol dehydratase gamma subunit from Salmonella enterica S 169 subsp. enterica serovar Choleraesuis str. SC-B67 Propanediol dehydratase, large subunit from Escherichia L 170 coli E24377A Diol/Glyderol Dehydratase medium subunit from M 171 Escherichia coli E24377A Propanediol dehydratase, small subunit from Escherichia S 172 coli E24377A diol dehydratase large subunit from Shigella sonnei Ss046 L 173 diol dehydratase medium subunit from Shigella sonnei M 174 Ss046 diol dehydratase small subunit from Shigella sonnei Ss046 S 175 Propanediol dehydratase large subunit from Yersinia L 176 bercovieri ATCC 43970 hypothetical protein YberA_01000484 from Yersinia M 177 bercovieri ATCC 43970 Propanediol dehydratase small subunit from Yersinia S 178 bercovieri ATCC 43970 Propanediol dehydratase large subunit from Yersinia L 179 mollaretii ATCC 43969 hypothetical protein YmolA_01001292 from Yersinia M 180 mollaretii ATCC 43969 Propanediol dehydratase small subunit from Yersinia S 181 mollaretii ATCC 43969 Diol dehydratase large subunit from Yersinia enterocolitica L 182 subsp. enterocolitica 8081 Diol dehydratase medium subunit from Yersinia M 183 enterocolitica subsp. enterocolitica 8081 diol dehydratase small subunit from Yersinia enterocolitica S 184 subsp. enterocolitica 8081 Propanediol dehydratase large subunit from Yersinia L 185 intermedia ATCC 29909 diol/glycerol dehydratase medium subunit from Yersinia M 186 intermedia ATCC 29909 Propanediol dehydratase small subunit from Yersinia S 187 intermedia ATCC 29909 glycerol dehydratase large subunit from Listeria welshimeri L 188 serovar 6b str. SLCC5334 propanediol utilization dehydratase medium subunit from M 189 Listeria welshimeri serovar 6b str. SLCC5334 propanediol utilization dehydratase small subunit from S 190 Listeria welshimeri serovar 6b str. SLCC5334 hypothetical protein lin1117 from Listeria innocua L 191 Clip11262 hypothetical protein lin1118 from Listeria innocua M 192 Clip11262 hypothetical protein lin1119 from Listeria innocua S 193 Clip11262 hypothetical protein lmo1153 from Listeria monocytogenes L 194 EGD-e hypothetical protein lmo1154 from Listeria monocytogenes M 195 EGD-e hypothetical protein lmo1155 from Listeria monocytogenes S 196 EGD-e glycerol dehydratase large subunit from Salmonella L 197 enterica subsp. enterica serovar Typhi str. CT18 diol dehydratase medium subunit from Salmonella enterica M 198 subsp. enterica serovar Typhi str. CT18 diol dehydratase small subunit from Salmonella enterica S 199 subsp. enterica serovar Typhi str. CT18 putative glycerol dehydratase large subunit [from L 200 Escherichia coli putative diol dehydratase medium subunit from M 201 Escherichia coli putative diol dehydratase small subunit from Escherichia S 202 coli glycerol dehydratase large subunit from Listeria L 203 monocytogenes str. 4b F2365 propanediol utilization: dehydratase, medium subunit from M 204 Listeria monocytogenes str. 4b F2365 propanediol utilization: dehydratase, small subunit from S 205 Listeria monocytogenes str. 4b F2365 Glycerol dehydratase large subunit pduC, putative from L 206 Streptococcus sanguinis SK36 Propanediol utilization: dehydratase medium subunit, M 207 putative from Streptococcus sanguinis SK36 B12-dependent diol dehydratase small subunit, putative S 208 from Streptococcus sanguinis SK36 DhaB from Escherichia blattae L 209 DhaC from Escherichia blattae M 210 DhaE from Escherichia blattae S 211 coenzyme B12-dependent glycerol dehydrogenase large L 212 subunit from Clostridium perfringens str. 13 coenzyme B12-dependent glycerol dehydrogenase M 213 medium subunit from Clostridium perfringens str. 13 coenzyme B12-dependent glycerol dehydrogenase small S 214 subunit from Clostridium perfringens str. 13 Propanediol dehydratase large subunit from Yersinia L 215 frederiksenii ATCC 33641 hypothetical protein YfreA_01000478 from Yersinia M 216 frederiksenii ATCC 33641] Propanediol dehydratase, small subunit from Yersinia S 217 frederiksenii ATCC 33641 Glycerol dehydratase from Thermoanaerobacter L 218 ethanolicus X514 dehydratase medium subunit from Thermoanaerobacter M 219 ethanolicus X514 dehydratase small subunit from Thermoanaerobacter S 220 ethanolicus X514 glycerol dehydratase large subunit GldC from Lactobacillus L 221 hilgardii _glycerol dehydratase medium subunit GldD from M 222 Lactobacillus hilgardii glycerol dehydratase small subunit GldE from S 223 Lactobacillus hilgardii Glycerol dehydratase from Lactobacillus reuteri JCM 1112 L 224 similar to diol dehydratase gamma subunit from M 225 Lactobacillus reuteri JCM 1112 Propanediol utilization: dehydratase small subunit from S 226 Lactobacillus reuteri JCM 1112 glycerol dehydratase large subunit GldC from Lactobacillus L 227 diolivorans glycerol dehydratase medium subunit GldD from M 228 Lactobacillus diolivorans glycerol dehydratase small subunit GldE from S 229 Lactobacillus diolivorans propanediol dehydratase large subunit from Lactobacillus L 230 reuteri propanediol dehydratase medium subunit from M 231 Lactobacillus reuteri propanediol dehydratase small subunit from Lactobacillus S 232 reuteri glycerol dehydratase large subunit from Mesorhizobium loti L + M 233 MAFF303099 glycerol dehydratase small subunit from Mesorhizobium S 234 loti MAFF303099 Glycerol dehydratase from Mycobacterium vanbaalenii L + M 235 PYR-1 propanediol utilization: dehydratase small subunit from S 236 Mycobacterium vanbaalenii PYR-1 Glycerol dehydratase from Mycobacterium sp. MCS L + M 237 dehydratase small subunit Mycobacterium sp. MCS S 238 Dehydratase large subunit: Dehydratase medium subunit L + M 239 from Mycobacterium flavescens PYR-GCK propanediol utilization: dehydratase, small subunit from S 240 Mycobacterium flavescens PYR-GCK Glycerol dehydratase from Mycobacterium sp. JLS L + M 241 dehydratase small subunit from Mycobacterium sp. JLS S 242 glycerol dehydratase large subunit from Mycobacterium L 243 smegmatis str. MC2 155 dehydratase medium subunit from Mycobacterium M 244 smegmatis str. MC2 155 diol dehydrase gamma subunit from Mycobacterium S 245 smegmatis str. MC2 155 Additional subunits glycerol dehydratase large subunit from Mycobacterium L + M 246 smegmatis str. MC2 155 glycerol dehydratase large subunit from Mycobacterium L + M 247 smegmatis str. MC2 155 coenzyme B12-dependent glycerol dehydrogenase small S 248 subunit from Mycobacterium smegmatis str. MC2 155 coenzyme B12-dependent glycerol dehydrogenase small S 249 subunit from Mycobacterium smegmatis str. MC2 155 diol dehydratase medium subunit from Salmonella enterica M 250 subsp. enterica serovar Paratyphi A str. ATCC 9150 diol dehydratase small subunit from Salmonella enterica S 251 subsp. enterica serovar Paratyphi A str. ATCC 9150 glycerol dehydratase, beta subunit from Clostridium M 252 perfringens SM101 glycerol dehydrase, gamma subunit from Clostridium S 253 perfringens SM101 PduC from Salmonella enterica subsp. enterica serovar L 254 Typhimurium glycerol dehydratase large subunit from Listeria L 255 monocytogenes str. 4b H7858 DhaB from Escherichia blattae L 256 DhaB from uncultured bacterium L 257 DhaB from uncultured bacterium L 258 glycerol dehydratase large subunit GldC from Lactobacillus L 259 collinoides PduD from uncultured bacterium M 260 PduD from uncultured bacterium M 261 DhaC from uncultured bacterium M 262 DhaC from uncultured bacterium M 263 DhaC from uncultured bacterium M 264 coenzyme B12-dependent glycerol dehydratase, medium M 265 subunit from Clostridium perfringens ATCC 13124 unknown M 266 glycerol dehydratase beta subunit from Escherichia blattae M 267 PduE from uncultured bacterium S 268 PduE from uncultured bacterium S 269 dehydratase, small subunit from Listeria monocytogenes S 270 str. ½a F6854 DhaE from uncultured bacterium S 271 DhaE from uncultured bacterium S 272 DhaE from uncultured bacterium S 273 dehydratase small subunit from Listeria monocytogenes S 274 FSL N1-017 ^(a)Description: from the Genbank annotation of the sequence and may not be correct including the glycerol or diol designation, or may not include subunit information. ^(b)Subunit: identified by sequence homology to the large, medium, or small subunit.of the Klebsiella oxytoca enzyme. ^(c)Subunts are listed together that are from the same organism and have annotations as the same enzyme, or have Genbank numbers close together indicating proximity in the genome.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to methods for the production of 2-butanol using recombinant microorganisms. The present invention meets a number of commercial and industrial needs. Butanol is an important industrial commodity chemical with a variety of applications, where its potential as a fuel or fuel additive is particularly significant. Although only a four-carbon alcohol, butanol has an energy content similar to that of gasoline and can be blended with any fossil fuel. Butanol is favored as a fuel or fuel additive as it yields only CO₂ and little or no SO_(X) or NO_(X) when burned in the standard internal combustion engine. Additionally butanol is less corrosive than ethanol, the most preferred fuel additive to date.

In addition to its utility as a biofuel or fuel additive, butanol has the potential of impacting hydrogen distribution problems in the emerging fuel cell industry. Fuel cells today are plagued by safety concerns associated with hydrogen transport and distribution. Butanol can be easily reformed for its hydrogen content and can be distributed through existing gas stations in the purity required for either fuel cells or combustion engines in vehicles.

Finally the present invention produces 2-butanol from plant derived carbon sources, avoiding the negative environmental impact associated with standard petrochemical processes for butanol production.

The present invention also provides recombinant microorganisms and methods for producing 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. 2-Butanone, also known as methyl ethyl ketone (MEK), is useful as a solvent in paints and other coatings. It is also used in the synthetic rubber industry and in the production of paraffin wax.

The following definitions and abbreviations are to be used for the interpretation of the claims and the specification.

The term “invention” or “present invention” as used herein is a non-limiting term and is not intended to refer to any single embodiment of the particular invention but encompasses all possible embodiments as described in the specification and the claims.

The term “2-butanol biosynthetic pathway” refers to the enzyme pathways to produce 2-butanol from pyruvate.

The term “2-butanone biosynthetic pathway” refers to the enzyme pathways to produce 2-butanone from pyruvate.

The term “acetolactate synthase”, also known as “acetohydroxy acid synthase”, refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of two molecules of pyruvic acid to one molecule of alpha-acetolactate. Acetolactate synthase, known as EC 2.2.1.6 [formerly 4.1.3.18] (Enzyme Nomenclature 1992, Academic Press, San Diego) may be dependent on the cofactor thiamin pyrophosphate for its activity. Suitable acetolactate synthase enzymes are available from a number of sources, for example, Bacillus subtilis [GenBank Nos: AAA22222 NCBI (National Center for Biotechnology Information) amino acid sequence (SEQ ID NO:77), L04470 NCBI nucleotide sequence (SEQ ID NO:76)], Klebsiella terrigena [GenBank Nos: AAA25055 (SEQ ID NO:79), L04507 (SEQ ID NO:78)], and Klebsiella pneumoniae [GenBank Nos: AAA25079 (SEQ ID NO:4), M73842 (SEQ ID NO:3)].

The term “acetolactate decarboxylase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of alpha-acetolactate to acetoin. Acetolactate decarboxylases are known as EC 4.1.1.5 and are available, for example, from Bacillus subtilis [GenBank Nos: AAA22223 (SEQ ID NO:81), L04470 (SEQ ID NO:80)], Klebsiella terrigena [GenBank Nos: AAA25054 (SEQ ID NO:83), L04507 (SEQ ID NO:82)] and Klebsiella pneumoniae [GenBank Nos: AAU43774 (SEQ ID NO:2), AY722056 (SEQ ID NO:1)].

The term “acetoin aminase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of acetoin to 3-amino-2-butanol. Acetoin aminase may utilize the cofactor pyridoxal 5′-phosphate or NADH (reduced nicotinamide adenine dinucleotide) or NADPH (reduced nicotinamide adenine dinucleotide phosphate). The resulting product may have (R) or (S) stereochemistry at the 3-position. The pyridoxal phosphate-dependent enzyme may use an amino acid such as alanine or glutamate as the amino donor. The NADH- and NADPH-dependent enzymes may use ammonia as a second substrate. A suitable example of an NADH-dependent acetoin aminase, also known as amino alcohol dehydrogenase, is described by Ito et al. (U.S. Pat. No. 6,432,688). An example of a pyridoxal-dependent acetoin aminase is the amine:pyruvate aminotransferase (also called amine:pyruvate transaminase) described by Shin and Kim (J. Org. Chem. 67:2848-2853 (2002)).

The term “butanol dehydrogenase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the interconversion of 2-butanone and 2-butanol. Butanol dehydrogenases are a subset of a broad family of alcohol dehydrogenases. Butanol dehydrogenase may be NAD- or NADP-dependent. The NAD-dependent enzymes are known as EC 1.1.1.1 and are available, for example, from Rhodococcus ruber [GenBank Nos: CAD36475 (SEQ ID NO:14), AJ491307 (SEQ ID NO:13)]. The NADP-dependent enzymes are known as EC 1.1.1.2 and are available, for example, from Pyrococcus furiosus [GenBank Nos: AAC25556 (SEQ ID NO:91), AF013169 (SEQ ID NO:90)]. Additionally, a butanol dehydrogenase is available from Escherichia coli [GenBank Nos: NP_(—)417-484 (SEQ ID NO:75), NC_(—)000913 (SEQ ID NO:74)] and a cyclohexanol dehydrogenase is available from Acinetobacter sp. [GenBank Nos: AAG10026 (SEQ ID NO:72), AF282240 (SEQ ID NO:71)].

The term “acetoin kinase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of acetoin to phosphoacetoin. Acetoin kinase may utilize ATP (adenosine triphosphate) or phosphoenolpyruvate as the phosphate donor in the reaction. Although there are no reports of enzymes catalyzing this reaction on acetoin, there are enzymes that catalyze the analogous reaction on the similar substrate dihydroxyacetone, for example, enzymes known as EC 2.7.1.29 (Garcia-Alles et al. (2004) Biochemistry 43:13037-13046).

The term “acetoin phosphate aminase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of phosphoacetoin to 3-amino-2-butanol O-phosphate. Acetoin phosphate aminase may use the cofactor pyridoxal 5′-phosphate, NADH or NADPH. The resulting product may have (R) or (S) stereochemistry at the 3-position. The pyridoxal phosphate-dependent enzyme may use an amino acid such as alanine or glutamate. The NADH— and NADPH-dependent enzymes may use ammonia as a second substrate. Although there are no reports of enzymes catalyzing this reaction on phosphoacetoin, there is a pyridoxal phosphate-dependent enzyme that is proposed to carry out the analogous reaction on the similar substrate serinol phosphate (Yasuta et al. (2001) Appl. Environ. Microbiol. 67:4999-5009).

The term “aminobutanol phosphate phospho-lyase”, also called “amino alcohol O-phosphate lyase”, refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of 3-amino-2-butanol O-phosphate to 2-butanone. Aminobutanol phosphate phospho-lyase may utilize the cofactor pyridoxal 5′-phosphate. There are no previous reports of enzymes catalyzing this reaction on aminobutanol phosphate, though there are reports of enzymes that catalyze the analogous reaction on the similar substrate 1-amino-2-propanol phosphate (Jones et al. (1973) Biochem J. 134:167-182). The present invention describes a newly identified aminobutanol phosphate phospho-lyase (SEQ ID NO: 126) from the organism Erwinia carotovora, with the activity demonstrated in Example 15 herein.

The term “aminobutanol kinase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of 3-amino-2-butanol to 3-amino-2-butanol O-phosphate. Aminobutanol kinase may utilize ATP as the phosphate donor. Although there are no reports of enzymes catalyzing this reaction on 3-amino-2-butanol, there are reports of enzymes that catalyze the analogous reaction on the similar substrates ethanolamine and 1-amino-2-propanol (Jones et al., supra). The present invention describes, in Example 14, an amino alcohol kinase of Erwinia carotovora subsp. atroseptica (SEQ ID NO:124). The term “butanediol dehydrogenase” also known as “acetoin reductase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of acetoin to 2,3-butanediol. Butanediol dehydrogenases are a subset of the broad family of alcohol dehydrogenases. Butanediol dehydrogenase enzymes may have specificity for production of (R)- or (S)-stereochemistry in the alcohol product. (S)-specific butanediol dehydrogenases are known as EC 1.1.1.76 and are available, for example, from Klebsiella pneumoniae (GenBank Nos: BBA13085 (SEQ ID NO:6), D86412 (SEQ ID NO:5)). (R)-specific butanediol dehydrogenases are known as EC 1.1.1.4 and are available, for example, from Bacillus cereus [GenBank Nos. NP_(—)830481 (SEQ ID NO:85), NC_(—)004722 (SEQ ID NO:84); AAP07682 (SEQ ID NO:87), AE017000 (SEQ ID NO:86)], and Lactococcus lactis [GenBank Nos. AAK04995 (SEQ ID NO:89), AE006323 (SEQ ID NO:88)].

The term “butanediol dehydratase”, also known as “diol dehydratase” or “propanediol dehydratase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of 2,3-butanediol to 2-butanone. Butanediol dehydratase may utilize the cofactor adenosyl cobalamin (vitamin B12). Adenosyl cobalamin-dependent enzymes are known as EC 4.2.1.28 and are available, for example, from Klebsiella oxytoca [GenBank Nos: BAA08099 (alpha subunit) (SEQ ID NO:8), D45071 (SEQ ID NO:7); BAA08100 (beta subunit) (SEQ ID NO:10), D45071 (SEQ ID NO:9); and BBA08101 (gamma subunit) (SEQ ID NO:12), D45071 (SEQ ID NO:11) (Note all three subunits are required for activity)], and Klebsiella pneumoniae [GenBank Nos: AAC98384 (alpha subunit) (SEQ ID NO:105), AF102064 (SEQ ID NO:104); GenBank Nos: AAC98385 (beta subunit) (SEQ ID NO:107), AF102064 (SEQ ID NO:106), GenBank Nos: AAC98386 (gamma subunit) SEQ ID NO:109), AF102064 (SEQ ID NO:108)]. Other suitable diol dehydratases include, but are not limited to, B12-dependent diol dehydratases available from Salmonella typhimurium [GenBank Nos: AAB84102 (large subunit) (SEQ ID NO:93), AF026270 (SEQ ID NO:92); GenBank Nos: AAB84103 (medium subunit) (SEQ ID NO:95), AF026270 (SEQ ID NO:94); GenBank Nos: AAB84104 (small subunit) (SEQ ID NO:97), AF026270 (SEQ ID NO:96)]; and Lactobacillus collinoides [GenBank Nos: CAC82541 (large subunit) (SEQ ID NO:99), AJ297723 (SEQ ID NO:98); GenBank Nos: CAC82542 (medium subunit) (SEQ ID NO:101); AJ297723 (SEQ ID NO:100); GenBank Nos: CAD01091 (small subunit) (SEQ ID NO:103), AJ297723 (SEQ ID NO:102)]; and enzymes from Lactobacillus brevis (particularly strains CNRZ 734 and CNRZ 735, Speranza et al., supra), and nucleotide sequences that encode the corresponding enzymes. Methods of diol dehydratase gene isolation are well known in the art (e.g., U.S. Pat. No. 5,686,276). Additional diol dehydratases are listed in Table 2.

The term “glycerol dehydratase” refers to a polypeptide (or polypeptides) having an enzyme activity that catalyzes the conversion of glycerol to 3-hydroxypropionaldehyde. Adenosyl cobalamin-dependent glycerol dehydratases are known as EC 4.2.1.30. The glycerol dehydratases of EC 4.2.1.30 are similar to the diol dehydratases in sequence and in having three subunits. The glycerol dehydratases can also be used to convert 2,3-butanediol to 2-butanone. Some examples of glycerol dehydratases of EC 4.2.1.30 include those from Klebsiella pneumoniae (alpha subunit, SEQ ID NO:145, coding region and SEQ ID NO:146, protein; beta subunit, SEQ ID NO:147, coding region and SEQ ID NO:148, protein; and gamma subunit SEQ ID NO:149, coding region and SEQ ID NO:150, protein); from Clostridium pasteurianum [GenBank Nos: 3360389 (alpha subunit, SEQ ID NO:135), 3360390 (beta subunit, SEQ ID NO:136), and 3360391 (gamma subunit, SEQ ID NO:137)]; from Escherichia blattae [GenBank Nos: 60099613 (alpha subunit, SEQ ID NO:138), 57340191 (beta subunit, SEQ ID NO:139), and 57340192 (gamma subunit, SEQ ID NO:140)]; and from Citrobacter freundii [GenBank Nos: 1169287 (alpha subunit, SEQ ID NO:141), 1229154 (beta subunit, SEQ ID NO:142), and 1229155 (gamma subunit, SEQ ID NO:143)]. Note that all three subunits are required for activity. Additional glycerol dehydratases are listed in Table 2.

Diol and glycerol dehydratases may undergo suicide inactivation during catalysis. A reactivating factor protein, also referred to herein as “reactivase”, can be used to reactivate the inactive enzymes (Mori et al., J. Biol. Chem. 272:32034 (1997)). Preferably, the reactivating factor is obtained from the same source as the diol or glycerol dehydratase used. For example, suitable diol dehydratase reactivating factors are available from Klebsiella oxytoca [GenBank Nos: AAC15871 (large subunit) (SEQ ID NO:111), AF017781 (SEQ ID NO:110); GenBank Nos: AAC15872 (small subunit) (SEQ ID NO:113), AF017781 (SEQ ID NO:112)]; Salmonella typhimurium [GenBank Nos: AAB84105 (large subunit) (SEQ ID NO:115), AF026270 (SEQ ID NO:114), GenBank Nos: AAD39008 (small subunit) (SEQ ID NO:117), AF026270 (SEQ ID NO:116)]; and Lactobacillus collinoides [GenBank Nos: CAD01092 (large subunit) (SEQ ID NO:119), AJ297723 (SEQ ID NO:118); GenBank Nos: CAD01093 (small subunit) (SEQ ID NO:121), AJ297723 (SEQ ID NO:120)]. Both the large and small subunits are required for activity. For example, suitable glycerol dehydratase reactivating factors are available from Klebsiella pneumoniae (large subunit, SEQ ID NO:151, coding region and SEQ ID NO:152, protein; and small subunit, SEQ ID NO:153, coding region and SEQ ID NO:154, protein).

The term “a facultative anaerobe” refers to a microorganism that can grow in both aerobic and anaerobic environments.

The term “carbon substrate” or “fermentable carbon substrate” refers to a carbon source capable of being metabolized by host organisms of the present invention and particularly carbon sources selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and one-carbon substrates or mixtures thereof.

The term “gene” refers to a nucleic acid fragment that is capable of being expressed as a specific protein, optionally including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences. “Chimeric gene” refers to any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. “Endogenous gene” refers to a native gene in its natural location in the genome of an organism. A “foreign” or “heterologous” gene refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes. A “transgene” is a gene that has been introduced into the genome by a transformation procedure.

As used herein, an “isolated nucleic acid fragment” or “isolated nucleic acid molecule” or “genetic construct” will be used interchangeably and will mean a polymer of RNA or DNA that is single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases. An isolated nucleic acid fragment in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA.

A nucleic acid fragment is “hybridizable” to another nucleic acid fragment, such as a cDNA, genomic DNA, or RNA molecule, when a single-stranded form of the nucleic acid fragment can anneal to the other nucleic acid fragment under the appropriate conditions of temperature and solution ionic strength. Hybridization and washing conditions are well known and exemplified in Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2^(nd) ed., Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y. (1989), particularly Chapter 11 and Table 11.1 therein (entirely incorporated herein by reference). The conditions of temperature and ionic strength determine the “stringency” of the hybridization. Stringency conditions can be adjusted to screen for moderately similar fragments (such as homologous sequences from distantly related organisms), to highly similar fragments (such as genes that duplicate functional enzymes from closely related organisms). Post-hybridization washes determine stringency conditions. One set of preferred conditions uses a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A more preferred set of stringent conditions uses higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C. Another preferred set of highly stringent conditions uses two final washes in 0.1×SSC, 0.1% SDS at 65° C. An additional set of stringent conditions include hybridization at 0.1×SSC, 0.1% SDS, 65° C. and washes with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS, for example.

Hybridization requires that the two nucleic acids contain complementary sequences, although depending on the stringency of the hybridization, mismatches between bases are possible. The appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art. The greater the degree of similarity or homology between two nucleotide sequences, the greater the value of Tm for hybrids of nucleic acids having those sequences. The relative stability (corresponding to higher Tm) of nucleic acid hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations for calculating Tm have been derived (see Sambrook et al., supra, 9.50-9.51). For hybridizations with shorter nucleic acids, i.e., oligonucleotides, the position of mismatches becomes more important, and the length of the oligonucleotide determines its specificity (see Sambrook et al., supra, 11.7-11.8). In one embodiment the length for a hybridizable nucleic acid is at least about 10 nucleotides. Preferably a minimum length for a hybridizable nucleic acid is at least about 15 nucleotides; more preferably at least about 20 nucleotides; and most preferably the length is at least about 30 nucleotides. Furthermore, the skilled artisan will recognize that the temperature and wash solution salt concentration may be adjusted as necessary according to factors such as length of the probe.

A “substantial portion” of an amino acid or nucleotide sequence is that portion comprising enough of the amino acid sequence of a polypeptide or the nucleotide sequence of a gene to putatively identify that polypeptide or gene, either by manual evaluation of the sequence by one skilled in the art, or by computer-automated sequence comparison and identification using algorithms such as BLAST (Altschul, S. F., et al., J. Mol. Biol., 215:403-410 (1993)). In general, a sequence of ten or more contiguous amino acids or thirty or more nucleotides is necessary in order to putatively identify a polypeptide or nucleic acid sequence as homologous to a known protein or gene. Moreover, with respect to nucleotide sequences, gene specific oligonucleotide probes comprising 20-30 contiguous nucleotides may be used in sequence-dependent methods of gene identification (e.g., Southern hybridization) and isolation (e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition, short oligonucleotides of 12-15 bases may be used as amplification primers in PCR in order to obtain a particular nucleic acid fragment comprising the primers. Accordingly, a “substantial portion” of a nucleotide sequence comprises enough of the sequence to specifically identify and/or isolate a nucleic acid fragment comprising the sequence. The instant specification teaches the complete amino acid and nucleotide sequence encoding particular fungal proteins. The skilled artisan, having the benefit of the sequences as reported herein, may now use all or a substantial portion of the disclosed sequences for purposes known to those skilled in this art. Accordingly, the instant invention comprises the complete sequences as reported in the accompanying Sequence Listing, as well as substantial portions of those sequences as defined above.

The term “complementary” is used to describe the relationship between nucleotide bases that are capable of hybridizing to one another. For example, with respect to DNA, adenosine is complementary to thymine and cytosine is complementary to guanine.

The terms “homology” and “homologous” are used interchangeably herein. They refer to nucleic acid fragments wherein changes in one or more nucleotide bases do not affect the ability of the nucleic acid fragment to mediate gene expression or produce a certain phenotype. These terms also refer to modifications of the nucleic acid fragments of the instant invention such as deletion or insertion of one or more nucleotides that do not substantially alter the functional properties of the resulting nucleic acid fragment relative to the initial, unmodified fragment. It is therefore understood, as those skilled in the art will appreciate, that the invention encompasses more than the specific exemplary sequences.

Moreover, the skilled artisan recognizes that homologous nucleic acid sequences encompassed by this invention are also defined by their ability to hybridize, under moderately stringent conditions (e.g., 0.5×SSC, 0.1% SDS, 60° C.) with the sequences exemplified herein, or to any portion of the nucleotide sequences disclosed herein and which are functionally equivalent to any of the nucleic acid sequences disclosed herein.

“Codon degeneracy” refers to the nature in the genetic code permitting variation of the nucleotide sequence without effecting the amino acid sequence of an encoded polypeptide. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a gene for improved expression in a host cell, it is desirable to design the gene such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.

The term “percent identity”, as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as determined by comparing the sequences. In the art, “identity” also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. “Identity” and “similarity” can be readily calculated by known methods, including but not limited to those described in: 1.) Computational Molecular Biology (Lesk, A. M., Ed.) Oxford University: NY (1988); 2.) Biocomputing: Informatics and Genome Projects (Smith, D. W., Ed.) Academic: NY (1993); 3.) Computer Analysis of Sequence Data, Part I (Griffin, A. M., and Griffin, H. G., Eds.) Humania: NJ (1994); 4.) Sequence Analysis in Molecular Biology (von Heinje, G., Ed.) Academic (1987); and 5.) Sequence Analysis Primer (Gribskov, M. and Devereux, J., Eds.) Stockton: NY (1991).

Preferred methods to determine identity are designed to give the best match between the sequences tested. Methods to determine identity and similarity are codified in publicly available computer programs. Sequence alignments and percent identity calculations may be performed using the MegAlign™ program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences is performed using the “Clustal method of alignment” which encompasses several varieties of the algorithm including the “Clustal V method of alignment” corresponding to the alignment method labeled Clustal V (described by Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D. G. et al., Comput. Appl. Biosci., 8:189-191 (1992)) and found in the MegAlign™ program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.). For multiple alignments, the default values correspond to GAP PENALTY=10 and GAP LENGTH PENALTY=10. Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal method are KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4. After alignment of the sequences using the Clustal V program, it is possible to obtain a “percent identity” by viewing the “sequence distances” table in the same program. Additionally the “Clustal W method of alignment” is available and corresponds to the alignment method labeled Clustal W (described by Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D. G. et al., Comput. Appl. Biosci. 8:189-191 (1992)) and found in the MegAlign™ v6.1 program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.). Default parameters for multiple alignment (GAP PENALTY=10, GAP LENGTH PENALTY=0.2, Delay Divergen Seqs(%)=30, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB). After alignment of the sequences using the Clustal W program, it is possible to obtain a “percent identity” by viewing the “sequence distances” table in the same program.

It is well understood by one skilled in the art that many levels of sequence identity are useful in identifying polypeptides, from other species, wherein such polypeptides have the same or similar function or activity. Useful examples of percent identities include, but are not limited to: 24%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%, or any integer percentage from 24% to 100% may be useful in describing the present invention, such as 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%. Suitable nucleic acid fragments not only have the above homologies but typically encode a polypeptide having at least 50 amino acids, preferably at least 100 amino acids, more preferably at least 150 amino acids, still more preferably at least 200 amino acids, and most preferably at least 250 amino acids.

The term “sequence analysis software” refers to any computer algorithm or software program that is useful for the analysis of nucleotide or amino acid sequences. “Sequence analysis software” may be commercially available or independently developed. Typical sequence analysis software will include, but is not limited to: 1.) the GCG suite of programs (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, Wis.); 2.) BLASTP, BLASTN, BLASTX (Altschul et al., J. Mol. Biol., 215:403-410 (1990)); 3.) DNASTAR (DNASTAR, Inc. Madison, Wis.); 4.) Sequencher (Gene Codes Corporation, Ann Arbor, Mich.); and 5.) the FASTA program incorporating the Smith-Waterman algorithm (W. R. Pearson, Comput. Methods Genome Res., [Proc. Int. Symp.] (1994), Meeting Date 1992, 111-20. Editor(s): Suhai, Sandor. Plenum: New York, N.Y.). Within the context of this application it will be understood that where sequence analysis software is used for analysis, that the results of the analysis will be based on the “default values” of the program referenced, unless otherwise specified. As used herein “default values” will mean any set of values or parameters that originally load with the software when first initialized.

As used herein the term “coding sequence” or “CDS” refers to a DNA sequence that codes for a specific amino acid sequence. “Suitable regulatory sequences” refer to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include promoters, translation leader sequences, introns, polyadenylation recognition sequences, RNA processing site, effector binding site and stem-loop structure.

The term “promoter” refers to a DNA sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3′ to a promoter sequence. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental or physiological conditions. Promoters which cause a gene to be expressed in most cell types at most times are commonly referred to as “constitutive promoters”. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of different lengths may have identical promoter activity.

The term “operably linked” refers to the association of nucleic acid sequences on a single nucleic acid fragment so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of effecting the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation.

The term “expression”, as used herein, refers to the transcription and stable accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid fragment of the invention. Expression may also refer to translation of mRNA into a polypeptide.

As used herein the term “transformation” refers to the transfer of a nucleic acid fragment into a host organism, resulting in genetically stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as “transgenic” or “recombinant” or “transformed” organisms.

The terms “plasmid” and “vector” refer to an extra chromosomal element often carrying genes which are not part of the central metabolism of the cell, and usually in the form of circular double-stranded DNA fragments. Such elements may be autonomously replicating sequences, genome integrating sequences, phage or nucleotide sequences, linear or circular, of a single- or double-stranded DNA or RNA, derived from any source, in which a number of nucleotide sequences have been joined or recombined into a unique construction which is capable of introducing a promoter fragment and DNA sequence for a selected gene product along with appropriate 3′ untranslated sequence into a cell. “Transformation vector” refers to a specific vector containing a foreign gene and having elements in addition to the foreign gene that facilitates transformation of a particular host cell.

As used herein the term “codon degeneracy” refers to the nature in the genetic code permitting variation of the nucleotide sequence without affecting the amino acid sequence of an encoded polypeptide. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a gene for improved expression in a host cell, it is desirable to design the gene such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.

The term “codon-optimized” as it refers to genes or coding regions of nucleic acid molecules for transformation of various hosts, refers to the alteration of codons in the gene or coding regions of the nucleic acid molecules to reflect the typical codon usage of the host organism without altering the polypeptide encoded by the DNA.

The term “fermentation product medium” refers to a medium in which fermentation has occurred such that product is present in the medium.

Standard recombinant DNA and molecular cloning techniques used here are well known in the art and are described by Sambrook, J., Fritsch, E. F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989) (hereinafter “Maniatis”); and by Silhavy, T. J., Bennan, M. L. and Enquist, L. W., Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1984); and by Ausubel, F. M. et al., Current Protocols in Molecular Biology, published by Greene Publishing Assoc. and Wiley-Interscience (1987).

The 2-Butanol and 2-Butanone Biosynthetic Pathways

Carbohydrate utilizing microorganisms employ the Embden-Meyerhof-Parnas (EMP) pathway, the Entner-Doudoroff pathway and the pentose phosphate cycle as the central, metabolic routes to provide energy and cellular precursors for growth and maintenance. These pathways have in common the intermediate glyceraldehyde-3-phosphate, and, ultimately, pyruvate is formed directly or in combination with the EMP pathway. The combined reactions of sugar conversion to pyruvate produce energy (e.g. adenosine-5′-triphosphate, ATP) and reducing equivalents (e.g. reduced nicotinamide adenine dinucleotide, NADH, and reduced nicotinamide adenine dinucleotide phosphate, NADPH). NADH and NADPH must be recycled to their oxidized forms (NAD⁺ and NADP⁺, respectively). In the presence of inorganic electron acceptors (e.g. O₂, NO₃ ⁻ and SO₄ ²⁻), the reducing equivalents may be used to augment the energy pool; alternatively, a reduced carbon by-product may be formed.

The invention enables the production of 2-butanone or 2-butanol from carbohydrate sources with recombinant microorganisms by providing a complete biosynthetic pathway from pyruvate to 2-butanone or 2-butanol. Three additional pathways are described. Although 2-butanol is not known to be the major product of any bacterial fermentation, there are a number of possible pathways for the production of 2-butanol via known biochemical reaction types. These pathways are shown in FIG. 1. The letters and roman numerals cited below correspond to the letters and roman numerals in FIG. 1, which are used to depict the conversion steps and products, respectively. As described below, 2-butanone is an intermediate in all of these 2-butanol biosynthetic pathways.

All of the pathways begin with the initial reaction of two pyruvate molecules to yield alpha-acetolactate (I), shown as the substrate to product conversion (a) in FIG. 1. From alpha-acetolactate, there are 4 possible pathways to 2-butanone (V), referred to herein as 2-butanone biosynthetic pathways:

Pathway 1) I--->II--->III--->IV--->V (substrate to product conversions b, c, d, e);

-   -   2) I--->II--->VII--->IV--->V (substrate to product conversions         b, g, h, e)     -   3) I--->II--->VIII--->V (substrate to product conversions b, i,         j): This is the pathway of the present invention.     -   4) I--->IX--->X--->V (substrate to product conversions k, l, m)         The 2-butanol biosynthetic pathways conclude with the conversion         of 2-butanone (V) to 2-butanol (VI). A detailed discussion of         the substrate to product conversions in each pathway is given         below.

Pathway 1: (a) pyruvate to alpha-acetolactate

The initial step in pathway 1 is the conversion of two molecules of pyruvate to one molecule of alpha-acetolactate (compound I in FIG. 1) and one molecule of carbon dioxide catalyzed by a thiamin pyrophosphate-dependent enzyme. Enzymes catalyzing this substrate to product conversion (generally called either acetolactate synthase or acetohydroxy acid synthase; EC 2.2.1.6 [switched from 4.1.3.18 in 2002]) are well-known, and they participate in the biosynthetic pathway for the proteinogenic amino acids leucine and valine, as well as in the pathway for fermentative production of 2,3-butanediol and acetoin of a number of organisms.

The skilled person will appreciate that polypeptides having acetolactate synthase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. Some example of suitable acetolactate synthase enzymes are available from a number of sources, for example, Bacillus subtilis [GenBank Nos: AAA22222 NCBI (National Center for Biotechnology Information) amino acid sequence (SEQ ID NO:77), L04470 NCBI nucleotide sequence (SEQ ID NO:76)], Klebsiella terrigena [GenBank Nos: AAA25055 (SEQ ID NO:79), L04507 (SEQ ID NO:78)], and Klebsiella pneumoniae [GenBank Nos: AAA25079 (SEQ ID NO:4), M73842 (SEQ ID NO:3)]. Preferred acetolactate synthase enzymes are those that have at least 80%-85% identity to SEQ ID NO's 4, 77, and 79, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

(b) alpha-acetolactate to acetoin

Alpha-acetolactate (I) is converted to acetoin (II) by the action of an enzyme such as acetolactate decarboxylase (EC 4.1.1.5). Like acetolactate synthase, this enzyme is thiamin pyrophosphate-dependent and is also involved in the production of 2,3-butanediol and acetoin by a number of organisms. The enzymes from different sources vary quite widely in size (25-50 kilodaltons), oligomerization (dimer-hexamer), localization (intracellular of extracellular), and allosteric regulation (for example, activation by branched-chain amino acids). For the purpose of the present invention, an intracellular location is preferable to extracellular, but other variations are generally acceptable.

The skilled person will appreciate that polypeptides having acetolactate decarboxylase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. Some example of suitable acetolactate decarboxylase enzymes are available from a number of sources, for example, Bacillus subtilis [GenBank Nos: AAA22223 (SEQ ID NO:81), L04470 (SEQ ID NO:80)], Klebsiella terrigena [GenBank Nos: AAA25054 (SEQ ID NO:83), L04507 (SEQ ID NO:82)] and Klebsiella pneumoniae [GenBank Nos: AAU43774 (SEQ ID NO:2), AY722056 (SEQ ID NO:1)].

Preferred acetolactate decarboxylase enzymes are those that have at least 80%-85% identity to SEQ ID NO's 2, 81 and 83, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

(c) acetoin to 3-amino-2-butanol

There are two known types of biochemical reactions that could effect the substrate to product conversion of acetoin (II) to 3-amino-2-butanol (III), specifically, pyridoxal phosphate-dependent transamination utilizing an accessory amino donor and direct reductive amination with ammonia. In the latter case, the reducing equivalents are supplied in the form of a reduced nicotinamide cofactor (either NADH or NADPH). An example of an NADH-dependent enzyme catalyzing this reaction with acetoin as a substrate is reported by Ito et al. (U.S. Pat. No. 6,432,688). Any stereospecificity of this enzyme has not been assessed. An example of a pyridoxal phosphate-dependent transaminase that catalyzes the conversion of acetoin to 3-amino-2-butanol has been reported by Shin and Kim (supra). This enzyme was shown in Example 13 herein to convert both the (R) isomer of acetoin to the (2R,3S) isomer of 3-amino-2-butanol and the (S) isomer of acetoin to the (2S,3S) isomer of 3-amino-2-butanol. Either type of enzyme (i.e., transaminase or reductive aminase) is considered to be an acetoin aminase and may be utilized in the production of 2-butanol. Other enzymes in this group may have different stereospecificities.

The skilled person will appreciate that polypeptides having acetoin aminase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. One example of this activity has is described herein and is identified as SEQ ID NO:122. Accordingly preferred acetoin aminase enzymes are those that have at least 80%-85% identity to SEQ ID NO:122, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

(d) 3-amino-2-butanol to 3-amino-2-butanol O-phosphate

There are no enzymes known in the art that catalyze the substrate to product conversion of 3-amino-2-butanol (III) to 3-amino-2-butanol phosphate (IV). However, a few Pseudomonas and Erwinia species have been shown to express an ATP-dependent ethanolamine kinase (EC 2.7.1.82) which allows them to utilize ethanolamine or 1-amino-2-propanol as a nitrogen source (Jones et al. (1973) Biochem. J. 134:167-182). It is likely that this enzyme also has activity towards 3-amino-2-butanol or could be engineered to do so, thereby providing an aminobutanol kinase.

The present invention describes in Example 14 a gene of Erwinia carotovora subsp. atroseptica (SEQ ID NO:123) that encodes a protein (SEQ ID NO:24) that is identified as an amino alcohol kinase. This enzyme may be used to convert 3-amino-2-butanol to 3-amino-2-butanol O-phosphate.

The skilled person will appreciate that polypeptides having aminobutanol kinase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. One example of this activity has is described herein and is identified as SEQ ID NO:124. Accordingly preferred aminobutanol kinase enzymes are those that have at least 80%-85% identity to SEQ ID NO:124, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

(e) 3-amino-2-butanol phosphate to 2-butanone

Although there are no enzymes reported to catalyze the substrate to product conversion of 3-amino-2-butanol phosphate (IV) to 2-butanone (V), the substrate is very similar to those utilized by the pyridoxal phosphate-dependent phosphoethanolamine phospho-lyase enzyme, which has been found in a small number of Pseudomonas and Erwinia species. These enzymes have activity towards phosphoethanolamine and both enantiomers of 2-phospho-1-aminopropane (Jones et al. (1973) Biochem. J. 134:167-182), and may also have activity towards 3-amino-2-butanol O-phosphate. The present invention describes a gene of Erwinia carotovora subsp. atroseptica (SEQ ID NO:125) that encodes a protein (SEQ ID NO:126) with homology to class III aminotransferases. Example 15 demonstrates that this enzyme is active on both aminopropanol phosphate and aminobutanol phosphate substrates. The newly identified and characterized enzyme was able to catalyze the conversion of a mixture of (R)-3-amino-(S)-2-butanol and (S)-3-amino-(R)-2-butanol O-phosphate, and a mixture of (R)-3-amino-(R)-2-butanol and (S)-3-amino-(S)-2-butanol O-phosphate to 2-butanone. The newly identified and characterized enzyme was also able to catalyze the conversion of both (R) and (S)-2-amino-1-propanol phosphate to propanone, with a preference for (S)-2-amino-1-propanol phosphate. The highest activity was observed with the proposed natural substrate DL-1-amino-2-propanol phosphate, which was converted to propionaldehyde.

The skilled person will appreciate that polypeptides having aminobutanol phosphate phospho-lyase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. One example of a suitable aminobutanol phosphate phospho-lyase enzyme is described herein as SEQ ID NO: 126. Accordingly preferred aminobutanol phosphate phospho-lyase enzymes are those that have at least 80%-85% identity to SEQ ID NO's 126, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

(f) 2-butanone to 2-butanol

The final step in all pathways to produce 2-butanol from pyruvic acid is the reduction of 2-butanone (V) to 2-butanol (VI). This substrate to product conversion is catalyzed by some members of the broad class of alcohol dehydrogenases (types utilizing either NADH or NADPH as a source of hydride, depending on the enzyme) that may be called butanol dehydrogenases. Enzymes of each type that catalyze the reduction of 2-butanone are well known, as described above in the definition for butanol dehydrogenase.

The skilled person will appreciate that polypeptides having butanol dehydrogenase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. Some example of suitable butanol dehydrogenase enzymes are available from a number of sources, for example, Rhodococcus ruber [GenBank Nos: CAD36475 (SEQ ID NO:14), AJ491307 (SEQ ID NO:13)]. The NADP-dependent enzymes are known as EC 1.1.1.2 and are available, for example, from Pyrococcus furiosus [GenBank Nos: AAC25556 (SEQ ID NO:91), AF013169 (SEQ ID NO:90)]. Additionally, a butanol dehydrogenase is available from Escherichia coli [GenBank Nos:NP_(—)417-484 (SEQ ID NO:75), NC_(—)000913 (SEQ ID NO:74)] and a cyclohexanol dehydrogenase is available from Acinetobacter sp. [GenBank Nos: AAG10026 (SEQ ID NO:72), AF282240 (SEQ ID NO:71)]. Preferred butanol dehydrogenase enzymes are those that have at least 80%-85% identity to SEQ ID NO's 14, 91, 75, and 72, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

Pathway 2: (a) pyruvate to alpha-acetolactate

This substrate to product conversion is the same as described above for Pathway 1.

(b) alpha-acetolactate to acetoin

This substrate to product conversion is the same as described above for Pathway 1.

(g) acetoin to phosphoacetoin

Although enzymes that catalyze the substrate to product conversion of acetoin (II) to phosphoacetoin (VII) have not been described, the structure of the substrate acetoin is very similar to that of dihydroxyacetone, and therefore acetoin may be an acceptable substrate for dihydroxyacetone kinase (EC 2.7.1.29), an enzyme which catalyzes phosphorylation of dihydroxyacetone. Protein engineering techniques for the alteration of substrate specificity of enzymes are well known (Antikainen and Martin (2005) Bioorg. Med. Chem. 13:2701-2716) and may be used to generate an enzyme with the required specificity. In this conversion, the phosphate moiety may be supplied by any high energy biological phosphate donor, with the common substrates being phosphoenolpyruvate (as in the E. coli dihydroxyacetone kinase) and ATP (as in the Citrobacter freundii dihydroxyacetone kinase) (Garcia-Alles et al. (2004) Biochemistry 43:13037-13045).

(h) phosphoacetoin to 3-amino-2-butanol O-phosphate

Although enzymes that catalyze the substrate to product conversion of phosphoacetoin (VII) to 3-amino-2-butanol O-phosphate (IV) have not been described, the structure of the substrate is very similar to that of dihydroxyacetone phosphate, a substrate for the proposed serinol phosphate aminotransferase encoded by the 5′ portion of the rtxA gene in some species of Bradyrhizobium (Yasuta et al., supra). Thus a serinol phosphate aminotransferase may be functional in this step.

(e) 3-amino-2-butanol O-phosphate to 2-butanone

This substrate to product conversion is the same as described above for Pathway 1.

(f) 2-butanone to 2-butanol

This substrate to product conversion is the same as described above for Pathway 1.

Pathway 3: (a) pyruvate to alpha-acetolactate

This substrate to product conversion is the same as described above for Pathway 1.

(b) alpha-acetolactate to acetoin

This substrate to product conversion is the same as described above for Pathway 1.

(i) acetoin to 2,3-butanediol

The substrate to product conversion of acetoin (II) to 2,3-butanediol (Vil) may be catalyzed by a butanediol dehydrogenase that may either utilize NADH or NADPH as the source of reducing equivalents when carrying out reductions. Enzymes with activity towards acetoin participate in the pathway for production of 2,3-butanediol in organisms that produce that compound. The reported enzymes (e.g., BudC from Klebsiella pneumoniae (Ui et al. (2004) Letters in Applied Microbiology 39:533-537) generally utilize NADH. Either cofactor is acceptable for use in the production of 2-butanol by this pathway.

The skilled person will appreciate that polypeptides having butanediol dehydrogenase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. Some example of suitable butanediol dehydrogenase enzymes are available from a number of sources, for example, Klebsiella pneumoniae (GenBank Nos: BBA13085 (SEQ ID NO:6), D86412 (SEQ ID NO:5)). (R)-specific butanediol dehydrogenases are known as EC 1.1.1.4 and are available, for example, from Bacillus cereus [GenBank Nos. NP_(—)830481 (SEQ ID NO:85), NC_(—)004722 (SEQ ID NO:84); AAP07682 (SEQ ID NO:87), AE017000 (SEQ ID NO:86)], and Lactococcus lactis [GenBank Nos. AAK04995 (SEQ ID NO:89), AE006323 (SEQ ID NO:88)]. Preferred butanediol dehydrogenases enzymes are those that have at least 80%-85% identity to SEQ ID NO's 6, 85, 87, and 89, where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

(j) 2,3-butanediol to 2-butanone

The substrate to product conversion of 2,3-butanediol (VIII) to 2-butanone (V) may be catalyzed by diol dehydratase enzymes (EC 4.2.1.28) and glycerol dehydratase enzymes (EC 4.2.1.30). The best characterized diol dehydratase is the coenzyme B12-dependent Klebsiella oxytoca enzyme, but similar enzymes are found in a number of enteric bacteria. The K. oxytoca enzyme has been shown to accept meso-2,3-butanediol as a substrate (Bachovchin et al. (1977) Biochemistry 16:1082-1092), producing the desired product 2-butanone. Example 17 demonstrates that the Klebsiella pneumoniae glycerol dehydratase was able to convert meso-2,3-butanediol to 2-butanone. The three subunit of the Klebsiella pneumoniae glycerol dehydratase (alpha: SEQ ID NO:145 (coding region) and 146 (protein); beta: SEQ ID NO: 147 (coding region) and 148 (protein); and gamma: SEQ ID NO: 149 (coding region) and 150 (protein)) were expressed in conjunction with the two subunits of the Klebsiella pneumoniae glycerol dehydratase reactivase (large subunit, SEQ ID NO: 151 (coding region) and 152 (protein); and small subunit, SEQ ID NO: 153 (coding region) and 154 (protein)) to provide activity.

There are also reports in the literature of a B12-independent diol dehydratase from Clostridium glycolicum (Hartmanis et al. (1986) Arch. Biochem. Biophys. 245:144-152). This enzyme has activity towards 2,3-butanediol, although this activity is less than 1% of the activity towards ethanediol, but the enzyme may be engineered to improve that activity. A better-characterized B12-independent dehydratase is the glycerol dehydratase from Clostridium butyricum (O'Brien et al. (2004) Biochemistry 43:4635-4645), which has high activity towards 1,2-propanediol as well as glycerol. This enzyme uses S-adenosylmethionine as a source of adenosyl radical. There are no reports of activity towards 2,3-butanediol, but such activity, if not already present, may possibly be engineered.

The skilled person will appreciate that polypeptides having butanediol dehydrogenase activity isolated from a variety of sources will be useful in the present invention independent of sequence homology. As noted above a variety of diol and glycerol dehydratases have been described in the literature and will be suitable for use in the present invention. Accordingly, in one aspect of the invention preferred diol and glycerol dehydratase enzymes are those that have at least 80%-85% identity to enzymes having the large, medium and small subunits, respectively of the sequences listed below:

a) SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12;

b) SEQ ID NO:93, SEQ ID NO:95, and SEQ ID NO:97;

c) SEQ ID NO:99, SEQ ID NO:101, and SEQ ID NO:103;

d) SEQ ID NO:105, SEQ ID NO:107, and SEQ ID NO:109;

e) SEQ ID NO:135, SEQ ID NO:136, and SEQ ID NO:137;

f) SEQ ID NO:138, SEQ ID NO:139, and SEQ ID NO:140;

g) SEQ ID NO:146, SEQ ID NO:148, and SEQ ID NO:150;

h) SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:143; and

i) SEQ ID NO:164, SEQ ID NO:165, and SEQ ID NO:166.

where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

Similarly preferred diol and glycerol dehydratase enzymes are those that have at least 80%-85% identity to enzymes having the large, medium and small subunits, respectively of the sequences listed below: Large subunit: SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164; Medium subunit: SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; Small subunit: SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; where at least 85%-90% identity is more preferred and where at least 95% identity based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix, is most preferred.

Additional diol and glycerol dehydratase enzymes that may be used in the biosynthetic pathway 3 of the present invention were identified through a bioinformatics structure/function analysis that is described below and in Example 18.

(f) 2-butanone to 2-butanol

This substrate to product conversion is the same as described above for Pathway 1.

Diol and Glycerol Dehydratases for Biosynthetic Pathway 3

Any enzyme that is a diol or glycerol dehydratase may be used in the present invention for the conversion of 2,3-butanediol to 2-butanone. A structure/function relationship for diol and glycerol dehydratases in the enzyme classes EC 4.2.1.28 and EC 4.2.1.30, respectively, was established herein in Example 18. The function is provided by experimental data and the structure is provided by bioinformatics analysis. Eight diol and glycerol dehydratase enzymes with activities that have been experimentally demonstrated were analyzed. In this group (listed in Table 10), the Klebsiella oxytoca diol dehydratase and the Klebsiella pneumoniae glycerol dehydratase enzymes were both shown to convert 2,3-butanediol to 2-butanone (Bachovchin et al. (1977) Biochemistry 16:1082-1092 and Example 17 herein, respectively), while the activities of the additional six enzymes were demonstrated using their natural substrates (references given in Table 10). This set of eight diol and glycerol dehydratases was analyzed using the hmmsearch algorithm of the HMMER software package (Janelia Farm Research Campus, Ashburn, Va.). The Z parameter of the hmmsearch algorithm was set to 1 billion. The output of the HMMER analysis using a set of protein sequences is a Profile Hidden Markov Model (Profile HMM). The theory behind Profile HMMs is described in Durbin et al., Biological sequence analysis: probabilistic models of proteins and nucleic acids, Cambridge University Press, 1998; Krogh et al., 1994; J. Mol. Biol. 235:1501-1531, incorporated herein by reference) that characterizes the set of proteins based on the probability of each amino acid occurring at each position in the alignment of the proteins of the set.

Since the eight diol and glycerol dehydratases (diol/glycerol dehydratases) with experimentally verified function that were used for the analysis each have three subunits (large or alpha, medium or beta, and small or gamma), a separate Profile HMM was prepared for each subunit. The large subunit Profile HMM (Table 12) was built using proteins with SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164 that are described in Tables 1 and 2. The medium subunit Profile HMM (Table 13) was built using proteins with SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165 that are described in Tables 1 and 2. The small subunit Profile HMM (Table 14) was built using proteins with SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166 that are described in Tables 1 and 2. References that provide the functional assay data are given in Table 10. The Profile HMM prepared for the large subunit gives a structural characterization for the functional large subunit of diol/glycerol dehydratases. Similarly the Profile HMMs for the medium and small subunits give structural characterizations for the functional medium and small subunits, respectively, of diol/glycerol dehydratases. Therefore any protein that has a significant match to either the large, medium, or small subunit Profile HMM is directly linked to the function of the subunit to which the profile was prepared. To be significant, the matching has an E-value of 0.01 or less, and further use of “match” is understood to be with this E-value criterion. Thus diol/glycerol dehydratase subunits that may be used in the present invention are proteins that match the Profile HMMs, that were prepared using the proteins with SEQ ID NOs listed above, with an E-value of 0.01 or less.

Proteins that are full length and have functional linkage to the large subunit of diol/glycerol dehydratases, through matching the large subunit Profile HMM, include but are not limited to, proteins with SEQ ID NOs; 93, 167, 170, 173, 176, 179, 182, 185, 188, 191, 194, 197, 200, 203, 206, 209, 212, 215, 218, 221, 224, 227, 130, 243, 254, 255, 256, 257, 258, and 259. Proteins that are full length and have functional linkage to the medium subunit of diol/glycerol dehydratases, through matching the medium subunit Profile HMM, include but are not limited to, proteins with SEQ ID NOs; 95, 168, 171, 174, 177, 180, 183, 186, 189, 192, 195, 198, 201, 204, 207, 210, 213, 216, 219, 222, 225, 228, 231, 244, 250, 252, 260, 261, 262, 263, 364, 265, 266, and 167. Proteins that are full length and have functional linkage to the small subunit of diol/glycerol dehydratases, through matching the small subunit Profile HMM, include but are not limited to, proteins with SEQ ID NOs:97, 169, 172, 175, 178, 181, 184, 187, 190, 193, 196, 199, 202, 205, 208, 211, 214, 217, 220, 223, 226, 229, 232, 234, 236, 238, 240, 242, 245, 248, 249, 251, 253, 268, 270, 271, 272, 273, and 274. In addition, proteins that are fused full length large and medium subunits that have functional linkage to the large and medium subunits of diol/glycerol dehydratases, through matching the large and medium subunit Profiles HMM, include but are not limited to, proteins with SEQ ID NOs; 233, 235, 237, 239, 241, 246, and 247.

Since the Profile HMMs described above provide a structure/function relationship for diol/glycerol dehydratases, newly identified proteins that match these profiles may also be used in the present invention. In addition, diol/glycerol dehydratase subunit protein sequences that may be used in the present invention include proteins with amino acid changes that have minimal effects on subunit function, which are substantially similar to the sequences of the SEQ ID NOs listed above. It is well known in the art that substitution of a chemically equivalent amino acid at a given site which does not effect the functional properties of the encoded protein is common. For the purposes of the present invention substitutions providing substantially similar proteins are defined as exchanges within one of the following five groups:

-   1. Small aliphatic, nonpolar or slightly polar residues: Ala, Ser,     Thr (Pro, Gly); -   2. Polar, negatively charged residues and their amides: Asp, Asn,     Glu, Gln; -   3. Polar, positively charged residues: His, Arg, Lys; -   4. Large aliphatic, nonpolar residues: Met, Leu, Ile, Val (Cys); and -   5. Large aromatic residues: Phe, Tyr, Trp.     Thus, substitutions of one amino acid for another in these groups     can be expected to produce a functionally equivalent protein. In     many cases, changes which result in alteration of the N-terminal and     C-terminal portions of the protein would also not be expected to     alter the activity of the protein.

Substantially similar proteins to those of SEQ IDs that match the Profile HMMs may be 90% or 95% identical in amino acid sequence to one of the matching proteins, and these may be used in the present invention.

One skilled in the art can readily identify a set of three subunits that may be used together to provide a functional diol/glycerol dehydratase. Particularly suitable is a combination of a large, medium and small subunit from the same strain of organism, whose coding regions are located near one another in the genome. These subunits would be most likely to form a natural diol or glycerol dehydratase. Many large, medium, and small, subunits are grouped in this manner in Table 2. A combination of subunits from closely related strains or species is suitable for composing a diol dehydratase or a glycerol dehydratase. Any combination of subunits that catalyzes the conversion of 2,3-butanediol to 2-butanone may be used. Effective subunit combinations may readily be determined by one skilled in the art through amino acid sequence comparisons and/or functional assays.

Accordingly the invention provides diol and glycerol dehydratase enzymes having amino acid sequences comprising full length large, medium and small subunits that each give an E-value parameter of 0.01 or less when queried using a Profile Hidden Markov Model prepared using the large subunits of SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164; the medium subunits of SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; and the small subunits of SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; each query being carried out using the hmmsearch algorithm wherein the Z parameter is set to 1 billion.

Alternatively the invention provides diol and glycerol dehydratase enzymes having amino acid sequences identified by a process comprising a) generating a Profile Hidden Markov Model from the alignment of the amino acid sequences corresponding to the large, medium and small subunits of diol and glycerol dehydratase enzymes wherein;

-   -   i) the large subunit comprises an amino acid sequence selected         from the group consisting of SEQ ID NOs: 8, 99, 105, 135, 138,         141, 146, and 164;     -   ii) the medium subunit comprises an amino acid sequence selected         from the group consisting of SEQ ID NOs: 10, 101, 107, 136, 139,         142, 148, and 165; and     -   iii) the small subunit comprises an amino acid sequence selected         from the group consisting of SEQ ID NOs:12, 103, 109, 137, 140,         143, 150, and 166;     -   b) querying at least one public database of protein sequences         containing sequences of diol and glycerol dehydratases with the         Profile Hidden Markov Model of (a) using the hmmsearch algorithm         wherein the Z parameter is set to 1 billion and the E-value         parameter is set to 0.01, to identify a first data set of diol         and glycerol dehydratase amino acid sequences; and     -   c) removing any partial sequences from the first data set of (b)         to generate a second data set of diol and glycerol dehydratase         amino acid sequences, wherein diol dehydratase and the glycerol         dehydratase enzymes are identified.

With respect to large subunits of the diol and glycerol dehydratases of the invention the enzymes may comprise a large subunit comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 93, 99, 105, 135, 138, 141, 146, 164, 167, 170, 173, 176, 179, 182, 185, 188, 191, 194, 197, 200, 203, 206, 209, 212, 215, 218, 221, 224, 227, 130, 243, 254, 255, 256, 257, 258 and 259, based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.

With respect to the medium subunits of the diol and glycerol dehydratases of the invention the enzymes may comprise a medium subunit comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 10, 95, 101, 107, 136, 139, 142, 148, 165, 168, 171, 174, 177, 180, 183, 186, 189, 192, 195, 198, 201, 204, 207, 210, 213, 216, 219, 222, 225, 228, 231, 244, 250, 252, 260, 261, 262, 263, 364, 265, 266, and 167 based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.

With respect to the small subunits of the diol and glycerol dehydratases of the invention the enzymes may comprise a medium subunit comprising a small subunit comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 12, 97, 103, 109, 137, 140, 143, 150, 166, 169, 172, 175, 178, 181, 184, 187, 190, 193, 196, 199, 202, 205, 208, 211, 214, 217, 220, 223, 226, 229, 232, 234, 236, 238, 240, 242, 245, 248, 249, 251, 253, 268, 270, 271, 272, 273, and 274, based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.

Alternatively the diol dehydratase or glycerol dehydratase may comprise fused large, medium and small subunits comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 233, 235, 237, 239, 241, 246, and 247, based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.

Alternatively the diol dehydratase or glycerol dehydratase enzymes may comprise a fused large, medium and small subunits and has at least 95% identity to an amino acid sequence comprising all three of the amino acid sequences encoding large, medium and small subunits, selected from the group consisting of:

a) SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12;

b) SEQ ID NO:93, SEQ ID NO:95, and SEQ ID NO:97;

c) SEQ ID NO:99, SEQ ID NO:101, and SEQ ID NO:103;

d) SEQ ID NO:105, SEQ ID NO:107, and SEQ ID NO:109;

e) SEQ ID NO:135, SEQ ID NO:136, and SEQ ID NO:137;

f) SEQ ID NO:138, SEQ ID NO:139, and SEQ ID NO:140;

g) SEQ ID NO:146, SEQ ID NO:148, and SEQ ID NO:150;

h) SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:143; and

i) SEQ ID NO:164, SEQ ID NO:165, and SEQ ID NO:166;

based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.

Pathway 4: (a) pyruvate to alpha-acetolactate

This substrate to product conversion is the same as described above for Pathway 1.

(k) alpha-acetolactate to 2,3-dihydroxy-2-methylbutanoic acid

The substrate to product conversion of acetolactate (I) to 2,3-dihydroxy-2-methylbutanoic acid (IX) is not known in the art. However, the product of this conversion has been reported as a component of fermentation broths (Ziadi et al. (1973) Comptes Rendus des Seances de l'Academie des Sciences, Serie D: Sciences Naturelles 276:965-8), but the mechanism of formation is unknown. The likely mechanism of formation is reduction of acetolactate with NADH or NADPH as the electron donor. To utilize this pathway for production of 2-butanol, an enzyme catalyzing this reaction needs to be identified or engineered. However, the precedent for enzymatic reduction of ketones to alcohols is well established.

(l) 2,3-dihydroxy-2-methylbutanoic acid to 2-hydroxy-2-methyl-3-phosphobutanoic acid

There are no enzymes known that catalyze the substrate to product conversion of 2,3-dihydroxy-2-methylbutanoic acid (IX) to 2-hydroxy-2-methyl-3-phosphobutanoic acid (X). However, there are a large number of kinases in Nature that possess varying specificity. It is therefore likely that an enzyme could be isolated or engineered with this activity.

(m) 2-hydroxy-2-methyl-3-phosphobutanoic acid to 2-butanone

There are no known enzymes that catalyze the substrate to product conversion of 2-hydroxy-2-methyl-3-phosphobutanoic acid (X) to 2-butanone (V). The combination of this reaction with the previous one is very similar to the multi-step reaction catalyzed by mevalonate-5-pyrophosphate (M5PP) decarboxylase, which consists of initial phosphorylation of M5PP to 3-phosphomevalonate-5-PP, followed by decarboxylation-dependent elimination of phosphate (Alvear et al. (1982) Biochemistry 21:4646-4650).

(f) 2-butanone to 2-butanol

This substrate to product conversion is the same as described above for Pathway 1.

Thus, in providing multiple recombinant pathways from pyruvate to 2-butanol, there exists a number of choices to fulfill the individual conversion steps, and the person of skill in the art will be able to utilize publicly available sequences and sequences disclosed herein to construct the relevant pathways. A listing of a representative number of genes known in the art and useful in the construction of 2-butanol biosynthetic pathways is given above in Tables 1 and 2.

Microbial Hosts for 2-Butanol and 2-Butanone Production

Microbial hosts for 2-butanol or 2-butanone production may be selected from bacteria, cyanobacteria, filamentous fungi and yeasts. The microbial host used for 2-butanol or 2-butanone production should be tolerant to the product produced, so that the yield is not limited by toxicity of the product to the host. The selection of a microbial host for 2-butanol production is described in detail below. The same criteria apply to the selection of a host for 2-butanone production.

Microbes that are metabolically active at high titer levels of 2-butanol are not well known in the art. Although butanol-tolerant mutants have been isolated from solventogenic Clostridia, little information is available concerning the butanol tolerance of other potentially useful bacterial strains. Most of the studies on the comparison of alcohol tolerance in bacteria suggest that butanol is more toxic than ethanol (de Cavalho et al., Microsc. Res. Tech. 64:215-22 (2004) and Kabelitz et al., FEMS Microbiol. Lett. 220:223-227 (2003)). Tomas et al. (J. Bacteriol. 186:2006-2018 (2004)) report that the yield of 1-butanol during fermentation in Clostridium acetobutylicum may be limited by butanol toxicity. The primary effect of 1-butanol on Clostridium acetobutylicum is disruption of membrane functions (Hermann et al., Appl. Environ. Microbiol. 50:1238-1243 (1985)).

The microbial hosts selected for the production of 2-butanol should be tolerant to 2-butanol and should be able to convert carbohydrates to 2-butanol using the introduced biosynthetic pathway. The criteria for selection of suitable microbial hosts include the following: intrinsic tolerance to 2-butanol, high rate of carbohydrate utilization, availability of genetic tools for gene manipulation, and the ability to generate stable chromosomal alterations.

Suitable host strains with a tolerance for 2-butanol may be identified by screening based on the intrinsic tolerance of the strain. The intrinsic tolerance of microbes to 2-butanol may be measured by determining the concentration of 2-butanol that is responsible for 50% inhibition of the growth rate (IC50) when grown in a minimal medium. The IC50 values may be determined using methods known in the art. For example, the microbes of interest may be grown in the presence of various amounts of 2-butanol and the growth rate monitored by measuring the optical density at 600 nanometers. The doubling time may be calculated from the logarithmic part of the growth curve and used as a measure of the growth rate. The concentration of 2-butanol that produces 50% inhibition of growth may be determined from a graph of the percent inhibition of growth versus the 2-butanol concentration. Preferably, the host strain should have an IC50 for 2-butanol of greater than about 0.5%. More suitable is a host strain with an IC50 for 2-butanol that is greater than about 1.5%. Particularly suitable is a host strain with an IC50 for 2-butanol that is greater than about 2.5%.

The microbial host for 2-butanol production should also utilize glucose and/or other carbohydrates at a high rate. Most microbes are capable of utilizing carbohydrates. However, certain environmental microbes cannot efficiently use carbohydrates, and therefore would not be suitable hosts.

The ability to genetically modify the host is essential for the production of any recombinant microorganism. Modes of gene transfer technology that may be used include by electroporation, conjugation, transduction or natural transformation. A broad range of host conjugative plasmids and drug resistance markers are available. The cloning vectors used with an organism are tailored to the host organism based on the nature of antibiotic resistance markers that can function in that host.

The microbial host also may be manipulated in order to inactivate competing pathways for carbon flow by inactivating various genes. This requires the availability of either transposons or chromosomal integration vectors to direct inactivation. Additionally, production hosts that are amenable to chemical mutagenesis may undergo improvements in intrinsic 2-butanol tolerance through chemical mutagenesis and mutant screening.

Based on the criteria described above, suitable microbial hosts for the production of 2-butanol and 2-butanone include, but are not limited to, members of the genera Clostridium, Zymomonas, Escherichia, Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Pediococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Pichia, Candida, Hansenula and Saccharomyces. Preferred hosts include: Escherichia coli, Alcaligenes eutrophus, Bacillus licheniformis, Paenibacillus macerans, Rhodococcus erythropolis, Pseudomonas putida, Lactobacillus plantarum, Enterococcus faecium, Enterococcus gallinarium, Enterococcus faecalis, Pediococcus pentosaceus, Pediococcus acidilactici, Bacillus subtilis and Saccharomyces cerevisiae.

Construction of Production Host

Recombinant organisms containing the necessary genes that encode the enzymatic pathway for the conversion of a fermentable carbon substrate to 2-butanol or 2-butanone may be constructed using techniques well known in the art. In the present invention, genes encoding the enzymes of the 2-butanol biosynthetic Pathway 3: acetolactate synthase, acetolactate decarboxylase, butanediol dehydrogenase, butanediol dehydratase, and butanol dehydrogenase; or 2-butanone biosynthetic Pathway 3 omitting the butanol dehydrogenase, may be isolated from various sources, as described above.

Methods of obtaining desired genes from a bacterial genome are common and well known in the art of molecular biology. For example, if the sequence of the gene is known, primers may be designed and the desired sequence amplified using standard primer-directed amplification methods such as polymerase chain reaction (U.S. Pat. No. 4,683,202) to obtain amounts of DNA suitable for cloning into expression vectors. If a gene that is heterologous to a known sequence is to be isolated, suitable genomic libraries may be created by restriction endonuclease digestion and may be screened with probes having complementary sequence to the desired gene sequence. Once the sequence is isolated, the DNA may be amplified using standard primer-directed amplification methods such as polymerase chain reaction (U.S. Pat. No. 4,683,202) to obtain amounts of DNA suitable for cloning into expression vectors, which are then transformed into appropriate host cells.

In addition, given the amino acid sequence of a protein with desired enzymatic activity, the coding sequence may be ascertained by reverse translating the protein sequence. A DNA fragment containing the coding sequence may be prepared synthetically and cloned into an expression vector, then transformed into the desired host cell.

In preparing a synthetic DNA fragment containing a coding sequence, this sequence may be optimized for expression in the target host cell. Tools for codon optimization for expression in a heterologous host are readily available. Some tools for codon optimization are available based on the GC content of the host organism. The GC contents of some exemplary microbial hosts are given Table 3.

TABLE 3 GC Contents of Microbial Hosts Strain % GC B. licheniformis 46 B. subtilis 42 C. acetobutylicum 37 E. coli 50 P. putida 61 A. eutrophus 61 Paenibacillus macerans 51 Rhodococcus erythropolis 62 Brevibacillus 50 Paenibacillus polymyxa 50

Once the relevant pathway genes are identified and isolated they may be transformed into suitable expression hosts by means well known in the art. Vectors useful for the transformation of a variety of host cells are common and commercially available from companies such as EPICENTRE® (Madison, Wis.), Invitrogen Corp. (Carlsbad, Calif.), Stratagene (La Jolla, Calif.), and New England Biolabs, Inc. (Beverly, Mass.). Typically the vector contains a selectable marker and sequences allowing autonomous replication or chromosomal integration in the desired host. In addition, suitable vectors comprise a promoter region which harbors transcriptional initiation controls and a transcriptional termination control region, between which a coding region DNA fragment may be inserted, to provide expression of the inserted coding region. Both control regions may be derived from genes homologous to the transformed host cell, although it is to be understood that such control regions may also be derived from genes that are not native to the specific species chosen as a production host.

Initiation control regions or promoters, which are useful to drive expression of the relevant pathway coding regions in the desired host cell are numerous and familiar to those skilled in the art. Virtually any promoter capable of driving these genetic elements is suitable for the present invention including, but not limited to, promoters derived from the following genes: CYC1, HIS3, GAL1, GAL10, ADH1, PGK, PHO5, GAPDH, ADC1, TRP1, URA3, LEU2, ENO, TPI, CUP1, FBA, GPD, and GPM (useful for expression in Saccharomyces); AOX1 (useful for expression in Pichia); as well as the lac, ara, tet, trp, IP_(L), IP_(R), T7, tac, and trc promoters (useful for expression in Escherichia coli, Alcaligenes, and Pseudomonas); the amy, apr, and npr promoters, and various phage promoters useful for expression in Bacillus subtilis, Bacillus licheniformis, and Paenibacillus macerans; nisA (useful for expression Gram-positive bacteria, Eichenbaum et al. Appl. Environ. Microbiol. 64(8):2763-2769 (1998)); and the synthetic P11 promoter (useful for expression in Lactobacillus plantarum, Rud et al., Microbiology 152:1011-1019 (2006)).

Termination control regions may also be derived from various genes native to the preferred hosts. Optionally, a termination site may be unnecessary, however, it is most preferred if included.

Certain vectors are capable of replicating in a broad range of host bacteria and can be transferred by conjugation. The complete and annotated sequence of pRK404 and three related vectors: pRK437, pRK442, and pRK442(H), are available. These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria (Scott et al., Plasmid 50(1):74-79 (2003)). Several plasmid derivatives of broad-host-range Inc P4 plasmid RSF1010 are also available with promoters that can function in a range of Gram-negative bacteria. Plasmid pAYC36 and pAYC37, have active promoters along with multiple cloning sites to allow for heterologous gene expression in Gram-negative bacteria.

Chromosomal gene replacement tools are also widely available. For example, a thermosensitive variant of the broad-host-range replicon pWV101 has been modified to construct a plasmid pVE6002 which can be used to effect gene replacement in a range of Gram-positive bacteria (Maguin et al., J. Bacteriol. 174(17):5633-5638 (1992)). Additionally, in vitro transposomes are available from commercial sources such as EPICENTRE® to create random mutations in a variety of genomes.

The expression of a 2-butanol biosynthetic pathway in various preferred microbial hosts is described in more detail below. For the expression of a 2-butanone biosynthetic pathway, the same description applies, but the final substrate to product conversion of 2-butanone to 2-butanol is omitted.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in E. coli

Vectors useful for the transformation of E. coli are common and commercially available from the companies listed above. For example, the genes of a 2-butanol biosynthetic pathway may be isolated from various sources, as described above, cloned onto a modified pUC19 vector and transformed into E. coli NM522, as described in Examples 6 and 7. Alternatively, the genes encoding a 2-butanol biosynthetic pathway may be divided into multiple operons, cloned onto expression vectors, and transformed into various E. coli strains, as described in Examples 9, 10, and 11. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Rhodococcus erythropolis

A series of E. coli-Rhodococcus shuttle vectors are available for expression in R. erythropolis, including, but not limited to pRhBR17 and pDA71 (Kostichka et al., Appl. Microbiol. Biotechnol. 62:61-68 (2003)). Additionally, a series of promoters are available for heterologous gene expression in R. erythropolis (see for example Nakashima et al., Appl. Environ. Microbiol. 70:5557-5568 (2004), and Tao et al., Appl. Microbiol. Biotechnol. 2005, DOI 10.1007/s00253-005-0064). Targeted gene disruptions in chromosomal genes of R. erythropolis may be created using the methods described by Tao et al., supra, and Brans et al. (Appl. Envion. Microbiol. 66: 2029-2036 (2000)).

The heterologous genes required for the production of 2-butanol, as described above, may be cloned initially in pDA71 or pRhBR71 and transformed into E. coli. The vectors may then be transformed into R. erythropolis by electroporation, as described by Kostichka et al., supra. The recombinants may be grown in synthetic medium containing glucose and the production of 2-butanol can be followed using fermentation methods known in the art. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in B. Subtilis

Methods for gene expression and creation of mutations in B. subtilis are also well known in the art. For example, the genes of a 2-butanol biosynthetic pathway may be isolated from various sources, as described above, cloned into a modified E. coli-Bacillus shuttle vector and transformed into Bacillus subtilis BE1010, as described in Example 8, The desired genes may be cloned into a Bacillus expression vector and transformed into a strain to make a production host. Alternatively, the genes may be integrated into the Bacillus chromosome using conditional replicons or suicide vectors that are known to one skilled in the art. For example, the Bacillus Genetic Stock Center carries numerous integration vectors. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in B. licheniformis

Most of the plasmids and shuttle vectors that replicate in B. subtilis may be used to transform B. licheniformis by either protoplast transformation or electroporation. The genes required for the production of 2-butanol may be cloned in plasmids pBE20 or pBE60 derivatives (Nagarajan et al., Gene 114:121-126 (1992)). Methods to transform B. licheniformis are known in the art (for example see Fleming et al. Appl. Environ. Microbiol., 61(11):3775-3780 (1995)). The plasmids constructed for expression in B. subtilis may be transformed into B. licheniformis to produce a recombinant microbial host that produces 2-butanol. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Paenibacillus macerans

Plasmids may be constructed as described above for expression in B. subtilis and used to transform Paenibacillus macerans by protoplast transformation to produce a recombinant microbial host that produces 2-butanol. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Alcaligenes (Ralstonia) eutrophus

Methods for gene expression and creation of mutations in Alcaligenes eutrophus are known in the art (see for example Taghavi et al., Appl. Environ. Microbiol., 60(10):3585-3591 (1994)). The genes for a 2-butanol biosynthetic pathway may be cloned in any of the broad host range vectors described above, and electroporated into Alcaligenes eutrophus to generate recombinants that produce 2-butanol. The poly(hydroxybutyrate) pathway in Alcaligenes has been described in detail, a variety of genetic techniques to modify the Alcaligenes eutrophus genome are known, and those tools can be applied for engineering a 2-butanol biosynthetic pathway. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Pseudomonas putida

Methods for gene expression in Pseudomonas putida are known in the art (see for example Ben-Bassat et al., U.S. Pat. No. 6,586,229, which is incorporated herein by reference). The genes of a 2-butanol biosynthetic pathway may be inserted into pPCU18, and this ligated DNA may be electroporated into electrocompetent Pseudomonas putida DOT-T1 C5aAR1 cells to generate recombinants that produce 2-butanol. The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Lactobacillus plantarum

The Lactobacillus genus belongs to the Lactobacillales family and many plasmids and vectors used in the transformation of Bacillus subtilis and Streptococcus may be used for Lactobacillus. Non-limiting examples of suitable vectors include pAMβ1 and derivatives thereof (Renault et al., Gene 183:175-182 (1996); and O'Sullivan et al., Gene 137:227-231 (1993)); pMBB1 and pHW800, a derivative of pMBB1 (Wyckoff et al. Appl. Environ. Microbiol. 62:1481-1486 (1996)); pMG1, a conjugative plasmid (Tanimoto et al., J. Bacteriol. 184:5800-5804 (2002)); pNZ9520 (Kleerebezem et al., Appl. Environ. Microbiol. 63:4581-4584 (1997)); pAM401 (Fujimoto et al., Appl. Environ. Microbiol. 67:1262-1267 (2001)); and pAT392 (Arthur et al., Antimicrob. Agents Chemother. 38:1899-1903 (1994)). Several plasmids from Lactobacillus plantarum have also been reported (van Kranenburg et al., Appl. Environ. Microbiol. 71(3):1223-1230 (2005)).

The various genes for a 2-butanol biosynthetic pathway may be assembled into any suitable vector, such as those described above. The codons can be optimized for expression based on the codon index deduced from the genome sequences of Lactobacillus plantarum or Lactobacillus arizonensis. The plasmids may be introduced into the host cell using methods known in the art, such as electroporation (Cruz-Rodz et al. Molecular Genetics and Genomics 224:1252-154 (1990), Bringel, et al. Appl. Microbiol. Biotechnol. 33: 664-670 (1990), Alegre et al., FEMS Microbiology letters 241:73-77 (2004)), and conjugation (Shrago et al., Appl. Environ. Microbiol. 52:574-576 (1986)). The 2-butanol biosynthetic pathway genes can also be integrated into the chromosome of Lactobacillus using integration vectors (Hols et al., Appl. Environ. Microbiol. 60:1401-1403 (1990), Jang et al., Micro. Lett. 24:191-195 (2003)). The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Enterococcus faecium, Enterococcus gallinarium, and Enterococcus faecalis

The Enterococcus genus belongs to the Lactobacillales family and many plasmids and vectors used in the transformation of Lactobacillus, Bacillus subtilis, and Streptococcus, described above, may be used for Enterococcus. Expression vectors for E. faecalis using the nisA gene from Lactococcus may also be used (Eichenbaum et al., Appl. Environ. Microbiol. 64:2763-2769 (1998). Additionally, vectors for gene replacement in the E. faecium chromosome may be used (Nallaapareddy et al., Appl. Environ. Microbiol. 72:334-345 (2006)).

The various genes for a 2-butanol biosynthetic pathway may be assembled into any suitable vector, such as those described above. The codons can be optimized for expression based on the codon index deduced from the genome sequences of Enterococcus faecalis or Enterococcus faecium. The plasmids may be introduced into the host cell using methods known in the art, such as electroporation, as described by Cruz-Rodz et al. (Molecular Genetics and Genomics 224:1252-154 (1990)) or conjugation, as described by Tanimoto et al. (J. Bacteriol. 184:5800-5804 (2002)) and Grohamann et al. (Microbiol. Mol. Biol. Rev. 67:277-301 (2003)). The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Expression of a 2-Butanol or 2-Butanone Biosynthetic Pathway in Pediococcus pentosaceus and Pediococcus acidilactici,

The Pediococcus genus belongs to the Lactobacillales family and many plasmids and vectors used in the transformation of Bacillus subtilis and Streptococcus, described above, may be used for Pediococcus. A non-limiting example of a suitable vector is pHPS9 (Bukhtiyarova et al. Appl. Environ. Microbiol. 60:3405-3408 (1994)). Several plasmids from Pediococcus have also been reported (Alegre et al., FEMS Microbiol. Lett. 250:151-156 (2005); Shareck et al. Crit. Rev Biotechnol. 24:155-208 (2004)).

The genes for a 2-butanol biosynthetic pathway may be assembled into any suitable vector, such as those described above. The codons can be optimized for expression based on the codon index deduced from the genome sequence of Pediococcus pentosaceus. The plasmids may be introduced into the host cell using methods known in the art, such as electroporation (see for example, Osmanagaoglu et al., J. Basic Microbiol. 40:233-241 (2000); Alegre et al., FEMS Microbiol. Lett. 250:151-156 (2005)) and conjugation (Gonzalez and Kunka, Appl. Environ. Microbiol. 46:81-89 (1983)). The 2-butanol biosynthetic pathway genes can also be integrated into the chromosome of Pediococcus using integration vectors (Davidson et al. Antonie van Leeuwenhoek 70:161-183 (1996)). The 2-butanone biosynthesis pathway may be similarly expressed, omitting the butanol dehydrogenase.

Fermentation Media

Fermentation media in the present invention must contain suitable carbon substrates. Suitable substrates may include but are not limited to monosaccharides such as glucose and fructose, oligosaccharides such as lactose or sucrose, polysaccharides such as starch or cellulose or mixtures thereof and unpurified mixtures from renewable feedstocks such as cheese whey permeate, cornsteep liquor, sugar beet molasses, and barley malt. Additionally the carbon substrate may also be one-carbon substrates such as carbon dioxide, or methanol for which metabolic conversion into key biochemical intermediates has been demonstrated. In addition to one and two carbon substrates, methylotrophic organisms are also known to utilize a number of other carbon containing compounds such as methylamine, glucosamine and a variety of amino acids for metabolic activity. For example, methylotrophic yeasts are known to utilize the carbon from methylamine to form trehalose or glycerol (Bellion et al., Microb. Growth C1-Compd., [Int. Symp.], 7th (1993), 415-32, Editor(s): Murrell, J. Collin; Kelly, Don P. Publisher: Intercept, Andover, UK). Similarly, various species of Candida will metabolize alanine or oleic acid (Sulter et al., Arch. Microbiol. 153:485-489 (1990)). Hence it is contemplated that the source of carbon utilized in the present invention may encompass a wide variety of carbon containing substrates and will only be limited by the choice of organism.

Although it is contemplated that all of the above mentioned carbon substrates and mixtures thereof are suitable in the present invention, preferred carbon substrates are glucose, fructose, and sucrose, as well as mixtures of any of these sugars. Sucrose may be obtained from feedstocks such as sugar cane, sugar beets, cassava, and sweet sorghum. Glucose and dextrose may be obtained through saccharification of starch based feedstocks including grains such as corn, wheat, rye, barley, and oats.

In addition, fermentable sugars may be obtained from cellulosic and lignocellulosic biomass through processes of pretreatment and saccharification, as described, for example, in co-owned and co-pending US patent application US20070031918A1, which is herein incorporated by reference. Biomass refers to any cellulosic or lignocellulosic material and includes materials comprising cellulose, and optionally further comprising hemicellulose, lignin, starch, oligosaccharides and/or monosaccharides. Biomass may also comprise additional components, such as protein and/or lipid. Biomass may be derived from a single source, or biomass can comprise a mixture derived from more than one source; for example, biomass could comprise a mixture of corn cobs and corn stover, or a mixture of grass and leaves. Biomass includes, but is not limited to, bioenergy crops, agricultural residues, municipal solid waste, industrial solid waste, sludge from paper manufacture, yard waste, wood and forestry waste. Examples of biomass include, but are not limited to, corn grain, corn cobs, crop residues such as corn husks, corn stover, grasses, wheat, wheat straw, barley, barley straw, hay, rice straw, switchgrass, waste paper, sugar cane bagasse, sorghum, soy, components obtained from milling of grains, trees, branches, roots, leaves, wood chips, sawdust, shrubs and bushes, vegetables, fruits, flowers and animal manure.

In addition to an appropriate carbon source, fermentation media must contain suitable minerals, salts, cofactors, buffers and other components, known to those skilled in the art, suitable for the growth of the cultures and promotion of an enzymatic pathway necessary for 2-butanol or 2-butanone production.

Culture Conditions

Typically cells are grown at a temperature in the range of about 25° C. to about 40° C. in an appropriate medium. Suitable growth media in the present invention are common commercially prepared media such as Luria Bertani (LB) broth, Sabouraud Dextrose (SD) broth or Yeast Medium (YM) broth. Other defined or synthetic growth media may also be used, and the appropriate medium for growth of the particular microorganism will be known by one skilled in the art of microbiology or fermentation science. The use of agents known to modulate catabolite repression directly or indirectly, e.g., cyclic adenosine 2′:3′-monophosphate, may also be incorporated into the fermentation medium.

Suitable pH ranges for the fermentation are between pH 5.0 to pH 9.0, where pH 6.0 to pH 8.0 is preferred as the initial condition.

Fermentations may be performed under aerobic or anaerobic conditions, where anaerobic or microaerobic conditions are preferred.

Industrial Batch and Continuous Fermentations

The present process employs a batch method of fermentation. A classical batch fermentation is a closed system where the composition of the medium is set at the beginning of the fermentation and not subject to artificial alterations during the fermentation. Thus, at the beginning of the fermentation the medium is inoculated with the desired organism or organisms, and fermentation is permitted to occur without adding anything to the system. Typically, however, a “batch” fermentation is batch with respect to the addition of carbon source and attempts are often made at controlling factors such as pH and oxygen concentration. In batch systems the metabolite and biomass compositions of the system change constantly up to the time the fermentation is stopped. Within batch cultures cells moderate through a static lag phase to a high growth log phase and finally to a stationary phase where growth rate is diminished or halted. If untreated, cells in the stationary phase will eventually die. Cells in log phase generally are responsible for the bulk of production of end product or intermediate.

A variation on the standard batch system is the fed-batch system. Fed-batch fermentation processes are also suitable in the present invention and comprise a typical batch system with the exception that the substrate is added in increments as the fermentation progresses. Fed-batch systems are useful when catabolite repression is apt to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the media. Measurement of the actual substrate concentration in fed-batch systems is difficult and is therefore estimated on the basis of the changes of measurable factors such as pH, dissolved oxygen and the partial pressure of waste gases such as CO₂. Batch and fed-batch fermentations are common and well known in the art and examples may be found in Thomas D. Brock in Biotechnology: A Textbook of Industrial Microbiology, Second Edition (1989) Sinauer Associates, Inc., Sunderland, Mass., or Deshpande, Mukund V., Appl. Biochem. Biotechnol., 36:227, (1992), herein incorporated by reference.

Although the present invention is performed in batch mode it is contemplated that the method would be adaptable to continuous fermentation methods. Continuous fermentation is an open system where a defined fermentation medium is added continuously to a bioreactor and an equal amount of conditioned media is removed simultaneously for processing. Continuous fermentation generally maintains the cultures at a constant high density.

Continuous fermentation allows for the modulation of one factor or any number of factors that affect cell growth or end product concentration. For example, one method will maintain a limiting nutrient such as the carbon source or nitrogen level at a fixed rate and allow all other parameters to moderate. In other systems a number of factors affecting growth can be altered continuously while the cell concentration, measured by the turbidity of the culture medium, is kept constant. Continuous systems strive to maintain steady state growth conditions and thus the cell loss due to the medium being drawn off must be balanced against the cell growth rate in the fermentation. Methods of modulating nutrients and growth factors for continuous fermentation processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology and a variety of methods are detailed by Brock, supra.

It is contemplated that the present invention may be practiced using either batch, fed-batch or continuous processes and that any known mode of fermentation would be suitable. Additionally, it is contemplated that cells may be immobilized on a substrate as whole cell catalysts and subjected to fermentation conditions for 2-butanol or 2-butanone production.

Methods for 2-Butanol and 2-Butanone Isolation from the Fermentation Medium

The bioproduced 2-butanol may be isolated from the fermentation medium using methods known in the art for ABE fermentations (see for example, Durre, Appl. Microbiol. Biotechnol. 49:639-648 (1998), Groot et al., Process Biochem. 27:61-75 (1992), and references therein). For example, solids may be removed from the fermentation medium by centrifugation, filtration, decantation, or the like. Then, the 2-butanol may be isolated from the fermentation medium using methods such as distillation, azeotropic distillation, liquid-liquid extraction, adsorption, gas stripping, membrane evaporation, or pervaporation. These same methods may be adapted to isolate bioproduced 2-butanone from the fermentation medium.

EXAMPLES

The present invention is further defined in the following Examples. It should be understood that these Examples, while indicating a preferred embodiment of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and, without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various uses and conditions.

General Methods

Standard recombinant DNA and molecular cloning techniques described in the Examples are well known in the art and are described by Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y., (1989) (Maniatis) and by T. J. Silhavy, M. L. Bennan, and L. W. Enquist, Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1984) and by Ausubel, F. M. et al., Current Protocols in Molecular Biology, pub. by Greene Publishing Assoc. and Wiley-Interscience (1987).

Materials and methods suitable for the maintenance and growth of bacterial cultures are well known in the art. Techniques suitable for use in the following Examples may be found as set out in Manual of Methods for General Bacteriology (Phillipp Gerhardt, R. G. E. Murray, Ralph N. Costilow, Eugene W. Nester, Willis A. Wood, Noel R. Krieg and G. Briggs Phillips, eds), American Society for Microbiology, Washington, D.C. (1994)) or by Thomas D. Brock in Biotechnology: A Textbook of Industrial Microbiology, Second Edition, Sinauer Associates, Inc., Sunderland, Mass. (1989). All reagents, restriction enzymes and materials described for the growth and maintenance of bacterial cells were obtained from Aldrich Chemicals (Milwaukee, Wis.), BD Diagnostic Systems (Sparks, Md.), Life Technologies (Rockville, Md.), or Sigma Chemical Company (St. Louis, Mo.) unless otherwise specified. Bacterial strains are obtained from the American Type Culture Collection (ATCC, Manassas, Va.) unless otherwise noted.

-   -   Oligonucleotide primers described in the following Examples are         given in Table 4. All oligonucleotide primers were synthesized         by Sigma-Genosys (Woodlands, Tex.).

TABLE 4 Cloning and Screening Primers Primer SEQ ID Gene Name Sequence NO: Description budB B1 CACCATGGACAAACAGTA 15 budB TCCGGTACGCC forward budB B2 CGAAGGGCGATAGCTTTA 16 budB CCAATCC reverse budA B3 CACCATGAATCATTCTGC 17 budA forward TGAATGCACCTGCG budA B4 GATACTGTTTGTCCATGT 18 budA reverse GACC budC B5 CACCATGAAAAAAGTCGC 19 budC ACTTGTTACC forward budC B6 TTAGTTAAATACCAT 20 budC reverse pddA B7 CACCATGAGATCGA 21 pddABC AAAGATTTG forward pddC B8 CTTAGAGAAGTTAATCGT 22 pddABC CGCC reverse sadh B9 CACCATGAAAGCCCTCCA 23 sadh GTACACC forward sadh B10 CGTCGTGTCATGCCCGG 24 sadh G reverse budA B11 GATCGAATTCGTTTAAACT 25 budABC TAGTTTTCTACCGCACG forward budC B12 GATCGCATGCAAGCTTTC 26 budABC ATATAGTCGGAATTCC reverse pddA B13 GATCGAATTCGTTTAAACA 27 pddABC AAGGAGGTCTGATTCATG forward AGATCG pddC B14 GATCGGATTCTTAATCGT 28 pddABC CGCC reverse sadh B15 GATCGGATCCAAAGGAGG 29 sadh TCGGGCGCATGAAAGCC forward C sadh B16 GATCTCTAGAAAGCTTTC 30 sadh AGCCCGGGACGACC reverse — BenF ACTTTCTTTCGCCTGTTTC 31 — AC — BenBPR CATGAAGCTTGTTTAAACT 32 — CGGTGACCTTGAAAATAA TGAAAACTTATATTGTTTT GAAAATAATGAAAACTTAT ATTG budA BABC F GAGCTCGAATTCAAAGGA 33 budAB GGAAGTGTATATGAATCA forward TTC budAB BAB R GGATCCTCTAGAATTAGT 34 budAB TAAATACCATCCCGCCG reverse budC BC Spe F ACTAGTAAAGGAGGAAAG 40 budC forward AGTATGAAGAAGGTCGCA CT budC BC Xba R TCTAGAAAGCAGGGGCAA 41 budC reverse GCCATGTC pddAB DDo For AAGCTTAAAGGAGGCTGA 44 pddABC-ddrAB C- TTCATGAGATCGAAAAGA forward ddrAB TT pddAB DDo Rev TCTAGATTATTCATCCTGC 45 pddABC-ddrAB C- TGTTCTCC reverse ddrAB chnA ChnA F CATCAATTGACTACGTAG 54 chnA forward TCGTACGTGTAAGGAGGT TTGAAATGGAAAAAATTAT G chnA ChnA R CATGCTAGCCCCGGGTAT 55 chnA reverse CTTCTACTCATTTTTTATTT CG — Top ter F1 CTAGAAGTCAAAAGCCTC 58 forward CGACCGGAGGCTTTTGA — Top ter F2 CTGCTCGAGTTGCTAGC 59 forward AAGTTTAAACAAAAAAAA GCCCGCTCATTAGGCGG GCTGAGCT — Bot ter R1 CAGCCCGCCTAATGAGC 60 reverse GGGCTTTTTTTTGTTTAA AC — Bot ter R2 TTGCTAGCAACTCGAGCA 61 reverse GTCAAAAGCCTCCGGTC GGAGGCTTTTGACTT KA-AT OT872 CTCCGGAATTCATGTCTG 127 Aminoalcohol ACGGACGACTCACCGCA kinase/lyase operon forward KA-AT OT873 TTCCAATGCATTGGCTGC 128 Aminoalcohol AGTTATCTCTGTGCACGA kinase/lyase GTGCCGATGA operon reverse KA OT879 AACAGCCAAGCTTGGCT 129 Aminoalcohol GCAGTCATCGCGCATTCT kinase CCGGG reverse AT OT880 TCTCCGGAATTCATGACG 130 Aminoalcohol TCTGAAATGACAGCGACA lyase GAAG forward pBAD. OT909 GCTAACAGGAGGAAGAA 131 Adds EcoRI HisB TTCATGGGGGGTTCTC site to replace NcoI site pBAD. OT910 GAGAACCCCCCATGAATT 132 Adds EcoRI HisB CTTCCTCCTGTTAGC site to replace NcoI site BudAB N84seqR3 GGACCTGCTTCGCTTTAT 159 reverse CG APT APTfor GCGCGCCCGGGAAGAAG 162 APT forward GAGCTCTTCACCATGAAC AAACCACAGTCTTGG APT APTrev GCGCGCCCGGGTTCATG 163 APT reverse CCACCTCTGCG

TABLE 5 Sequencing Primers Gene- SEQ ID Name Sequence specific NO: M13 Forward GTAAAACGACGGCCAGT — 35 M13 Reverse AACAGCTATGACCATG — 36 N83 SeqF2 GCTGGATTACCAGCTCGACC — 37 N83 SeqF3 CGGACGCATTACCGGCAAAG — 38 N84 Seq R2 GCATCGAGATTATCGGGATG — 65 N84 SeqR4 CGAAGCGAGAGAAGTTATCC — 39 Trc F TTGACAATTAATCATCCGGC all 42 Trc R CTTCTCTCATCCGCCAAAAC all 43 DDko seq F2 GCATGGCGCGGATTTGACGA pddABC- 46 AC ddrAB DDko seq F5 CATTAAAGAGACCAAGTACG pddABC- 47 TG ddrAB DDko seq F7 ATATCCTGGTGGTGTCGTCG pddABC- 48 GCGT ddrAB DDko seq F9 TCTTTGTCACCAACGCCCTG pddABC- 49 CG ddrAB DDko seq R1 GCCCACCGCGCTCGCCGCCG pddABC- 50 CG ddrAB DDko seq R3 CCCCCAGGATGGCGGCTTCG pddABC- 51 GC ddrAB DDko seq R7 GGGCCGACGGCGATAATCAC pddABC- 52 TT ddrAB DDko seq R10 TTCTTCGATCCACTCCTTAA pddABC- 53 CG ddrAB chnSeq F1 CTCAACAGGGTGTAAGTGTA chnA 56 GT chnSeq R1 CGTTTTGATATAGCCAGGAT chnA 57 GT pCL1925 vec F CGGTATCATCAACAGGCTTA all 62 CC pCL1925 vec R1 AGGGTTTTCCCAGTCACGAC all 63 GT pCL1925 vec R2 CGCAATAGTTGGCGAAGTAA all 64 TC APTseqRev GCTAGAGATGATAGC APT 160 APTseqFor GGAAGAGACTATCCAGCG APT 161

Methods for Determining 2-Butanol and 2-Butanone Concentration in Culture Media

The concentration of 2-butanol and 2-butanone in the culture media can be determined by a number of methods known in the art. For example, a specific high performance liquid chromatography (HPLC) method utilized a Shodex SH-1011 column with a Shodex SH-G guard column, both purchased from Waters Corporation (Milford, Mass.), with refractive index (RI) detection. Chromatographic separation was achieved using 0.01 M H₂SO₄ as the mobile phase with a flow rate of 0.5 mL/min and a column temperature of 50° C. Under the conditions used, 2-butanone and 2-butanol had retention times of 39.5 and 44.3 min, respectively. Alternatively, gas chromatography (GC) methods are available. For example, a specific GC method utilized an HP-INNOWax column (30 m×0.53 mm id, 1 μm film thickness, Agilent Technologies, Wilmington, Del.), with a flame ionization detector (FID). The carrier gas was helium at a flow rate of 4.5 mL/min, measured at 150° C. with constant head pressure; injector split was 1:25 at 200° C.; oven temperature was 45° C. for 1 min, 45 to 220° C. at 10° C./min, and 220° C. for 5 min; and FID detection was employed at 240° C. with 26 mL/min helium makeup gas. The retention times of 2-butanone and 2-butanol were 3.61 and 5.03 min, respectively.

2-Butanone can also be detected by derivatization with 3-methyl-2-benzothiazolinone hydrazone (MBTH). An aqueous solution containing 2-butanone is mixed with an equal volume of an aqueous solution of 6 mg/mL MBTH in 375 mM glycine-HCl (pH 2.7) and incubated at 100° C. for 3 min. The resulting MBTH-derivatized samples are analyzed on a 25 cm×4.6 mm (id) Supelosil LC-18-D5 5 μm column (Supelco) using a mobile phase of 55% acetonitrile in water at a flow rate of 1 mL/min. The 2-butanone derivative appears as two peaks (cis and trans isomers) with retention times of approximately 12.3 and 13.3 min and absorbance maxima of 230 and 307 nm.

The meaning of abbreviations is as follows: “s” means second(s), “min” means minute(s), “h” means hour(s), “psi” means pounds per square inch, “nm” means nanometers, “d” means day(s), “μL” means microliter(s), “mL” means milliliter(s), “L” means liter(s), “mm” means millimeter(s), “nm” means nanometers, “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “μmol” means micromole(s)”, “g” means gram(s), “μg” means microgram(s) and “ng” means nanogram(s), “PCR” means polymerase chain reaction, “OD” means optical density, “OD₆₀₀” means the optical density measured at a wavelength of 600 nm, “kDa” means kilodaltons, “g” means the gravitation constant, “bp” means base pair(s), “kbp” means kilobase pair(s), “% w/v” means weight/volume percent, % v/v” means volume/volume percent, “wt %” means percent by weight, “HPLC” means high performance liquid chromatography, and “GC” means gas chromatography. The term “molar selectivity” is the number of moles of product produced per mole of sugar substrate consumed and is reported as a percent.

Example 1 Cloning and Expression of Acetolactate Synthase

The purpose of this Example was to clone and express in E. coli the budB gene that encodes the enzyme acetolactate synthase. The budB gene was amplified from Klebsiella pneumoniae strain ATCC 25955 genomic DNA using PCR.

The budB sequence which encodes acetolactate synthase was amplified from Klebsiella pneumoniae (ATCC 25955) genomic DNA by PCR using the primer pair B1 (SEQ ID NO:15) and B2 (SEQ ID NO:16). Other PCR amplification reagents (e.g. Kod HiFi DNA Polymerase (Novagen Inc., Madison, Wis.; catalog no. 71805-3)) were supplied in manufacturers' kits and used according to the manufacturer's protocol. Klebsiella pneumoniae genomic DNA was prepared using the Gentra Puregene Puregene kit (Gentra Systems, Inc., Minneapolis, Minn.; catalog number D-5000A). Amplification was carried out in a DNA Thermocycler GeneAmp 9700 (PE Applied Biosystems, Foster city, CA). The nucleotide sequence of the open reading frame (ORF) and the predicted amino acid sequence of the enzyme are given as SEQ ID NO:3 and SEQ ID NO:4, respectively.

For expression studies the Gateway cloning technology (Invitrogen Corp., Carlsbad, Calif.) was used. The entry vector pENTR/SD/D-TOPO allows directional cloning and provided a Shine-Dalgarno sequence for the gene of interest. The destination vector pDEST14 used a T7 promoter for expression of the gene with no tag. The forward primer incorporated four bases (CACC) immediately adjacent to the translational start codon to allow directional cloning of the budB acetolactate synthase coding region PCR product into pENTR/SD/D-TOPO (Invitrogen), generating the plasmid pENTRSDD-TOPObudB. The PENTR construct was transformed into E. coli Top10 (Invitrogen) cells and plated according to the manufacturer's recommendations. Transformants were grown overnight and plasmid DNA was prepared using the QIAprep Spin Miniprep kit (Qiagen, Valencia, Calif.; catalog no. 27106) according to the manufacturer's recommendations. To create an expression clone, the budB coding region from PENTRSDD-TOPObudB was transferred to the pDEST 14 vector by in vitro recombination using the LR Clonase mix (Invitrogen, Corp., Carlsbad, Calif.). The resulting vector, pDEST14budB, was transformed into BL-21-AI cells (Invitrogen Corp.). BL-21-AI cells carry a chromosomal copy of the T7 RNA polymerase under control of the arabinose-inducible araBAD promoter.

Transformants are inoculated into LB medium supplemented with 50 μg/mL of ampicillin and grown overnight. An aliquot of the overnight culture is used to inoculate 50 mL of LB medium supplemented with 50 μg/mL of ampicillin. The culture is incubated at 37° C. with shaking until the OD₆₀₀ reaches 0.6-0.8. The culture is split into two 25-mL portions and arabinose is added to one of the flasks to a final concentration of 0.2% w/v. The negative control flask is not induced with arabinose. The flasks are incubated for 4 h at 37° C. with shaking. Cells are harvested by centrifugation and the cell pellets are resuspended in 50 mM MOPS, pH 7.0 buffer. The cells are disrupted either by sonication or by passage through a French Pressure Cell. Each cell lysate is centrifuged yielding the supernatant and the pellet or the insoluble fraction. An aliquot of each fraction (whole cell lysate, from induced and control cells, is resuspended in SDS (MES) loading buffer (Invitrogen), heated to 85° C. for 10 min and subjected to SDS-PAGE analysis (NuPAGE 4-12% Bis-Tris Gel, catalog no. NP0322Box, Invitrogen). A protein of the expected molecular weight, as deduced from the nucleic acid sequence, is present in the induced culture but not in the uninduced control.

Acetolactate synthase activity in the cell free extracts is measured using the method described by Bauerle et al. (Bauerle et al. (1964) Biochim. Biophys. Acta 92:142-149). Protein concentration is measured by either the Bradford method or by the Bicinchoninic Kit (Sigma, catalog no. BCA-1; St. Louis, Mo.) using Bovine serum albumin (BSA) (Bio-Rad, Hercules, Calif.) as the standard.

Example 2 Cloning and Expression of Acetolactate Decarboxylase

The purpose of this Example was to clone and express in E. coli the budA gene that encodes the enzyme acetolactate decarboxylase. The budA gene was amplified from Klebsiella pneumoniae strain ATCC 25955 genomic DNA using PCR.

The budA sequence which encodes acetolactate decarboxylase, was cloned in the same manner as described for budB in Example 1, except that the primers used for PCR amplification were B3 (SEQ ID NO:17) and B4 (SEQ ID NO:18). The nucleotide sequence of the open reading frame (ORF) and the predicted amino acid sequence of the enzyme are given as SEQ ID NO:1 and SEQ ID NO:2, respectively. The resulting plasmid was named pENTRSDD-TOPObudA.

Acetolactate decarboxylase activity in the cell free extracts is measured using the method described by Bauerle et al., supra.

Example 3 Prophetic Cloning and Expression of Butanediol Dehydrogenase

The purpose of this prophetic Example is to describe how to clone and express in E. coli the budC gene that encodes the enzyme butanediol dehydrogenase. The budC gene is amplified from Klebsiella pneumoniae strain IAM1063 genomic DNA using PCR.

The budC sequence encoding butanediol dehydrogenase is cloned and expressed in the same manner as described for budA in Example 1, except that the primers used for PCR amplification are B5 (SEQ ID NO:19) and B6 (SEQ ID NO:20) and the genomic template DNA is from Klebsiella. pneumoniae IAM1063 (which is obtained from the Institute of Applied Microbiology Culture Collection, Tokyo, Japan). Klebsiella pneumoniae IAM1063 genomic DNA is prepared using the Gentra Puregene Puregene kit (Gentra Systems, Inc., Minneapolis, Minn.; catalog number D-5000A). The nucleotide sequence of the open reading frame (ORF) and the predicted amino acid sequence of the enzyme are given as SEQ ID NO:5 and SEQ ID NO:6, respectively.

Butanediol dehydrogenase activity in the cell free extracts is measured spectrophotometrically by following NADH consumption at an absorbance of 340 nm.

Example 4 Prophetic Cloning and Expression of Butanediol Dehydratase

The purpose of this prophetic Example is to describe how to clone and express in E. Coli the pddA, pddB and pddC genes that encode butanediol dehydratase. The pddA, pddB and pddC genes are amplified from Klebsiella oxytoca ATCC 8724 genomic DNA using PCR.

The pddA, pddB and pddC sequences which encode butanediol dehydratase are cloned and expressed in the same manner as described for budA in Example 1, except that the genomic template DNA is from Klebsiella oxytoca ATCC 8724, and the primers are B7 (SEQ ID NO:21) and B8 (SEQ ID NO:22). Klebsiella oxytoca genomic DNA is prepared using the Gentra Puregene Puregene kit (Gentra Systems, Inc., Minneapolis, Minn.; catalog number D-5000A). A single PCR product including all three open reading frames (ORFs) is cloned, so that all three coding regions are expressed as an operon from a single promoter on the expression plasmid. The nucleotide sequences of the open reading frames for the three subunits are given as SEQ ID NOs:7, 9, and 11, respectively, and the predicted amino acid sequences of the three enzyme subunits are given as SEQ ID NOs:8, 10, and 12, respectively.

Butanediol dehydratase activity in the cell free extracts is measured by derivatizing the ketone product with 2,4-dinitrophenylhydrazine (DNPH). Briefly, 100 μL of reaction mixture, cell extract containing approximately 0.0005 units of enzyme, 40 mM potassium phosphate buffer (pH 8.0), 2 μg of adenosylcobalamin, 5 μg of 2,3,-butanediol, and 1 μg of bovine serum albumin, is quenched by addition of an equal volume of 0.05 wt % DNPH in 1.0 N HCl. After 15 min at room temperature, the color is developed by addition of 100 μL of 4 N NaOH. The amount of product is determined from the absorbance of the final solution at 550 nm compared to a standard curve prepared with 2-butanone. All reactions are carried out at 37° C. under dim red light.

Example 5 Prophetic Cloning and Expression of Butanol Dehydrogenase

The purpose of this prophetic Example is to describe how to clone and express in E. coli the sadh gene that encodes butanol dehydrogenase. The sadh gene is amplified from Rhodococcus ruber strain 219 genomic DNA using PCR.

The sadh sequence encoding butanol dehydrogenase is cloned and expressed in the same manner as described for budA in Example 1, except that the genomic template DNA is from Rhodococcus ruber strain 219 (Meens, Institut fuer Mikrobiologie, Universitaet Hannover, Hannover, Germany) and the primers are B9 (SEQ ID NO:23) and B10 (SEQ ID NO:24). Rhodococcus ruber genomic DNA is prepared using the Ultra Clean™ Microbial DNA Isolation Kit (MO BIO Laboratories Inc., Carlsbad, Calif.), according to the manufacturer's protocol. The nucleotide sequence of the open reading frame (ORF) and the predicted amino acid sequence of the enzyme are given as SEQ ID NO:13 and SEQ ID NO:14, respectively.

Butanol dehydrogenase activity in cell free extracts is measured by following the increase in absorbance at 340 nm resulting from the conversion of NAD to NADH when the enzyme is incubated with NAD and 2-butanol.

Example 6 Prophetic Construction of a Transformation Vector for the Genes in a 2-Butanol Biosynthetic Pathway

The purpose of this prophetic Example is to describe the preparation of a transformation vector for the genes in a 2-butanol biosynthetic pathway (i.e., Pathway 3 as described above). Like most organisms, E. coli converts glucose initially to pyruvic acid. The enzymes required to convert pyruvic acid to 2-butanol following Pathway 3, i.e., acetolactate synthase, acetolactate decarboxylase, butanediol dehydrogenase, butanediol dehydratase, and butanol dehydrogenase, are encoded by the budA, budB, budC, pddA, pddB, pddC and sadh genes. To simplify building the 2-butanol biosynthetic pathway in a recombinant organism, the genes encoding the 5 steps in the pathway are divided into two operons. The upper pathway comprises the first three steps catalyzed by acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. The lower pathway comprises the last two steps catalyzed by butanediol dehydratase and butanol dehydrogenase.

The coding sequences are amplified by PCR with primers that incorporate restriction sites for later cloning, and the forward primers contain an optimized E. coli ribosome binding site (AAAGGAGG). PCR products are TOPO cloned into the pCR4Blunt-TOPO vector and transformed into Top10 cells (Invitrogen). Plasmid DNA is prepared from the TOPO clones, and the sequence of the cloned PCR fragment is verified. Restriction enzymes and T4 DNA ligase (New England Biolabs, Beverly, Mass.) are used according to manufacturer's recommendations. For cloning experiments, restriction fragments are gel-purified using QIAquick Gel Extraction kit (Qiagen).

After confirmation of the sequence, the coding regions are subcloned into a modified pUC19 vector as a cloning platform. The pUC19 vector is modified by a HindIII/SapI digest, followed by treatment with Klenow DNA polymerase to fill in the ends. The 2.4 kB vector fragment is gel-purified and religated creating pUC19dHS. Alternatively the pUC19 vector is modified by a SphI/SapI digest, followed by treatment with Klenow DNA polymerase to blunt the ends. The 2.4 kB vector fragment is gel-purified and religated creating pUC19dSS. The digests remove the lac promoter adjacent to the MCS (multiple cloning sites), preventing transcription of the operons from the vector.

Upper Pathway:

The budABC coding regions are amplified from Klebsiella pneumoniae genomic DNA by PCR using primer pair B11 and B12 (Table 4), given as SEQ ID NOs:25 and 26, respectively. The forward primer incorporates an EcoRI restriction site and a ribosome binding site (RBS). The reverse primer incorporates an SphI restriction site. The PCR product is cloned into pCR4 Blunt-TOPO creating pCR4 Blunt-TOPO-budABC.

To construct the upper pathway operon pCR4 Blunt-TOPO-budABC is digested with EcoRI and SphI releasing a 3.2 kbp budABC fragment. The pUC19dSS vector is also digested with EcoRI and SphI, releasing a 2.0 kbp vector fragment. The budABC fragment and the vector fragment are ligated together using T4 DNA ligase (New England Biolabs) to form pUC19dSS-budABC.

Lower Pathway:

The pddABC coding regions are amplified from Klebsiella oxytoca ATCC 8724 genomic DNA by PCR using primers B13 and B14 (Table 4), given as SEQ ID NOs:27 and 28, respectively, creating a 2.9 kbp product. The forward primer incorporates EcoRI and PmeI restriction sites and a RBS. The reverse primer incorporates the BamHI restriction site. The PCR product is cloned into pCRBlunt II-TOPO creating pCRBluntII-pdd.

The sadh gene is amplified from Rhodococcus ruber strain 219 genomic DNA by PCR using primers B15 and B16 (Table 4), given as SEQ ID NOs:29 and 30, respectively, creating a 1.0 kbp product. The forward primer incorporates a BamHI restriction site and a RBS. The reverse primer incorporates an XbaI restriction site. The PCR product is cloned into pCRBlunt II-TOPO creating pCRBluntII-sadh.

To construct the lower pathway operon, a 2.9 kbp EcoRI and BamHI fragment from pCRBluntII-pdd, a 1.0 kbp BamHI and XbaI fragment from pCRBluntII-sadh, and the large fragment from an EcoRI and XbaI digest of pUC19dHS are ligated together. The three-way ligation creates pUC19dHS-pdd-sadh.

The pUC19dSS-budABC vector is digested with PmeI and HindIII, releasing a 3.2 kbp fragment that is cloned into pBenBP, an E. coli-B. subtilis shuttle vector. Plasmid pBenBP is created by modification of the pBE93 vector, which is described by Nagarajan (WO 93/2463, Example 4). To generate pBenBP, the Bacillus amyloliquefaciens neutral protease promoter (NPR) signal sequence and the phoA gene are removed from pBE93 with an NcoI/HindIII digest. The NPR promoter is PCR amplified from pBE93 by primers BenF and BenBPR, given by SEQ ID NOs:31 and 32, respectively. Primer BenBPR incorporates BstEII, PmeI and HindIII sites downstream of the promoter. The PCR product is digested with NcoI and HindIII, and the fragment is cloned into the corresponding sites in the vector pBE93 to create pBenBP. The upper operon fragment is subcloned into the PmeI and HindIII sites in pBenBP creating pBen-budABC.

The pUC19dHS-pdd-sadh vector is digested with PmeI and HindIII releasing a 3.9 kbp fragment that is cloned into the PmeI and HindIII sites of pBenBP, creating pBen-pdd-sadh.

Example 7 Prophetic Expression of a 2-Butanol Biosynthetic Pathway in E. coli

The purpose of this prophetic Example is to describe how to express a 2-butanol biosynthetic pathway in E. coli.

The plasmids pBen-budABC and pBen-pdd-sadh, prepared as described in Example 6, are separately transformed into E. coli NM522 (ATCC No. 47000), and expression of the genes in each operon is monitored by SDS-PAGE analysis and enzyme assay. After confirmation of expression of all genes, pBen-budABC is digested with EcoRI and HindIII to release the NPR promoter-budABC fragment. The fragment is blunt ended using the Klenow fragment of DNA polymerase (New England Biolabs, catalog no. M0210S). The plasmid pBen-pdd-sadh is digested with EcoRI and similarly blunted to create a linearized, blunt-ended vector fragment. The vector and NPR-budABC fragments are ligated, creating p2BOH. This plasmid is transformed into E. coli NM522 to give E. coli NM522/p2BOH, and expression of the genes is monitored as previously described.

E. coli NM522/p2BOH is inoculated into a 250 mL shake flask containing 50 mL of medium and shaken at 250 rpm and 35° C. The medium is composed of: dextrose, 5 g/L; MOPS, 0.05 M; ammonium sulfate, 0.01 M; potassium phosphate, monobasic, 0.005 M; S10 metal mix, 1% (v/v); yeast extract, 0.1% (w/v); casamino acids, 0.1% (w/v); thiamine, 0.1 mg/L; proline, 0.05 mg/L; and biotin 0.002 mg/L, and is titrated to pH 7.0 with KOH. S10 metal mix contains: MgCl₂, 200 mM; CaCl₂, 70 mM; MnCl₂, 5 mM; FeCl₃, 0.1 mM; ZnCl₂, 0.1 mM; thiamine hydrochloride, 0.2 mM; CuSO₄, 172 μM; CoCl₂, 253 μM; and Na₂MoO₄, 242 μM. After 18 h, 2-butanol is detected by HPLC or GC analysis using methods that are well known in the art, for example, as described in the General Methods section above.

Example 8 Prophetic Expression of a 2-Butanol Biosynthetic Pathway in Bacillus subtilis

The purpose of this prophetic Example is to describe how to express a 2-butanol biosynthetic pathway in Bacillus subtilis.

The plasmids pBen-budABC and pBen-pdd-sadh, prepared as described in Example 6, are separately transformed into Bacillus subtilis BE1010 (J. Bacteriol. 173:2278-2282 (1991)) and expression of the genes in each operon is monitored as described in Example 7. The plasmid pBen-budABC is digested with EcoRI and HindIII to release the NPR promoter-budABC fragment. The fragment is blunt ended using the Klenow fragment of DNA polymerase (New England Biolabs, catalog no. M0210S). The plasmid pBen-pdd-sadh is digested with EcoRI and similarly blunted to create a linearized, blunt-ended vector fragment. The vector and NPR-budABC fragments are ligated, creating p2BOH. This plasmid is transformed into Bacillus subtilis BE1010 to give Bacillus subtilis BE1010/p2BOH, and expression of the genes is monitored as previously described.

Bacillus subtilis BEl010/p2BOH is inoculated into a 250 mL shake flask containing 50 mL of medium and shaken at 250 rpm and 35° C. for 18 h. The medium is composed of: dextrose, 5 g/L; MOPS, 0.05 M; glutamic acid, 0.02 M; ammonium sulfate, 0.01 M; potassium phosphate, monobasic buffer, 0.005 M; S10 metal mix (as described in Example 7), 1% (v/v); yeast extract, 0.1% (w/v); casamino acids, 0.1% (w/v); tryptophan, 50 mg/L; methionine, 50 mg/L; and lysine, 50 mg/L, and is titrated to pH 7.0 with KOH. After 18 h, 2-butanol is detected by HPLC or GC analysis using methods that are well known in the art, for example, as described in the General Methods section above.

Example 9 Construction of a Transformation Vector for the Genes in a 2-Butanol Biosynthetic Pathway

The purpose of this Example was to prepare a recombinant E. coli host carrying the genes in a 2-butanol biosynthetic pathway (i.e., Pathway 3 as described above). Like most organisms, E. coli converts glucose initially to pyruvic acid. The enzymes required to convert pyruvic acid to 2-butanone in Pathway 3, i.e., acetolactate synthase, acetolactate decarboxylase, butanediol dehydrogenase, and butanediol dehydratase are encoded by the budA, budB, budC, pddA, pddB, and pddC genes. In the last step of the pathway, a butanol dehydrogenase converts 2-butanone to 2-butanol. Dehydrogenases that carry out this last step are promiscuous and may be found in many organisms. To simplify building the 2-butanol biosynthetic pathway in a recombinant organism, the genes encoding the 5 steps in the pathway were divided into multiple operons. The upper pathway operon comprised the first three steps catalyzed by acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase and were cloned onto an expression vector. The lower pathway comprised the last two steps catalyzed by butanediol dehydratase including the reactivating factor (Mori et al., J. Biol. Chem. 272:32034 (1997)) and a butanol dehydrogenase. The diol dehydratase can undergo suicide inactivation during catalysis. The reactivating factor protein encoded by ddrA and ddrB (GenBank AF017781, SEQ ID NO:70) reactivates the inactive enzyme. The ddrA and ddrB genes flank the diol dehydratase operon. The operons for the dehydratase/reactivating factor and the butanol dehydrogenase were either cloned onto another expression vector or the dehydratase/reactivating factor operon was cloned singly onto another expression vector and the last step was provided by an endogenous activity in the demonstration host.

Construction of Vector pTrc99a-budABC:

The budAB coding regions were amplified from K. pneumoniae ATCC 25955 genomic DNA by PCR using primer pair BABC F and BAB R, given as SEQ ID NOs:33 and 34, respectively (see Table 4), creating a 2.5 kbp product. The forward primer incorporated SacI and EcoRI restriction sites and a ribosome binding site (RBS). The reverse primer incorporated a SpeI restriction site. The PCR product was cloned into pCR4 Blunt-TOPO creating pCR4 Blunt-TOPO-budAB. Plasmid DNA was prepared from the TOPO clones and the sequence of the genes was verified with primers M13 Forward (SEQ ID NO:35), M13 Reverse (SEQ ID NO:36), N83 SeqF2 (SEQ ID NO:37), N83 SeqF3 (SEQ ID NO:38) and N84 SeqR4 (SEQ ID NO:39) (see Table 5).

The budC coding region was amplified from K. pneumoniae ATCC 25955 genomic DNA by PCR using primer pair BC Spe F and BC Xba R given as SEQ ID NOs:40 and 41, respectively, creating a 0.8 kbp product. The forward primer incorporated a SpeI restriction site, a RBS and modified the CDS by changing the second and third codons from AAA to AAG. The reverse primer incorporated an XbaI restriction site. The PCR product was cloned into pCR4 Blunt-TOPO creating pCR4 Blunt-TOPO-budC. Plasmid DNA was prepared from the TOPO clones and the sequence of the genes was verified with primers M13 Forward (SEQ ID NO:35) and M13 Reverse (SEQ ID NO:36).

To construct the budABC operon, pCR4 Blunt-TOPO-budC was digested with SnaBI and XbaI releasing a 1.0 kbp budC fragment. The vector pTrc99a (Amann et al., Gene 69(2):301-315 (1988)) was digested with SmaI and XbaI creating a 4.2 kbp linearized vector fragment. The vector and the budC fragment were ligated to create pTrc99a-budC and transformed into E. coli Top 10 cells (Invitrogen). Transformants were analyzed by PCR amplification with primers Trc F (SEQ ID NO:42) and Trc R (SEQ ID NO:43) for a 1.2 kbp product to confirm the presence of the budC insert. The budAB genes were subcloned from pCR4 Blunt-TOPO-budAB as a 2.5 kbp EcoRI/SpeI fragment. Vector pTrc99a-budC was digested with EcoRI and SpeI and the resulting 5.0 kbp vector fragment was gel-purified. The purified vector and budAB insert were ligated and transformed into E. coli Top 10 cells. Transformants were screened by PCR amplification with primers Trc F (SEQ ID NO:42) and N84 Seq R2 (SEQ ID NO:65) to confirm creation of pTrc99a-budABC. In this plasmid, the bud A, B, and C coding regions are adjacent to each other, in this order, and between the Trc promoter and the rrnB termination sequence.

Results:

Three independent isolates of E. coli Top 10/pTrc99a-budABC were examined for the production of butanediol, using E. coli Top 10/pCL1925-Kodd-ddr (described below) as a negative control. The strains were grown in LB medium containing 100 μg/mL carbenicillin. The resulting cells were used to inoculate shake flasks (approximately 175 mL total volume) containing 125 mL of TM3a/glucose medium with 100 μg/mL carbenicillin. In addition, the flasks inoculated with strains carrying pTrc99a-budABC contained 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). TM3a/glucose medium contains (per liter): 10 g glucose, 13.6 g KH₂PO₄, 2.0 g citric acid monohydrate, 3.0 g (NH₄)₂SO₄, 2.0 g MgSO₄.7H₂O, 0.2 g CaCl₂.2H₂O, 0.33 g ferric ammonium citrate, 1.0 mg thiamine HCl, 0.50 g yeast extract, and 10 mL trace elements solution, adjusted to pH 6.8 with NH₄OH. The solution of trace elements contained: citric acid H₂O (4.0 g/L), MnSO₄.H₂O (3.0 g/L), NaCl (1.0 g/L), FeSO₄.7H₂O (0.10 g/L), CoCl₂.6H₂O (0.10 g/L), ZnSO₄.7H₂O (0.10 g/L), CuSO₄.5H₂O (0.010 g/L), H₃BO₃ (0.010 g/L), and Na₂MoO₄ 2H₂O (0.010 g/L). The flasks, capped with vented caps, were inoculated at a starting OD₆₀₀ of approximately 0.03 units and incubated at 34° C. with shaking at 300 rpm.

Approximately 23 h after induction, an aliquot of the broth was analyzed by HPLC (Shodex Sugar SH1011 column) and GC (HP-INNOWax), using the same methods described in the General Methods section for 2-butanol and 2-butanone. The results of the analysis are given in Table 6. The three E. coli clones converted glucose to acetoin and meso-2,3-butanediol, the desired intermediates of the pathway, with a molar selectivity of 14%. This selectivity was approximately 35-fold higher than that observed with the E. coli control strain lacking budABC.

TABLE 6 Production of Acetoin and meso-2,3-butanediol by E. coli Top 10/pTrc99a-budABC Meso-2,3- Butanediol, Molar Strain OD₆₀₀ Acetoin, mM mM Selectivity^(a), % Negative 1.4 0.07 0.03 0.4 control Isolate #1 1.5 0.64 1.3 14 Isolate #2 1.4 0.70 1.2 14 Isolate #3 1.4 0.74 1.3 15 ^(a)Molar selectivity is (acetoin + meso-2,3-butanendiol)/(glucose consumed). Construction of Vector pCL1925-KoDD-ddr:

The diol dehydratase (GenBank D45071, SEQ ID NO:69) and reactivating factor (GenBank AF017781, SEQ ID NO:70) operons were PCR amplified from Klebsiella oxytoca ATCC 8724 as a single unit with primers DDo For (SEQ ID NO: 44) and DDo Rev (SEQ ID NO:45). The forward primer incorporated an optimized E. coli RBS and a HindIII restriction site. The reverse primer included an XbaI restriction site. The 5318 bp PCR product was cloned into pCR4Blunt-TOPO and clones of the resulting pCR4Blunt-TOPO-Kodd-ddr were sequenced with primers M13 Forward (SEQ ID NO:35), M13 Reverse (SEQ ID NO:36), DDko seq F2 (SEQ ID NO:46), DDko seq F5 (SEQ ID NO:47), DDko seq F7 (SEQ ID NO:48), DDko seq F9 (SEQ ID NO:49), DDko seq R1 (SEQ ID NO:50), DDko seq R3 (SEQ ID NO:51), DDko seq R7 (SEQ ID NO:52), and DDko seq R10 (SEQ ID NO:53). A clone having the insert with the expected sequence was identified.

For expression, the diol dehydratase/reactivating factor genes were subcloned into pCL1925 (U.S. Pat. No. 7,074,608), a low copy plasmid carrying the glucose isomerase promoter from Streptomyces. pCR4Blunt-TOPO-Kodd-ddr was digested with HindIII and XbaI and the resulting 5.3 kbp Kodd-ddr fragment was gel-purified. Vector pCL1925 was digested with HindIII and XbaI and the resulting 4539 bp vector fragment was gel purified. The vector and Kodd-ddr fragment were ligated and transformed into E. coli Top10. Transformants were screened by PCR with primers DDko Seq F7 (SEQ ID NO:48) and DDko Seq R7 (SEQ ID NO: 52). Amplification of the plasmid (pCL1925-Kodd-ddr) carrying the insert resulted in a product of approximately 797 bp.

Activity of diol dehydratase towards meso-2,3-butanediol was measured by incubating cell extract (total protein ˜0.8 mg/mL) with 10 mM butanediol and 12 mM coenzyme B₁₂ in 80 mM HEPES (pH 8.2) for 17 h at room temperature. Formation of the expected product, 2-butanone, was determined by HPLC as described in the General Methods.

Construction of Vector pCL1925-KoDD-ddr::T5 chnA ter:

To provide a heterologous alcohol dehydrogenase activity, the chnA gene encoding cyclohexanol dehydrogenase from Acinetobacter sp. (Cheng et al., J. Bacteriol. 182:4744-4751 (2000)) was cloned into the pCL1925 vector with the diol dehydratase operon, pCL1925-Kodd-ddr. The chnA gene, given as SEQ ID NO:71 (Genbank No: AF282240, SEQ ID NO:73) was amplified from pDCQ2, a cosmid carrying the cyclohexanol gene cluster from Acinetobacter, with primers ChnA F (SEQ ID NO:54) and ChnA R (SEQ ID NO:55). The resulting 828 bp PCR product was cloned into pCR4Blunt-TOPO to create pCR4Blunt-TOPO-chnA and transformants were screened by colony PCR with primers M13 Forward (SEQ ID NO:35) and M13 Reverse (SEQ ID NO:36). Correct clones produced a PCR product of about 1 kbp and were sequenced with primers M13 Forward (SEQ ID NO:35) and M13 Reverse (SEQ ID NO:36).

After sequencing pCR4Blunt-TOPO-chnA to confirm the correct sequence, the chnA gene was subcloned from the plasmid as an 813 bp MfeI/SmaI fragment. The expression vector pQE30 (Qiagen) was digested with MfeI and SmaI and the resulting 3350 bp vector fragment was gel-purified. The chnA fragment and the purified vector were ligated and transformed into E. coli Top10 cells. Transformants were colony PCR screened with primers chnSeq F1 (SEQ ID NO:56) and chnseq R1 (SEQ ID NO:57) for a 494 bp PCR product. This cloning placed the chnA gene under the control of the T5 promoter in the plasmid, pQE30-chnA.

To prepare the pCL1925 vector to carry two operons, terminators were added to the vector. A tonB terminator-mcs-trpA terminator fragment was prepared by oligonucleotide annealing with primers Top ter F1 (SEQ ID NO:58), Top ter F2 (SEQ ID NO:59), Bot ter R1 (SEQ ID NO:60) and Bot ter R2 (SEQ ID NO:61). The annealed DNA was gel-purified on a 6% PAGE gel (Embi-tec, San Diego, Calif.). Vector pCL1925 was digested with SacI and XbaI and gel-purified. The annealed DNA and vector fragment were ligated to create pCL1925-ter. Transformants were screened by colony PCR amplification with primers pCL1925 vec F (SEQ ID NO:62) and pCL1925 vec R1 (SEQ ID NO:63) for the presence of a PCR product of approximately 400 bp. Positive clones from the PCR screen were sequenced with the same primers.

Vector pCL1925-ter was digested with XhoI and PmeI and the resulting 4622 bp fragment was gel-purified. pQE30-chnA was digested with NcoI and the DNA was treated with Klenow DNA polymerase to blunt the ends. pQE30-chnA was then digested with XhoI and the resulting 1.2 kbp T5 promoter-chnA fragment was gel-purified. The pCL1925-ter vector and the chnA operon fragment were ligated together to give pCL1925-ter-T5chnA and transformed into E. coli Top10. Transformants were screened by colony PCR amplification with primers pCL1925 vec F (SEQ ID NO:64) and chnseq R1 (SEQ ID NO:59) for a product of approximately 1 kbp.

To finish building the pathway vector, the pCL1925-KoDD-ddr plasmid was digested with XbaI and SacI and the resulting 9504 bp vector fragment was gel-purified. The chnA operon flanked by terminators, with the trpA terminator (Koichi et al. (1997) Volume 272, Number 51, pp. 32034-32041) 3′ to the chnA coding sequence, from pCL1925-ter-T5chnA was gel-purified as a 1271 bp XbaI/SacI fragment. After ligation of the fragments and transformation into E. coli Top10, transformants were screened by colony PCR. Primers chnSeq F1 (SEQ ID NO:58) and pCL1925 vec R2 (SEQ ID NO:64) amplified the expected 1107 bp PCR product in the resulting plasmid, pCL1925-KoDD-ddr::ter-T5chnA.

Example 10 Expression of a 2-Butanol Biosynthetic Pathway in E. coli with Overexpressed Endogenous Alcohol Dehydrogenase

The purpose of this Example was to express a 2-butanol biosynthetic pathway in several E. coli strains.

Construction of E. coli Strains Constitutively Expressing yqhD:

E. coli contains a native gene (yqhD) that was identified as a 1,3-propanediol dehydrogenase (U.S. Pat. No. 6,514,733). The yqhD gene, given as SEQ ID NO:74, has 40% identity to the gene adhB in Clostridium, a probable NADH-dependent butanol dehydrogenase. The yqhD gene was placed under the constitutive expression of a variant of the glucose isomerase promoter 1.6GI (SEQ ID NO:67) in E. coli strain MG1655 1.6yqhD::Cm (WO 2004/033646) using X Red technology (Datsenko and Wanner, Proc. Natl. Acad. Sci. U.S.A. 97:6640 (2000)). Similarly, the native promoter was replaced by the 1.5GI promoter (WO 2003/089621) (SEQ ID NO:68), creating strain MG1655 1.5yqhD::Cm, thus, replacing the 1.6GI promoter of MG1655 1.6yqhD::Cm with the 1.5GI promoter. The 1.5GI and 1.6GI promoters differ by 1 bp in the −35 region, thereby altering the strength of the promoters (WO 2004/033646). While replacing the native yqhD promoter with either the 1.5GI or 1.6GI promoter, the yqhC gene encoding the putative transcriptional regulator for the yqh operon was deleted. Butanol dehydrogenase activity was confirmed by enzyme assay using methods that are well known in the art.

Transformation of E. coli Strains:

Pathway plasmids pCL1925-Kodd-ddr and pTrc99a-budABC, described in Example 9, were co-transformed into E. coli strains MG1655, MG1655 1.6yqhD, and MG1655 1.5yqhD. The two latter strains overexpress the 1,3-propanediol dehydrogenase, YqhD, which also has butanol dehydrogenase activity. Strains were examined for the production of 2-butanone and 2-butanol essentially as described above. Cells were inoculated into shake flasks (approximately 175 mL total volume) containing either 50 or 150 mL of TM3a/glucose medium (with 0.1 mg/L vitamin B₁₂, appropriate antibiotics and IPTG) to represent medium and low oxygen conditions, respectively. Spectinomycin (50 μg/mL) and carbenicillin (100 μg/mL) were used for plasmids pCL1925-Kodd-ddr and pTrc99a-budABC, respectively. The flasks were inoculated at a starting OD₆₀₀ of <0.04 units and incubated at 34° C. with shaking at 300 rpm. The flasks containing 50 mL of medium were capped with vented caps; the flasks containing 150 mL, were capped with non-vented caps to minimize air exchange. IPTG was present at time zero at a concentration of zero or 0.04 mM. Analytical results for 2-butanone and 2-butanol production are presented in Table 7. All the E. coli strains comprising a 2-butanol biosynthetic pathway produced 2-butanone under low and medium oxygen conditions and produced 2-butanol under low oxygen conditions.

TABLE 7 Production of 2-Butanone and 2-Butanol by E. coli MG1655 strains harboring pathway plasmids pCL1925-Kodd-ddr and pTrc99a-budABC Volume of 2-Butanone, 2-Butanol, Strain^(a,b) IPTG, mM Medium, mL mM mM MG1655 #1 0 50 0.08 Not detected MG1655 #2 0 50 0.11 Not detected MG1655 #1 0.04 50 0.12 Not detected MG1655 #2 0.04 50 0.11 Not detected MG1655 #1 0 150 0.15 0.047 MG1655 #2 0 150 0.19 0.041 MG1655 #1 0.04 150 0.10 0.015 MG1655 #2 0.04 150 0.11 0.015 MG1655 0 50 0.10 Not detected 1.5yqhD #1 MG1655 0 50 0.07 Not detected 1.5yqhD #2 MG1655 0.04 50 0.12 Not detected 1.5yqhD #1 MG1655 0.04 50 0.18 Not detected 1.5yqhD #2 MG1655 0 150 0.16 0.030 1.5yqhD #1 MG1655 0 150 0.18 0.038 1.5yqhD #2 MG1655 0.04 150 0.10 0.021 1.5yqhD #1 MG1655 0.04 150 0.09 0.017 1.5yqhD #2 MG1655 0 50 0.08 Not detected 1.6yqhD #1 MG1655 0 50 0.07 Not detected 1.6yqhD #2 MG1655 0.04 50 0.12 Not detected 1.6yqhD #1 MG1655 0.04 50 0.15 Not detected 1.6yqhD #2 MG1655 0 150 0.17 0.019 1.6yqhD #1 MG1655 0 150 0.18 0.041 1.6yqhD #2 MG1655 0.04 150 0.11 0.026 1.6yqhD #1 MG1655 0.04 150 0.11 0.038 1.6yqhD #2 Control Not detected Not detected (uninoculated medium) ^(a)#1 and #2 represent independent isolates. ^(b)MG1655 is MG1655/pCL1925-Kodd-ddr/pTrc99a-budABC MG1655 1.6yqhD is MG1655 1.6yqhD/pCL1925-Kodd-ddr/pTrc99a-budABC MG1655 1.6yqhD is MG1655 1.5yqhD/pCL1925-Kodd-ddr/pTrc99a-budABC.

Example 11 Expression of a 2-Butanol Biosynthetic Pathway in E. coli with Heterologous Alcohol Dehydrogenase

Plasmids pCL1925-KoDD-ddr::ter-T5chnA and pTrc99a-budABC, described in Example 9, were transformed into E. coli strains MG1655 and MG1655 ΔyqhCD for a demonstration of the production of 2-butanol.

MG1655 ΔyqhCD carries a yqhCD inactivation that was made using the method of Datsenko and Wanner (Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-6645 (2000)). After replacement of the region with the FRT-CmR-FRT cassette of pKD3, the chloramphenicol resistance marker was removed using the FLP recombinase. The sequence of the deleted region is given as SEQ ID NO:66.

Strains MG1655/pTrc99a-budABC/pCL1925KoDD-ddr::ter-T5 chnA and MG1655 ΔyqhCD/pTrc99a-budABC/pCL1925KoDD-ddr::ter-T5 chnA were examined for the production of 2-butanone and 2-butanol essentially as described above. Strain MG1655 ΔyqhCD/pCL1925 was used as a negative control. Cells were inoculated into shake flasks (approximately 175 mL total volume) containing 50 or 150 mL of TM3a/glucose medium (with 0.1 mg/L vitamin B₁₂ and appropriate antibiotics) to represent medium and low oxygen conditions, respectively. Spectinomycin (50 μg/mL) and ampicillin (100 μg/mL) were used for selection of pCL1925 based plasmids and pTrc99a-budABC, respectively. Enzyme activity derived from pTrc99a-budABC was detected by enzyme assay in the absence of IPTG inducer, thus, IPTG was not added to the medium. The flasks were inoculated at a starting OD₆₀₀ of <0.01 units and incubated at 34° C. with shaking at 300 rpm for 24 h. The flasks containing 50 mL of medium were capped with vented caps; the flasks containing 150 mL, were capped with non-vented caps to minimize air exchange. Analytical results for 2-butanone and 2-butanol production are presented in Table 8. Both E. coli strains comprising a 2-butanol biosynthetic pathway produced 2-butanone under low and medium oxygen conditions and produced 2-butanol under low oxygen conditions, while the negative control strain did not produce detectable levels of either 2-butanone or 2-butanol.

TABLE 8 Production of 2-butanone and 2-butanol by E. coli strains Vol- ume, 2-Butanone, 2-Butanol, Strain^(a) mL mM mM Negative control, MG1655 50 Not detected Not detected ΔyqhCD/pCL1925 MG1655/pTrc99a- 50 0.33 Not detected budABC/pCL1925KoDD-ddr::T5 chnA ter MG1655 ΔyqhCD/pTrc99a- 50 0.23 Not detected budABC/pCL1925KoDD-ddr::T5 chnA ter#1 MG1655 ΔyqhCD/pTrc99a- 50 0.19 Not detected budABC/pCL1925KoDD-ddr::T5 chnA #2 Negative control, MG1655 150 Not detected Not detected ΔyqhCD/pCL1925 MG1655/pTrc99a- 150 0.41 0.12 budABC/pCL1925KoDD-ddr::T5 chnA ter MG1655 ΔyqhCD/pTrc99a- 150 0.15 0.46 budABC/pCL1925KoDD-ddr::T5 chnA #1 MG1655 ΔyqhCD/pTrc99a- 150 0.44 0.14 budABC/pCL1925KoDD-ddr::T5 chnA #2 Medium Not detected Not detected ^(a)#1 and #2 represent independent isolates.

Example 12 Cloning of Amino:Pyruvate Transaminase (APT)

An amino:pyruvate transaminase (APT) from Vibrio Fluvialis JS17 was identified by Shin et al. (Appl. Microbiol. Biotechnol. (2003) 61:463-471). The amino acid sequence (SEQ ID NO:122) was found to have significant homology with co-amino acid:pyruvate transaminases (Shin and Kim (J. Org. Chem. 67:2848-2853 (2002)). It was shown that the Vibrio Fluvialis APT has transaminase activity towards acetoin.

For expression of the APT enzyme in E. coli, a codon optimized APT coding region (SEQ ID NO:144) was designed using the preferred E. coli codons with additional considerations such as codon balance and mRNA stability, and synthesized (by DNA2.0; Redwood City, Calif.). The coding region DNA fragment was subcloned into the pBAD.HisB vector (Invitrogen) between the NcoI and HindIII sites and the resulting plasmid, hereafter referred to as pBAD.APT1, was transformed into TOP10 cells.

Example 13 Characterization of Vibrio Fluvialis APT Alanine:Acetoin Aminotransferase Activity

A 5 mL volume of LB broth+100 μg/mL ampicillin was inoculated with a fresh colony of TOP10/pBAD:APT1 cells. The culture was incubated at 37° C. for approximately 16 h with shaking (225 rpm). A 300 μL aliquot of this culture was used to inoculate 300 mL of the same medium, which was incubated at 37° C. with shaking (225 rpm). When the culture reached an OD₆₀₀ of 0.8, L-arabinose was added to a final concentration of 0.2% (w/v). The culture was incubated for an additional 16 h, then harvested. The cells were washed once with 100 mM potassium phosphate buffer (pH 7.8) and then frozen and stored at −80° C.

To isolate the enzyme, the cell pellet was thawed and resuspended in 8 mL of 100 mM potassium phosphate buffer (pH 7) containing 0.2 mM ethylenediaminetetraacetate, 1 mM dithiothreitol and 1 tablet of protease inhibitor cocktail (Roche; Indianapolis, Ind.). The cells were lysed by two passes through a French pressure cell at 900 psi, and the resulting lysate was clarified by centrifugation for 30 min at 17000×g. Ammonium sulfate was added to 35% saturation, and the solution was stirred for 30 min at room temperature, at which point precipitated solids were removed by centrifugation (30 min, 17000×g). Additional ammonium sulfate was added to the supernatant to give 55% saturation, and the solution was again stirred for 30 min at room temperature. The precipitated solids were removed by centrifugation (30 min, 17000×g) and then resuspended in 5 mL of 100 mM potassium phosphate buffer (pH 7) containing 10 μM pyridoxal 5′-phosphate and 1 mM dithiothreitol. This solution was desalted by passage through a PD10 column equilibrated with Buffer A (50 mM bis-tris propane buffer (pH 6) containing 10 μM pyridoxal 5′-phosphate and 1 mM dithiothreitol). The desalted extract was then loaded onto a 20 mL Q-Fast Flow column pre-equilibrated with Buffer A. APT was eluted with a linear gradient of 0-0.1 M NaCl in Buffer A. The enzyme was detected in eluted fractions by the presence of a protein band of size ˜50 kD when analyzed by SDS-polyacrylamide gel electrophoresis and by the characteristic absorbance at 418 nm. Fractions containing the enzyme eluted at ˜0.3 M NaCl. These fractions were pooled to yield a total of 6 mL of a 5.45 mg/mL solution of enzyme, which was >90% pure, as judged by SDS-polyacrylamide gel electrophoresis.

The alanine:acetoin aminotransferase activity of APT wasassayed using a lactic dehydrogenase coupled assay. Reaction mixtures contained 100 mM bis-tris propane (pH 9.0), 10 μM pyridoxal 5′-phosphate, 0-50 mM acetoin, 0-5 mM L-alanine, 0.14 or 0.28 mg/mL purified enzyme, 200 μM NADH and 20 U/mL lactic dehydrogenase (Sigma; St. Louis, Mo.). The reaction was followed by measuring the change in absorbance at 340 nm, indicative of the oxidation of NADH. Under these conditions, the k_(cat)/K_(m) for acetoin was 10 M⁻¹ s⁻¹ and that for L-alanine was 400 M⁻¹ s⁻¹.

The identity of the expected product 3-amino-2-butanol was confirmed by comparison to a synthetic standard. A mixture of (R,R)- and (S,S)-3-amino-2-butanol was synthesized by the method of Dickey et al. [J Amer Chem Soc 74:944 (1952)]: 5 g of trans-2,3-epoxybutane were slowly stirred into 150 mL of cold (4° C.) NH₄OH. The reaction was slowly warmed to room temperature, sealed and stirred at room temperature for an additional 10 days. At this time, excess ammonia and water and residual epoxybutane were removed by rotary evaporation under vacuum at 40° C. The resulting clear oil (2.9 g) was resuspended in water to a concentration of 10% (w/v). Production of the desired product was confirmed by NMR analysis and comparison of the spectrum to that reported by Levy et al. [Org. Magnetic Resonance 14:214 (1980)]. A mixture of the corresponding (2R,3S)- and (2S,3R)-isomers was produced using the identical method with the exception that the starting material was the cis-isomer of 2,3-epoxybutane.

An analytical method for detection of 3-amino-2-butanol was developed based on the o-phthaldialdehyde derivatization method for amino acid determination reported by Roth [Anal. Chem. 43:880 (1971)]. A 200 μL aliquot of 1 mM 3-amino-2-butanol (mixture of isomers) was mixed with 200 μL of a 50 mM solution of borate (pH 9.5), to which was added 10 μL of 5 μL/mL 2-mercaptoethanol in ethanol and 10 μL of 10 mg/mL o-phthaldialdehdye in ethanol. The solution was incubated at room temperature for 10 min, at which time the derivative was extracted into 200 μL hexane. The hexane was separated from the aqueous solution by decanting, and 10 μL were injected onto a Chiracel OD HPLC column (Daicel Chemical Industries; Fort Lee, N.J.). The column was run isocratically with a mobile phase of 90:10 hexane:isopropanol at a rate of 1 mL/min. The derivatized isomers of 3-amino-2-butanol were detected by absorbance at 340 nm with retention times of approximately 15.7 and 16.8 min [(2S,3S) and (2R,3R)], and 18.4 and 21.9 min [(2R,3S) and (2S,3R)]. To differentiate the enantiomers in the first mixture, the pure (2R,3R) isomer (Bridge Organics; Vicksburg, Mich.) was also run under the identical conditions and found to be the 16.8 min peak. To differentiate the enantiomers in the second mixture, the mixture was first kinetically resolved using the alanine:acetoin aminotransferase: 0.28 mg of purified enzyme was incubated with 10 mM pyruvate and 10 mM 3-amino-2-butanol [1:1 mixture of (2R,3S) and (2S,3R) isomers] in 1 mL of 100 mM bis-tris propane (pH 9.0). After 24 h at room temperature, an aliquot was removed and analyzed as described above. Analysis revealed that the 18.4 min peak was 95% depleted, while the 21.9 min peak was >90% retained. A 100 μL aliquot of the remaining reaction mixture was mixed with 50 μL of 20 mM NADH and 10 μL of extract from the TOP10/pTrc99a-BudC strain described in Example 9. The BudC enzyme is known to reduce (R)-acetoin to meso-2,3-butanediol and (S)-acetoin to (S,S)-2,3-butanediol [Ui et al., (2004) Letters in Applied Microbiology 39:533-537]. After 3 h, samples were taken from the reaction and analyzed as described above for acetoin and butanediol. The analysis indicated that the primary product of the reduction was meso-2,3-butanediol, indicating that the product of the aminotransferase reaction was (R)-acetoin, and therefore the consumed 3-amino-2-butanol isomer was the (2R,3S) isomer. Thus the retention time of 18.4 min can be assigned to this isomer and 21.9 to the (2S,3R) isomer.

To confirm that the product of the APT-catalyzed alanine:acetoin aminotransferase reaction was 3-amino-2-butanol, 0.28 mg of purified enzyme was incubated with 10 mM acetoin, 10 mM L-alanine, 50 U lactic dehydrogenase and 200 μM NADH in 1 mL of 100 mM bis-tris propane (pH 9.0). The reaction mixture was incubated at room temperature for 20 h, after which a 200 μL aliquot was removed and derivatized as described above. The retention times of the derivatized products were 15.8 min (major product) and 18.5 min (minor product), matching that of the (2S,3S)- and (2R,3S)-3-amino-2-butanol standards.

Example 14 Identification and Cloning of Erwinia carotovora Subsp. atroseptica Amino Alcohol Kinase and Amino Alcohol O-Phosphate Lyase

The purpose of this example is to describe the identification and cloning of sequences encoding an amino alcohol kinase and amino alcohol O-phosphate lyase from the bacterium Erwinia carotovora. These two enzymes are part of Pathway 1 for the conversion of 3-amino-2-butanol to 2-butanone via the intermediate 3-amino-2-butanol phosphate as shown in FIG. 1.

Prediction of the Erwinia Amino Alcohol Kinase and the Amino Alcohol O-Phosphate Lyase

ATP-dependent amino alcohol kinase and amino alcohol O-phosphate lyase activities have been detected in several Pseudomonas and Erwinia species, including Pseudomonas sp. P6 (NCIB10431), Pseudomonas putida NCIB 10558 (Jones et al. (1973) Biochem. J. 134:167-182), Erwinia carotovora, Erwinia amanas, Erwina milletiae, and Erwinia atroseptica (Jones et al. (1973) Biochem. J. 134:959-968). In these studies, the extracts of the above species were shown to have activity for the enzymatic conversion of aminopropanol through aminopropanol O-phosphate to propionaldehyde, and the conversion of ethanolamine through ethanolamine O-phosphate to acetaldehyde.

The genomic sequence of the Erwinia atroseptica strain in which these activities were reported to exist (now designated as Erwinia carotovora subsp. atroseptica strain SCR11043 (ATCC BAA-672)) has been determined at the Sanger Institute (Bell et al. Proc. Natl. Acad. Sci. USA 101(30): 11105-11110). Analysis of the putative kinases in the Erwinia carotovora subsp. atroseptica genome revealed an operon sequence (SEQ ID NO: 275) encoding a putative protein (ECA2059; SEQ ID NO:124) that is 39% identical to a Rhizobium loti homoserine kinase and a putative class-III pyridoxal phosphate (PLP)-dependent aminotransferase (ECA2060; SEQ ID NO:126) that is 58% identical to a putative aminotransferase from Rhizobium meliloti. It was expected that ECA2059 was an amino alcohol kinase and ECA2060 was an amino alcohol O-phosphate lyase which uses PLP as cofactor.

Cloning of the Putative Amino Alcohol Kinase and Putative Amino Alcohol O-Phosphase Lyase from Erwinia carotovora Subsp. atroseptica

Genomic DNA of Erwinia carotovora subsp. atroseptica (ATCC #: BAA-672D) was obtained from American Type Culture Collection (ATCC). The operon encoding the putative amino alcohol kinase (KA) and amino alcohol O-phosphate lyase (AT) was named KA-AT (SEQ ID NO: 275. This operon was amplified from the Erwinia genomic DNA by Phusion DNA polymerase (Finnzymes; via New England Biolabs; Ipswich, Mass.) using primers OT872 (SEQ. ID. No. 127) and OT873 (SEQ. ID. No128). A DNA fragment of 2.4 kb was obtained by the PCR reaction, which corresponds to the size of the KA-AT operon. The PCR product was digested with EcoRI and PstI restriction enzymes, and cloned into vector pKK223-3 (Amersham Biosciences; Piscataway, N.J.) which was digested with the same restriction enzymes. This produced plasmid pKK223.KA-AT, which contained the putative Erwinia amino alcohol kinase-lyase operon under control of the tac promoter. Similarly, plasmids pKK223.KA and pKK223.AT were made which placed the putative Erwinia kinase and the putative Erwinia lyase coding regions in separate vectors, each under the control of the tac promoter. For the PCR cloning of the KA coding region (SEQ ID NO:123), primers OT872 (SEQ. ID. No. 127) and OT879 (SEQ. ID. No. 129) were used; and for the PCR cloning of AT coding region (SEQ ID NO:125), primers OT873 (SEQ. ID. No. 128) and OT880 (SEQ. ID. No. 130) were used in the PCR amplifications, which generated PCR products of 1.1 kb and 1.3 kb respectively. The PCR products were each digested with EcoRI and PstI, and ligated into vector pKK223-3 to generate pKK223.KA and pKK223.AT.

In Vivo Activity of the Putative Amino Alcohol Kinase and Putative Amino Alcohol O-Phosphase Lyase from Erwinia carotovora Subsp. atroseptica

Plasmids pKK223.KA-AT, pKK223.KA, pKK223.AT and pKK223-3 were transformed into the E. coli MG1655 strain. The transformants were restreaked onto a MOPS minimal media plate containing 1% glucose, 0.5% aminopropanol as a sole nitrogen source, 1 mM IPTG and 100 μg/mL ampicillin. Expression of KA-AT, KA and AT genes were induced by the IPTG. A control plate had no IPTG included. The plates were incubated at 37° C. for 7 days. On the plate with IPTG, only the strain MG1655/pKK223.KA-AT grew, while all the other three strains did not grow. On the plate without added IPTG, the strain MG1655/pKK223.KA-AT grew, but the colonies were significantly smaller than those on the IPTG-containing plate, which corresponds to the lower expression levels of KA and AT in the uninduced cells. None of the other three strains grew on this plate. This indicates that the co-expression of the putative Erwinia KA and AT genes provided sufficient enzyme activities that allowed the E. coli strain MG1655/pKK223.KA-AT to utilize aminopropanol as a sole nitrogen source. Expression of each individual enzyme of either KA or AT was not sufficient to provide such enzyme activity in vivo.

Example 15 In Vitro Activity of Erwinia Putative Amino Alcohol Kinase and Amino Alcohol O-Phosphate Lyase

Subcloning of the Erwinia KA-AT Operon into the pBAD.HisB Vector and Induction of Protein Expression

The protein expression levels of Erwinia putative KA and AT enzymes expressed in MG1655 cells from the pKK223.KA-AT vector were analyzed by SDS-PAGE analysis. The expression level of the Erwinia AT enzyme was relatively low, with a new protein band detected at the correct molecular weight of 46 kD in the soluble fraction of a cell extract, while no new protein band was detected at the size predicted for the KA enzyme.

In an effort to improve the expression of the Erwinia putative KA and AT genes, the KA-AT operon was subcloned into the EcoRI and HindIII sites of vector pBAD.HisB-EcoRI. pBAD.HisB-EcoRI was derived from the pBAD.HisB vector (Invitrogen), by replacing the NcoI site in pBAD.HisB with an EcoRI site via QuickChange site-directed mutagenesis (Stratagene, La Jolla, Calif.) using primers OT909 (SEQ ID.# 131) & OT910 (SEQ ID.# 132). In the constructed plasmid pBAD.KA-AT, the KA-AT operon was placed directly under control of the araB promoter (without His-tag).

The pBAD.KA-AT plasmid was transformed into the E. coli TOP10 strain. A 50 mL culture of TOP10/pBAD.KA-AT strain was grown to mid log phase (OD₆₀₀=0.6) in LB, 100 μg/mL ampicillin media at 37° C. with shaking at 250 rpm. The culture was induced by addition of L-arabinose to a final concentration of 0.1% (w/v), and it was further incubated at 37° C. for 5 h before harvesting by centrifugation. The cell pellet was resuspended in ice cold 50 mM Tris-HCl, pH 8.0, and disrupted by sonication on ice with a Fischer Sonic Model 300 Dismembrator (Fischer, Pittsburgh, Pa.) at 50% power, repeating four cycles of 30 seconds sonication with 60 seconds rest in-between each cycle. Each sonicated sample was centrifuged (15,000×g, 4 min, 4° C.). Clarified cell free extracts were analyzed for protein expression level and amino alcohol O-phosphate lyase activity.

Chemical Synthesis of Aminobutanol O-Phosphate and Aminopropanol O-Phosphate

The substrate (R,R)-3-amino-2-butanol O-phosphate was synthesized by a method based on that reported by Ferrari and Ferrari (U.S. Pat. No. 2,730,542 [1956]) for phosphoethanolamine: 10 mmol of H₃PO₄ in a 50% (w/v) aqueous solution was mixed with a 50% (w/v) solution of (R,R)-3-amino-2-butanol (Bridge Organics; Vicksburg, Mich.) while stirring on ice. After mixing, the solution was slowly warmed to room temperature and then stirred under vacuum and heated to 70° C. After 1 h at 70° C., the temperature was slowly increased to 185° C. and maintained there for an additional 2 h. At that time, the reaction was cooled to room temperature and the vacuum released. The remaining material was dissolved in water, and analysis by NMR indicated that 80% of the starting material was converted to product with 20% remaining unreacted. No additional products were observed.

The additional substrates (2R,3S)-3-amino-2-butanol O-phosphate and (2S,3R)-3-amino-2-butanol O-phosphate were synthesized by the same procedure using a 1:1 mixture of (2R,3S)-3-amino-2-butanol and (2S,3R)-3-amino-2-butanol (synthesized as described in Example 13) as the starting material. DL-1-amino-2-propanol O-phosphate, (S)-2-amino-1-propanol O-phosphate, and (R)-2-amino-1-propanol O-phosphate were synthesized by the same procedure using DL-1-amino-2-propanol, (R)-2-amino-1-propanol, or (S)-2-amino-1-propanol as the starting material.

Analysis of the Aminopropanol O-Phosphate Lyase Activity Encoded by the Putative Erwinia KA-AT Operon

The aminopropanol O-phosphate lyase assay was performed as described by Jones et al. (1973, Biochem. J. 134:167-182) and G. Gori et al. (1995, Chromatographia 40:336) The formation of propionaldehyde from aminopropanol O-phosphate was assayed calorimetrically with MBTH, which allows the detection of aldehyde formation. The reaction was performed as follows. In a 1 mL reaction, 100 μg cell free extract of E. coli TOP10/pBAD.KA-AT was added to 10 mM DL-1-amino-2-propanol O-phosphate in 100 mM Tris-HCl, pH 7.8, with 0.1 mM PLP. The reaction was incubated at 37° C. for 10 min and 30 min, with an aliquot of 100 μL reaction mixture removed at each time point and mixed with 100 μL of 6 mg/mL MBTH in 375 mM glycine-HCl, pH 2.7. This mixture was incubated at 100° C. for 3 min, cooled on ice for 15-30 s, and 1 mL of 3.3 mg/mL FeCl₃.6H₂O (in 10 mM HCl) was added, followed by incubation for 30 min at room temperature. The absorbance of the reaction mixture which contains the aldehyde-MBTH adduct, was measured at 670 nm. The results of the assay are listed in Table 9. In the presence of the aminopropanol phosphate substrate, PLP and cell free extract, formation of aldehyde was detected, as indicated by an Abs₆₇₀ that was higher than the control background of up to 0.3. In the absence of either the substrate or the cell free extract, no aldehyde formation was detected. In the absence of added PLP, somewhat less amount aldehyde was detected, presumably due to the presence of PLP in the cell free extract. Cell free extract of the uninduced TOP10/pBAD.KA-AT-culture did not produce any detectable aldehyde in the reaction. These results indicated that the putative Erwinia amino alcohol O-phosphate lyase does catalyze the conversion of aminopropanol O-phosphate to propionaldehyde.

TABLE 9 Aminopropanol O-phosphate lyase assay. Sample 1 was the cell free extract of a non-induced control of E. coli TOP10/pBAD.KA-AT. Samples 2-5 contained the cell free extract of the induced culture E. coli TOP10/pBAD.KA-AT. Enzyme Induction Aminopropanol extract Sample by 0.1% O- (100 OD₆₇₀, OD₆₇₀, Number arabinose phosphate PLP μg/mL) 10 min 30 min 1 uninduced (+) (+) (+) 0.262 0.255 2 induced (+) (+) (+) 1.229 2.264 3 induced (−) (+) (+) 0.303 0.223 4 induced (+) (−) (+) 0.855 1.454 5 induced (+) (+) (−) 0.156 0.065

Analysis of the Activity of the Erwinia Amino Alcohol O-Phosphate Lyase Towards Aminobutanol O-Phosphate Substrate

The activity of the amino alcohol O-phosphate lyase towards the aminobutanol O-phosphate substrates was studied under the same conditions as described above. The reaction was carried out at 37° C. overnight in a 1 mL reaction that contained 100 μg of cell free extract of E. coli TOP10/pBAD.KA-AT, 10 mM aminobutanol O-phosphate (either the mixture of (R,R)+(S,S) or the mixture of (R,S)+(S,R) isomers described in Example 15) in 100 mM Tris-HCl, pH 7.8, with 0.1 mM PLP. An aliquot of 100 μL reaction mixture was removed and the 2-butanone product was detected using the MBTH derivatization method described in the General Methods. The two peaks representing the derivatized 2-butanone isomers were observed. Therefore the Erwinia amino alcohol O-phosphate lyase is an aminobutanol phosphate phospho-lyase in addition to an aminopropanol phosphate phospho-lyase.

Analysis of the Activity of the Erwinia Amino Alcohol O-Phosphate Lyase Towards Stereoisomers of Aminopropanol O-Phosphate and Aminobutanol O-Phosphate

The activity of the Erwinia amino alcohol O-phosphate lyase towards various stereoisomers of aminopropanol O-phosphate and aminobutanol O-phosphate was studied under the same conditions as described above. In the presence of the Erwinia amino alcohol O-phosphate lyase, both (R) and (S)-2-amino-1-propanol O-phosphate were converted to propanone by the enzyme, but the product yield was much higher with the (S) isomer. The enzyme also produced butanone from both mixtures of 3-amino)-2-butanol O-phosphate isomers, with a higher product yield found in the reaction containing the (R,S) and (S,R) substrate isomers. Both propanone and butanone products were derivatized by MBTH, and detected by HPLC as described in General Methods.

Optimization of the Gene Expression Level for the Erwinia Amino Alcohol Kinase and Amino Alcohol O-Phosphate Lyase

In order to improve the expression levels for the Erwinia amino alcohol kinase and the amino alcohol O-phosphate lyase in E. coli, codon optimized coding regions for both enzymes (named EKA: SEQ ID NO:155 and EAT: SEQ ID NO:156 respectively) were synthesized by DNA2.0 (Redwood City, Calif.). Each coding region was synthesized with 5′ and 3′ tails including restriction sites for cloning: EKA has 5′ BbsI and 3′ EcoRI, HindIII sites; EAT has 5′ EcoRI and 3′ HindIII sites. The EKA and EAT coding regions were provided from DNA2.0 as plasmids pEKA and pEAT, which were in the pJ51 vector of DNA2.0. The EKA optimized coding region was subcloned by ligating a BbsI and HindIII digested fragment of pEKA into the pBAD.HisB vector between the NcoII and HindIII sites, to generate plasmid PBAD.EKA. In the resulting plasmid the coding region is 5′ to the His tag, so a coding region for an N-terminus His₆ tag fused to the Erwinia amino alcohol kinase was constructed by performing a QuickChange site-directed mutagenesis reaction using primers SEQ ID NO:157 and SEQ ID NO:158 to generate vector pBAD.His-EKA.

pBAD.His-EKA was transformed into E. coli strain BL21AI (F⁻ ompT hsdSB (rB⁻mB⁻) gal dcm araB::T7RNAP-tetA; Invitrogen) to produce strain BL21AI/pBAD.HisA-EKA. A 50 mL culture of BL21AI/pBAD.HisA-EKA was grown to mid-log stage (OD₆₀₀=0.6), induced with 0.1% arabinose, and further incubated at 30° C. overnight. Cell free extracts were prepared by sonication. The His₆-tagged fusion protein of Erwinia amino alcohol kinase was purified using the ProBond™ Purification System (Invitrogen) under non-denaturing purification conditions following the manufacture's instructions.

Prophetic Result

The kinase activity of the His₆-tagged Erwinia amino alcohol kinase is analyzed by the ADP Quest Assay (DiscoveRx, Fremont, Calif.) following the manufacture's instructions. This is a biochemical assay that measures the accumulation of ADP, a product of the amino alcohol kinase reaction using either aminopropanol or aminobutanol as substrate. 10 mM substrate is mixed with His₆-tagged Erwinia amino alcohol kinase, in 100 mM Tris-HCl, pH 7.8, 10 mM MgCl₂, 2 mM KCl, 0.1 mM ATP, and incubated at 37° C. for 1 h in a 0.2 mL reaction. ADP reagent A (100 μL) and ADP reagent B (200 μL) are added and the mixture is incubated at room temperature for 30 min. The fluorescence signal indicating activity is measured with excitation wavelength of 530 nm and emission wavelength of 590 nm.

Example 16 Expression of Entire Pathway 3 Construction of Vector pCLBudAB-ter-T5chnA

The vector pTrc99a::BudABC (described in Example 9) is digested with EcoRI, and the DNA is treated with Klenow DNA polymerase to blunt the ends. The blunted vector is subsequently digested with SpeI to yield a 2.5 kb fragment containing the budA and budB genes. The vector pCL1925-ter-T5chnA (described in Example 9) is digested with HindIII, and the DNA was treated with Klenow DNA polymerase to blunt the ends. The blunted vector is subsequently digested with XbaI to yield a 4.6 kb fragment which is then ligated to the budAB fragment from pTrc99a::BudABC. The resulting plasmid, designated pCLBudAB-ter-T5chnA, is used to transform E. coli Top10 cells, and single colonies are screened for proper plasmid structure by PCR using primers pCL1925vecF (SEQ ID NO:62) and N84seqR3 (SEQ ID NO:159). Plasmid is prepared from a single colony which yields a PCR product of the expected size of 1.4 kb.

Construction of Vector pKK223.KA-AT-APT

The APT gene is amplified from the vector pBAD.APT (described in Example 12) by PCR using primers APTfor (SEQ ID NO:162; 5′ includes RBS and SmaI site) and APTrev (SEQ ID NO:163; 3′ adds SmaI site). The product of expected size of 1.7 kbp is gel purified and digested with SmaI to yield blunt ends. The vector pKK223.KA-AT (described in Example 14) is digested with PstI, and the DNA is treated with Klenow DNA polymerase to blunt the ends. The resulting DNA fragment is ligated with the SmaI-digested PCR product, and the ligation product is used to transform E. Coli Top10 cells. Individual ampicillin resistant colonies are screened by PCR using primers OT872 (SEQ ID NO:127) and APTrev (SEQ ID NO:163). The presence of a PCR product of the expected size of 4.1 kbp indicates that the gene encoding APT is present and oriented in the same direction as the genes encoding KA and AT. The sequence of the insert is verified using the primers APTseqRev (SEQ ID NO:160) and APTseqFor (SEQ ID NO:161). This plasmid is named pKK223.KA-AT-APT. Proper expression of all three genes is verified by growing a 5 mL culture of Top10/pKK223.KA-AT-APT in LB+100 μg/mL ampicillin at 37° C. with shaking. When the OD₆₀₀ reaches ˜0.8, expression of the genes on the plasmid is induced by addition of IPTG to 0.4 mM. The expression is evaluated by SDS PAGE and activity assays as described above.

Construction of 2-Butanol Production Strain and Production of 2-Butanone and 2-Butanol

E. coli strain MG1655 is transformed with both pKK223.KA-AT-APT and pCLBudAB-ter-T5chnA, and transformants selected for ampicillin and spectinomycin resistance, indicative of the presence of the plasmids. The cells are inoculated into shake flasks (approximately 175 mL total volume) containing 50 or 150 mL of TM3a/glucose medium (with appropriate antibiotics) to represent medium and low oxygen conditions, respectively. IPTG is added to 0.4 mM to induce expression of genes from pKK223.KA-AT-APT. As a negative control, MG1655 cells are grown in the same medium lacking antibiotics. The flasks are inoculated at a starting OD₆₀₀ of <0.01 and incubated at 34° C. with shaking at 300 rpm for 24 h. The flasks containing 50 mL of medium are capped with vented caps; the flasks containing 150 mL are capped with non-vented caps to minimize air exchange. The MG1655/pKK223.KA-AT-APT/pCLBudAB-ter-T5chnA strain comprising a 2-butanol biosynthetic pathway produces both 2-butanone and 2-butanol under low and medium oxygen conditions while the negative control strain does not produce detectable levels of either 2-butanone or 2-butanol.

Example 17 Characterization of Glycerol Dehydratase Butanediol Dehydratase Activity

Glycerol dehydratase (E.C. 4.2.1.30) and diol dehydratase (E.C. 4.2.1.28), while structurally related, are often distinguished in the art based on various differences that include substrate specificity. This example demonstrates that glycerol dehydratase converts meso-2,3-butanediol to 2-butanone. The recombinant E. coli strain KLP23/pSYCO12, comprising Klebsiella pneumoniae genes encoding the multiple subunits of glycerol dehydratase (alpha: SEQ ID NO:145 (coding region) and 146 (protein); beta: SEQ ID NO: 147 (coding region) and 148 (protein); and gamma: SEQ ID NO: 149 (coding region) and 150 (protein)) and Klebsiella pneumoniae genes encoding the multiple subunits of glycerol dehydratase reactivase (large subunit, SEQ ID NO: 151 (coding region) and 152 (protein); and small subunit, SEQ ID NO: 153 (coding region) and 154 (protein)), is described in Emptage et al. U.S. Pat. No. 6,514,733 and in WO 2003089621, which are herein incorporated by reference. A crude, cell free extract of KLP23/pSYCO12 was prepared by methods known to one skilled in the art. Enzyme assay was performed in the absence of light in 80 mM HEPES buffer, pH 8.2 at 37° C. with 12 μM coenzyme B₁₂ and 10 mM meso-2,3-butanediol. The formation of 2-butanone was monitored by HPLC (Shodex SH-1011 column and SH-G guard column with refractive index detection; 0.01 M H₂SO₄ as the mobile phase at a flow rate of 0.5 mL/min and a column temperature of 50° C.; 2-butanone retention time=40.2 min). The rate of 2-butanone formation by the glycerol dehydratase preparation was determined to be 0.4 nmol/min/mg of crude protein.

Example 18 Structural Analysis of Diol/Glycerol Dehydratases through Generation and Validation of a Profile HMM for Experimentally Proven Diol/Glycerol Dehydratases

The diol dehydratase and glycerol dehydratase enzymes belong to the enzyme classes 4.2.1.28 and 4.2.1.30, respectively. The enzymes in both classes are each a complex of three subunits: large (also called alpha), medium (also called beta) and small (also called gamma). In some glycerol dehydratases the large and medium subunits were found to be fused.

Identifying Family Members by Sequence

The Klebsiella oxytoca butanediol dehydratase enzyme was used as a prototype enzyme for identifying a family of diol and glycerol dehydratase enzymes. The amino acid sequences of the alpha (GenBank No: BAA08099; SEQ ID NO: 8), beta (GenBank No: BAA08100; SEQ ID NO: 10) and gamma (GenBank No: BAA08101; SEQ ID NO: 12) subunits were each run as the query sequence in a BLASTP search against the GenBank non-redundant protein database using default parameters. Sequences with relevant matches were extracted. Relevance was judged by the E-value score, protein definition, details included in the GenBank report for the matched proteins, and literature review of the topic. For the large subunit, the BLAST output showed an abrupt decrease in the E-value from e-20 to an E-value of 1.5. All sequence matches with an E-value of 1.5 or larger had definitions inconsistent with them being dehydratases. Many of these sequences were labeled as DNA-directed RNA polymerase beta subunits. There were matches with E-values around e-20, which were partial sequences. Sequences with no annotation were included if the E-value was less than 1.5.

Using the Klebsiella oxytoca butanediol dehydratase alpha subunit as a query, 50 homologs were identified as members of this family of proteins. This group included some sequences that were not full length proteins. The full length sequences identified for the alpha subunit family of diol/glycerol dehydratases are the prototype SEQ ID No: 8 and SEQ ID NOs: 93, 99, 105, 135, 138, 141, 146, 164, 167, 170, 173, 176, 179, 182, 185, 188, 191, 194, 197, 200, 203, 206, 209, 212, 215, 218, 221, 224, 227, 130, 243, 254, 255, 256, 257, 258, and 259. SEQ ID NOs: 233, 235, 237, 239, 241, 246, 247 include both the alpha and beta subunits, which are fused in these cases.

Using the Klebsiella oxytoca butanediol dehydratase beta subunit as a query, 51 homologs were identified as members of this family of proteins. This group included some sequences that were not full length proteins. The full length sequences identified for the beta subunit family of diol/glycerol dehydratases are the prototype SEQ ID No: 10 and SEQ ID NOs: 95, 101, 107, 136, 139, 142, 148, 165, 168, 171, 174, 177, 180, 183, 186, 189, 192, 195, 198, 201, 204, 207, 210, 213, 216, 219, 222, 225, 228, 231, 244, 250, 252, 260, 261, 262, 263, 364, 265, 266, and 167.

Using the Klebsiella oxytoca butanediol dehydratase gamma subunit as a query, 48 homologs were identified as members of this family of proteins. This group included some sequences that were not full length proteins. The full length sequences identified for the gamma subunit family of diol/glycerol dehydratases are the prototype SEQ ID No: 12 and SEQ ID NOs: 97, 103, 109, 137, 140, 143, 150, 166, 169, 172, 175, 178, 181, 184, 187, 190, 193, 196, 199, 202, 205, 208, 211, 214, 217, 220, 223, 226, 229, 232, 234, 236, 238, 240, 242, 245, 248, 249, 251, 253, 268, 270, 271, 272, 273, and 274.

Identifying Family Members with Experimentally Assessed Function

For each sequence identified through the analysis described above, a search for experimental evidence of its biochemical function was conducted in the BRENDA, UniProt and NCBI Entrez databases. BRENDA is a human-curated database that contains detailed information about enzyme kinetic, physical, and biochemical properties extracted from the experimental literature and with links to the relevant databases (Cologne University Bioinformatics Center). The UniProt Knowledgebase is composed of a human-curated part the Swiss-Prot database and of a machine annotated supplement the TrEMBL database. The curated Swiss-Prot database (Swiss Institute of Bioinformatics) provides a high level of protein annotation that includes domain architecture, post-translational modifications and sequence variants. NCBI Entrez is the integrated, text-based search and retrieval system used at NCBI (National Center for Biotechnology Information, Bethesda, Md.) for the major databases, including PubMed, Nucleotide and Protein Sequences, Protein Structures, Complete Genomes, and Taxonomy.

Through the analysis of information and references identified from these databases, eight diol/glycerol dehydratases with experimentally verified function as a diol or glycerol dehydratase were identified. These are given in Table 10.

TABLE 10 Diol/glycerol dehydratases with experimentally verified function SEQ organism subunit Genbank # ID No type reference Klebsiella large gi|6980836 8 diol Shibata et al., oxytoca Structure 1999; K. oxytoca medium gi|6980837 10 diol 7: 997-1008 K. oxytoca small gi|6980838 12 diol Klebsialla large gi|4063702 105 diol Tobimatsu et al., pneumoniae 1998; Biosci. K. pneumoniae medium gi|94470233 107 diol Biotechnol. Biochem. K. pneumoniae small gi|4063704 109 diol 62: 1774-1777 Clostridium large gi|3360389 135 glycerol Macis et al., FEMS pasteurianum Microbiol Lett. 1998; C. pasteurianum medium gi|3360390 136 glycerol 164(1): 21-8 C. pasteurianum small gi|3360391 137 glycerol Escherichia large gi|60099613 138 glycerol Sönke et al. blattae J. of Mol. Micro. and E. blattae medium gi|57340191 139 glycerol Biotech. 2004; 8: 150-168 E. blattae small gi|57340192 140 glycerol Klebsialla large gi|24158719 146 glycerol Willard, Thesis pneumoniae (1994), U of K. pneumoniae medium gi|24158720 148 glycerol Wisconsin-Madison K. pneumoniae small gi|24158721 150 glycerol Citrobacter large gi|1169287 141 glycerol Seyfried, Gottschalk; freundii J. Bacteriol. C. freundii medium gi|1229154 142 glycerol 178: 5793-5796 C. freundii small gi|1229155 143 glycerol (1996) Lactobacillus large gi|116334196 164 diol Schuetz and Radler brevis 1984; Arch. Microbiol. L. brevis medium gi|116334195 165 diol 139, 366-370 L. brevis small gi|116334194 166 diol Lactobacillus large gi|18857678 99 diol Sauvageot et al., collinoides 2002; Eur J Biochem. L. collinoides medium gi|18857679 101 diol 269(22): 5731-7. L. collinoides small gi|18857680 103 diol

The set of 8 amino acid sequences of each subunit from diol/glycerol dehydratases with experimentally determined function, listed in Table 10, were compared by making a multiple sequence alignment using ClustalW with default parameters. The % identity for the large subunit sequences ranged from 97.6% to 58.4%. The % identity for the medium subunit sequences ranged from 89.5% to 41.7%. The % identity for the small subunit sequences ranged from 83.3% to 36.4%. Thus the amount of sequence identity between some subunit sequences was very low (such as 36.4%, 41.7%) even though these subunits were known to be components of enzymes known through experimental data to perform the same function. The low level of % sequence identity made it impractical to use this criterion for structure/function correlations

Sequence Relationship of Experimentally Verified Diol/Glyderol Dehydratases to other Diol/Glycerol Dehydratases

To perform this analysis, highly redundant sequences that are >95% identical were removed from the sequence set for large, medium or small subunits, except that all experimentally verified function sequences were retained. Truncated or partial protein sequences were also removed. A multiple sequence alignment was performed on the remaining sequences using ClustalW with default parameters. The % identity for the large subunits ranged from 97.6% (highest % is from multiple experimentally verified sequences) to 42.8%. The % identity for the medium subunits ranged from 91.9% to 26.4%. The % identity for the small subunits ranged from 85.2% to 20.5%. These % identities are similar ranges to the % identities for the experimentally verified sequences.

Based on the multiple sequence alignments, phylogenetic trees were built using the neighbor-joining algorithm (as implemented in the MEGA software version 3.1 package; Kumar et al., 2004 Briefings in Bioinformatics 5:150-163.) The phylogenetic trees are shown in FIGS. 2 (large subunit), 3 (medium subunit), and 4 (small subunit), with the identities of the mapped sequences listed in a key for each figure. As seen from the positions marked for the experimentally verified function sequences (in dark and light grey circles for diol dehydratase and glycerol dehydratase, respectively), these sequences are spread over most of the tree. However, each tree does include a branch with no experimentally verified members, but which appears to belong to the diol/glycerol dehydratase family.

Building a Profile Hidden Markov Model (HMM) of the Diol/Glycerol-Dehydratase Family Based on the Sets of Eight Subunit Sequences

An alternative structure/function characterization of the sets of subunits of the diol/glycerol dehydratase family of enzymes was performed using the HMMER software package (The theory behind profile HMMs is described in R. Durbin, S. Eddy, A. Krogh, and G. Mitchison, Biological sequence analysis: probabilistic models of proteins and nucleic acids, Cambridge University Press, 1998; Krogh et al., 1994; J. Mol. Biol. 235:1501-1531), following the user guide which is available from HMMER (Janelia Farm Research Campus, Ashburn, Va.).

Each set of 8 amino acid sequences for the large, medium, and small subunits of functionally characterized diol/glycerol dehydratases (in Table 10) was separately analyzed using the HMMER software program. The output of the HMMER software program is a Profile Hidden Markov Model (HMM) that characterizes the input sequences. As stated in the user guide, Profile HMMs are statistical models of multiple sequence alignments. They capture position-specific information about how conserved each column of the alignment is, and which amino acid residues are most likely to occur at each position. Thus HMMs have a formal probabilistic basis. Profile HMMs for a large number of protein families are publicly available in the PFAM database (Janelia Farm Research Campus, Ashburn, Va.).

Each Profile HMM was built as follows:

Step 1. Build a Sequence Alignment

The eight sequences for the large subunit of the functionally verified diol/glycerol dehydratases (SEQ ID NOs:8, 99, 105, 135, 138, 141, 146, and 164) were aligned using Clustal W with default parameters. The same was done for the set of medium subunit sequences (SEQ ID NOs:10, 101, 107, 136, 139, 142, 148, and 165) and the set of small subunit sequences (SEQ ID NOs:12, 103, 109, 137, 140, 143, 150 and 166).

Step 2. Build a Profile HMM

The hmmbuild program was run on each set of the aligned sequences using default parameters. hmmbuild reads the multiple sequence alignment file, builds a new Profile HMM, and saves the Profile HMM to file. Using this program an un-calibrated profile was generated from the multiple alignment for each set of subunit sequences described above.

The following information based on the HMMER software user guide gives some description of the way that the hmmbuild program prepares a Profile HMM. A Profile HMM is capable of modeling gapped alignments, e.g. including insertions and deletions, which lets the software describe a complete conserved domain (rather than just a small ungapped motif). Insertions and deletions are modeled using insertion (I) states and deletion (D) states. All columns that contain more than a certain fraction x of gap characters will be assigned as an insert column. By default, x is set to 0.5. Each match state has an I and a D state associated with it. HMMER calls a group of three states (M/D/I) at the same consensus position in the alignment a “node”. These states are interconnected with arrows called state transition probabilities. M and I states are emitters, while D states are silent. The transitions are arranged so that at each node, either the M state is used (and a residue is aligned and scored) or the D state is used (and no residue is aligned, resulting in a deletion-gap character, ‘-’). Insertions occur between nodes, and I states have a self-transition, allowing one or more inserted residues to occur between consensus columns.

The scores of residues in a match state (i.e. match state emission scores), or in an insert state (i.e. insert state emission scores) are proportional to Log_(—)2 (p_x)/(null_x). Where p_x is the probability of an amino acid residue, at a particular position in the alignment, according to the Profile HMM and null_x is the probability according to the Null model. The Null model is a simple one state probabilistic model with pre-calculated set of emission probabilities for each of the 20 amino acids derived from the distribution of amino acids in the SWISSPROT release 24.

State transition scores are also calculated as log odds parameters and are proportional to Log_(—)2 (t_x). Where t_x is the probability of transiting to an emitter or non-emitter state.

Step 3. Calibrate the Profile HMM

Each Profile HMM was read using hmmcalibrate which scores a large number of synthesized random sequences with the Profile (the default number of synthetic sequences used is 5,000), fits an extreme value distribution (EVD) to the histogram of those scores, and re-saves the HMM file now including the EVD parameters. These EVD parameters (μ and λ) are used to calculate the E-values of bit scores when the profile is searched against a protein sequence database. hmmcalibrate writes two parameters into the HMM file on a line labeled “EVD”: these parameters are the μ (location) and λ (scale) parameters of an extreme value distribution (EVD) that best fits a histogram of scores calculated on randomly generated sequences of about the same length and residue composition as SWISS-PROT. This calibration was done once for each Profile HMM.

The calibrated Profiles HMM for the large subunit, medium subunit, and small subunit sets of sequences are provided in the appendix as alpha Profile HMM, beta Profile HMM, and gamma Profile HMM Excel charts. Each Profile HMM is provided in a chart that gives the probability of each amino acid occurring at each position in the amino acid sequence. The highest probability is highlighted for each position. Table 11 shows a few lines of the Profile HMM prepared for the large subunits of diol/glycerol dehydratases with function experimentally verified.

TABLE 11 A portion of the large subunit Profile HMM. HMM A C D E F G H I K L m -> m m-> i m-> d i-> m i-> i d-> m d-> d b-> m m-> e −585 * −1585 1(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 — −149 −500 233 43 −381 399 106 −626 210 −466 — −33 −6045 −7087 −894 −1115 −701 −1378 −585 * 2(K) −603 −1732 −469 811 −2182 −1397 205 −1770 1954 −1654 — −149 −500 233 43 −381 399 106 −626 210 −466 — −350 −6045 −2321 −894 −1115 −701 −1378 * * 3(R) −781 −1268 −994 −512 −1638 −1313 −151 −1479 778 −1459 — −149 −500 233 43 −381 399 106 −626 210 −466 — −41 −5736 −6778 −894 −1115 −535 −1690 * * HMM M N P Q R S T V W Y 1(M) 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 — −720 275 394 45 96 359 117 −369 −294 −249 — 2(K) −860 −59 −1465 629 1868 −495 −471 −1439 −1719 −1294 — −720 275 394 45 96 359 117 −369 −294 −246 — 3(R) −913 −539 −1557 139 3077 −831 −760 −1263 −1437 −1152 — −720 275 394 45 96 359 117 −369 −294 −249 — The amino acids are represented by the one letter code.

The first line for each position reports the match emission scores: probability for each amino acid to be in that state (highest score is highlighted). The second line reports the insert emission scores, and the third line reports on state transition scores: M→M, M→I, M→D; I→M, I→I; D→M, D→D; B→M; M→E.

Table 11 shows that for the large subunits, methionine has a 4141 probability of being in the first position, the highest probability which is highlighted. In the second position lysine has the highest probability, which is 1954. In the third position arginine has the highest probability, which is 3077.

Step 4. Test the Specificity and Sensitivity of the Built Profile HMMs

Each Profile HMM was evaluated using hmmsearch, which reads a Profile HMM from hmmfile and searches a sequence file for significantly similar sequence matches. The sequence file searched was the GenBank non-redundant protein database. The size of the database (Z parameter) was set to 1 billion. This size setting ensures that significant E-values against the current database will remain significant in the foreseeable future. The E-value cutoff was set at 10.

The Profile HMMs for the large, medium and small subunits of diol/glycerol dehydratases with experimentally verified function were specific in that only diol/glycerol dehydratase subunits were recovered, as indicated by the annotation of the matched sequences, and sensitive in that even partial sequences of diol/glycerol dehydratase subunits were recovered. Each of the recovered sequences had an E-value of 0.01 or less.

All of the sequences on the phylogenetic trees in FIGS. 2, 3, and 4 were recovered in the Profile HMM matching. All sequences in the branches of the trees which contain no sequence with experimentally verified function were matched. Thus all of the diol and glycerol dehydratases were linked to the 8 diol and glycerol dehydratases with experimentally verified function through matching with the Profile HMMs for the large, medium, or small subunits of the enzymes. The full length diol and glycerol dehydratase subunits that match the Profile HMMs have the following SEQ ID NOs:

Large (alpha) subunits: 8, 93, 99, 105, 135, 138, 141, 146, 164, 167, 170, 173, 176, 179, 182, 185, 188, 191, 194, 197, 200, 203, 206, 209, 212, 215, 218, 221, 224, 227, 130, 243, 254, 255, 256, 257, 258, and 259. Large+medium subunits fused (large subunit and medium subunit portion matches the large Profile and medium Profile, respectively): 233, 235, 237, 239, 241, 246, and 247. Medium (beta) subunits: 10, 95, 101, 107, 136, 139, 142, 148, 165, 168, 171, 174, 177, 180, 183, 186, 189, 192, 195, 198, 201, 204, 207, 210, 213, 216, 219, 222, 225, 228, 231, 244, 250, 252, 260, 261, 262, 263, 364, 265, 266, and 167. Small (gamma) subunits: 12, 97, 103, 109, 137, 140, 143, 150, 166, 169, 172, 175, 178, 181, 184, 187, 190, 193, 196, 199, 202, 205, 208, 211, 214, 217, 220, 223, 226, 229, 232, 234, 236, 238, 240, 242, 245, 248, 249, 251, 253, 268, 270, 271, 272, 273, and 274.

This analysis shows that the Profile HMM for each subunit, that was prepared using sequences with experimentally verified function, provides a structure that is linked to function of diol/glycerol dehydratase enzymes. Matching of all of the above sequences to the Profiles HMM in turn provides a structure/function link for these sequences.

TABLE 12 HMMER 2.0 [2.3.2] Program name and version NAME alpha_exp_seqs Name of the input sequence alignment file LENG 557 Length of the alignment: include indels ALPH Amino Type of residues MAP yes Map of the match states to the columns of the alignment COM hmmbuild alpha.hmm alpha_exp_seqs.aln Commands used to generate the file: this one means that hmmbuild (default patrameters) was applied to the alignment file COM hmmcalibrate alpha.hmm Commands used to generate the file: this one means that hmmcalibrate (default parametrs) was applied to the hmm profile NSEQ 8 Number of sequences in the alignment file DATE Fri Mar 30 19:02:15 2007 When was the file generated XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4 NULT −4 −8455 The transition probability distribution for the null model (single G state). NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644 EVD −264.989197 0.112643 The symbol emission probability distribution for the null model (G state); consists of K (e.g. 4 or 20) integers. The null probability used to convert these back to model probabilities is The extreme value distribution parameters μ and lambda respectively; both floating point values. Lambda is positive and nonzero. These values are set when the model is calibrated with hmmca HMM A C D E F G H I K L M N P Q R S T V W Y m->m m->i m->d i->m i->i d->m d->d b->m m->e −585 * −1585  1(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 1 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 −585 *  2(K) −603 −1732 −469 811 −2182 −1397 205 −1770 1954 −1654 −860 −59 −1465 629 1868 −495 −471 −1439 −1719 −1294 2 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −350 −6045 −2321 −894 −1115 −701 −1378 * *  3(R) −781 −1268 −994 −512 −1638 −1313 −151 −1479 778 −1459 −913 −539 −1557 136 3077 −831 −760 −1263 −1437 −1152 3 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5736 −6778 −894 −1115 −535 −1690 * *  4(Q) −149 −1077 25 226 −1650 −945 −85 −1408 309 −1505 −735 33 −1280 1816 −34 1644 −225 −1020 −1731 −1155 4 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  5(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 5 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  6(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 6 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  7(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 7 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  8(E) 37 −1709 562 2097 −2000 −1013 65 −1701 388 −1724 −924 314 −1290 1204 −72 −263 −376 −1331 −1960 −1293 8 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  9(V) 428 −898 −212 744 −1063 −1160 90 −556 881 −841 −94 22 −1284 414 20 −168 −65 941 −1268 −753 9 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  10(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 10 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  11(E) 995 −1189 274 1599 −1710 −927 −3 −1362 257 −1488 −682 191 −1227 366 −212 521 −177 −986 −1785 −1178 11 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  12(K) −509 −1745 1 892 −2124 −1242 174 −1769 2128 −1689 −880 106 −1385 1310 676 −383 −416 −1413 −1802 −1283 12 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  13(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 13 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  14(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 14 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  15(V) −886 −604 −2944 −2505 −743 −2731 −2045 1944 −2256 229 340 −2308 −2716 −2086 −2330 −1950 −884 2675 −1910 −1506 15 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  16(H) −457 −1514 304 388 −1218 −1098 2418 −1597 328 −1620 −886 2258 −1387 298 −36 −374 −457 −1264 −1448 −664 16 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  17(Q) −346 −1181 −403 128 −1300 −1307 121 −906 1166 365 −294 −69 −1397 1890 525 −356 −260 −706 −1373 −890 17 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  18(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 18 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  19(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 19 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  20(F) −1300 −1041 −2794 −2396 2872 −2678 −676 440 −2076 1465 808 −1982 −2569 −1598 −1976 −1862 −1239 98 −109 791 20 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  21(V) −922 −605 −3064 −2618 −752 −2841 −2139 2359 −2383 244 349 −2414 −2784 −2191 −2442 −2058 −911 2430 −1948 −1560 21 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  22(K) −395 −1326 −130 117 −1960 1239 −86 −1633 2073 −1681 −915 −74 −1403 278 289 −375 −423 −1258 −1820 −1352 22 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  23(E) −597 −1990 829 2406 −2276 −963 −75 −2069 89 −2078 −1318 1286 −1352 285 −438 −411 −615 −1663 −2255 −1512 23 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  24(W) −1926 −1577 −2416 −2370 192 −2070 −795 −1602 −1882 −1347 −1210 −2018 −2383 −1854 −1777 −2150 −1992 −1643 5696 560 24 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  25(P) −136 −963 748 291 −1174 −1080 −2 367 217 −956 −244 51 1547 318 −221 −177 −129 −438 −1415 −860 25 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  26(E) −704 −2274 1609 2494 −2517 −929 −118 −2327 −117 −2302 −1553 440 −1360 238 −777 −462 −729 −1891 −2491 −1671 26 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  27(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 27 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  28(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 28 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  29(F) −1300 −1032 −2939 −2479 2085 −2797 −1060 610 −2170 2072 1034 −2173 −2620 −1672 −2063 −1972 −1227 200 −496 232 29 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  30(I) −932 −616 −3069 −2623 −730 −2845 −2140 2586 −2385 279 372 −2420 −2786 −2188 −2440 −2065 −921 2194 −1936 −1554 30 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  31(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 31 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  32(M) −1097 −948 −2613 −2098 66 −2604 −1439 782 −1681 1487 3575 −2007 −2489 −1444 −1696 −1772 −1045 428 −1173 −886 32 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  33(E) −103 −1383 474 1130 −1629 −475 238 −1330 588 −1372 919 886 −1165 659 86 417 −51 −967 −1624 −981 33 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  34(S) 317 −370 −658 −616 −1967 1419 −807 −1713 −760 −1924 −1109 −411 −1154 −588 −1027 2116 −11 −1006 −2151 −1737 34 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  35(P) −405 −1216 −191 6 −1782 −1120 −188 −1451 1187 −1561 −850 −164 2497 138 133 −424 −456 −1124 −1757 −1301 35 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  36(N) −556 −847 −1097 −603 1232 −1683 201 −508 −426 −601 −46 2198 −1740 −261 −699 −699 −493 −395 1687 1610 36 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  37(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 37 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  38(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 38 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  39(K) 175 −1085 −247 253 −1457 −1107 109 −1024 1939 −1182 −399 40 −300 486 284 −143 −116 −221 −1500 −977 39 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  40(P) 213 −475 −556 −468 −1769 −628 −656 −1500 −525 −1718 −950 −359 2478 −426 −800 1259 −70 −926 −1971 −1515 40 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  41(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 41 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  42(I) −935 −625 −3052 −2609 −708 −2832 −2125 2677 −2364 307 391 −2408 −2777 −2168 −2419 −2052 −926 2060 −1920 −1540 42 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  43(K) −489 −1476 −535 74 −1899 −1347 163 −1474 2257 −1467 −693 −94 −1441 566 1161 −436 566 −1175 −1619 −1191 43 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  44(I) −935 −625 −3052 −2609 −708 −2832 −2125 2677 −2364 307 391 −2408 −2777 −2168 −2419 −2052 −926 2060 −1920 −1540 44 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  45(E) −190 −1482 610 1617 −1728 −1033 193 −1411 949 −1462 −616 305 −1203 607 49 −87 −141 −15 −1713 −1064 45 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  46(N) −508 −1893 708 1172 −2155 −1000 25 −1908 309 −1897 −1104 2413 −1320 976 −163 −338 −492 −1519 −2096 −1394 46 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  47(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 47 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  48(K) 398 −959 −358 223 −1120 −1205 148 −688 1297 −524 −121 6 −1289 492 847 −176 −70 241 −1260 −760 48 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  49(V) −905 −603 −3012 −2567 −752 −2794 −2098 2117 −2327 233 342 −2367 −2755 −2145 −2394 −2012 −898 2593 −1933 −1537 49 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  50(V) −367 −384 −1856 −1345 −565 −1819 −964 1486 −1115 20 389 −1247 −1954 −968 −1285 −941 1596 1684 −1282 −891 50 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  51(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 51 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  52(M) −1262 −1030 −2945 −2401 126 −2872 −1670 808 −2031 2152 2757 −2305 −2645 −1652 −1984 −2035 −1186 370 −1224 −1041 52 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  53(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 53 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  54(G) 291 −380 −629 −669 −2010 2409 −887 −1774 −878 −2000 −1199 −445 −1175 −694 −1118 1047 −53 −1050 −2199 −1795 54 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  55(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 55 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  56(K) 193 −1311 14 503 −1606 −1067 229 −1289 1052 −1334 −482 797 710 647 530 219 −57 −935 −1580 −977 56 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  57(R) 381 −1078 −400 189 −1317 −1223 150 −885 1141 −1041 −273 −15 −1318 514 1534 −224 −141 222 −1367 −879 57 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  58(A) 1262 −1516 1112 1074 −1844 −963 80 −1548 308 −1606 −781 326 −1230 469 −228 238 −247 −1170 −1872 −1200 58 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  59(D) −668 −2298 2567 1387 −2516 −916 −71 −2332 −49 −2279 −1509 475 −1334 882 −709 −420 −683 −1885 −2484 −1642 59 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  60(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 60 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  61(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 61 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  62(M) −1241 −1016 −2914 −2371 122 −2844 −1644 806 −1999 2066 2932 −2273 −2626 −1630 −1957 −2005 −1167 376 −1215 −1025 62 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  63(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 63 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  64(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 64 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  65(R) −439 −1400 −571 103 −1664 −1368 1527 −1291 1414 −314 −534 −77 −1418 594 1819 −398 −324 −1021 −1489 −1036 65 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  66(F) −1902 −1421 −2805 −2692 3214 −2763 351 −984 −2354 −657 −540 −1668 −2714 −1627 −2106 −1950 −1820 −1082 1026 3156 66 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  67(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 67 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  68(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 68 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  69(D) −196 −1649 1118 1061 −1949 −1018 231 −1691 1047 −1647 −758 919 −1183 668 786 −60 −144 −1263 −1826 −1142 69 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  70(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 70 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  71(G) 1432 −310 −885 −866 −1952 2267 −962 −1556 −974 −1871 −1097 −548 −1176 −789 −1180 127 −20 −901 −2164 −1815 71 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  72(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 72 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  73(N) −504 −1608 307 389 −1979 −1097 −14 −1734 1180 −1761 −1004 2578 −1393 350 215 −409 −498 −1377 −1890 −1303 73 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  74(L) −1081 −806 −3000 −2464 −132 −2843 −1790 1457 −2184 2042 966 −2321 −2664 −1813 −2161 −2002 −1025 1443 −1417 −1198 74 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  75(E) 88 −1806 1193 1543 −2082 −964 125 −1841 365 −1812 −954 1330 −322 537 −200 −160 −295 −1412 −2017 −1284 75 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  76(R) −369 −1625 140 1001 −1955 −1144 172 −1638 765 −1611 −784 1492 −1309 587 1643 −256 −302 −1274 −1773 −1195 76 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  77(A) 2326 −235 −1178 −992 −1593 −588 −902 −857 −875 −1381 −703 −620 −1200 −746 −1059 114 1127 −426 −1928 −1562 77 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  78(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 78 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  79(K) −149 −1571 689 1005 −1882 −1048 946 −1613 1386 −1568 −672 314 −1171 987 550 −26 −87 −1190 −1741 −1082 79 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  80(V) 995 −264 −1540 −1124 −837 −1157 −824 540 −925 −450 74 −897 −1545 −777 −1105 −359 819 1919 −1383 −997 80 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  81(M) −1089 −847 −2901 −2332 41 −2767 −1601 1436 −2029 1495 3271 −2205 −2566 −1630 −1990 −1898 −1014 653 −1227 −1042 81 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  82(K) 758 −1358 30 517 −1633 −495 241 −1317 1032 −1352 −498 858 −1194 944 553 −51 −69 −963 −1590 −984 82 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  83(M) −491 −492 −1884 −1331 −96 −1923 −814 701 −1026 1207 2815 −1254 −1949 −845 −1137 −1009 548 576 −949 −635 83 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  84(D) −824 −2383 2964 1142 −2628 −923 −210 −2461 −314 −2455 −1761 430 −1405 123 −1021 −562 −876 −2027 −2629 −1790 84 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  85(S) 1394 −260 −956 −822 −1809 −507 −852 −1408 −809 −1720 −938 −503 −1137 −658 −1033 2235 57 −788 −2055 −1662 85 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  86(V) −466 −415 −2075 −1571 −506 −1975 −1108 1171 −1302 688 460 −1436 −2079 −1152 −1427 −1111 573 2199 −1304 −923 86 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  87(K) −401 −1577 34 922 −1932 −1184 156 −1558 2117 −1563 −758 127 −1342 565 523 −304 237 −1220 −1742 −1203 87 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  88(I) −1120 −818 −3064 −2567 −204 −2914 −1888 2467 −2246 1556 866 −2411 −2743 −1931 −2224 −2108 −1072 1115 −1497 −1212 88 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  89(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 89 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  90(N) −477 −1612 −233 247 −2000 −1284 183 −1637 1365 −1583 −780 1891 −1397 598 1526 −382 −381 −1301 −1701 −1216 90 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  91(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 91 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  92(L) −1306 −1067 −2981 −2443 125 −2920 −1717 814 −2062 2337 2190 −2353 −2682 −1686 −2014 −2092 −1231 368 −1248 −1063 92 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  93(C) −206 3367 −2319 −1919 −689 −1494 −1270 951 −1603 −153 201 −1455 −1861 −1411 −1625 −746 −346 2000 −1422 −1027 93 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  94(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 94 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  95(I) −274 −522 −1274 −906 −613 −1430 −689 1794 −672 −125 232 −863 1767 −614 −872 −631 −339 493 −1235 −798 95 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  96(N) −479 −1529 346 390 −1262 −1084 1651 −1642 280 −1666 −940 2606 −1396 262 −86 −394 −490 −1304 −1493 −708 96 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  97(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 97 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  98(S) −161 −1453 838 607 −1778 −999 177 −1491 1000 −1517 −659 304 145 593 16 1073 −129 −1102 −1752 −1097 98 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * *  99(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 99 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 100(K) 639 −1359 130 937 −1666 −1054 189 −1344 1343 −1395 −554 234 −1211 599 172 487 −116 −990 −1644 −1036 100 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 101(E) −438 −1909 1578 1930 −2183 −946 37 −1928 199 −1929 −1110 415 −1271 427 −390 −260 509 −1512 −2154 −1401 101 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 102(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 102 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 103(I) −933 −617 −3067 −2622 −727 −2844 −2139 2601 −2382 283 375 −2419 −2785 −2186 −2438 −2063 −922 2174 −1934 −1553 103 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 104(E) 375 −1460 295 1605 −1753 −1028 168 −1444 819 −1486 −644 282 190 577 63 −106 −163 −1079 −1731 −1094 104 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 105(I) −486 −338 −2295 −1717 49 −2053 −856 1705 −1424 1267 888 −1489 −2030 −1121 −1416 −1138 −425 1093 −752 1040 105 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 106(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 106 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 107(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 107 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 108(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 108 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 109(M) −967 −770 −2707 −2179 −75 −2590 −1515 1740 −1841 1002 3350 −2043 −2485 −1555 −1846 −1742 −919 818 −1266 −1006 109 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 110(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 110 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 111(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 111 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 112(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 112 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 113(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 113 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 114(I) 1297 −396 −1045 −533 −555 −1309 −316 1303 −228 −265 313 −551 −1491 −201 106 −396 −102 374 −1004 −589 114 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 115(V) 433 −490 −819 416 −611 −1396 −259 391 −179 −21 288 −448 −1513 −95 −488 −437 −123 1568 −1055 −615 115 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 116(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 116 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 117(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 117 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 118(M) −825 −553 −2869 −2332 −347 −2619 −1636 2067 −2072 614 2211 −2123 −2524 −1766 −2066 −1762 −779 1949 −1423 −1130 118 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 119(S) 1087 −281 −851 −685 −1876 620 −774 −1551 −718 −1778 −951 −428 −1112 −546 −989 2116 79 −874 −2078 −1684 119 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 120(H) −303 −1577 31 461 −1878 −1155 1852 −1575 1230 −1543 −699 858 −1278 1692 489 −194 −226 −1208 −1701 −1118 120 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 121(M) −1179 −981 −2799 −2262 103 −2750 −1560 800 −1878 1813 3295 −2167 −2570 −1557 −1859 −1910 −1112 400 −1195 −971 121 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 122(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 122 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 123(V) −729 −571 −2495 −2034 1817 −2246 −1008 992 −1765 499 636 −1745 −2283 −1457 −1764 −1410 −714 2175 −737 −57 123 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 124(V) 1219 −338 −1791 −1461 −919 −1309 −1142 796 −1253 −361 23 −1160 −1724 −1114 −1391 −557 −284 2221 −1608 −1213 124 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 125(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 125 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 126(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 126 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 127(M) −964 −751 −2752 −2222 −95 −2613 −1544 1928 −1891 980 3191 −2075 −2502 −1594 −1889 −1765 −914 876 −1283 −1026 127 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 128(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 128 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 129(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 129 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 130(M) −415 −454 −1730 −1175 −135 −1824 −711 663 −895 1350 1822 −1122 −1866 −726 −1035 −898 1122 573 −924 −593 130 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 131(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 131 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 132(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 132 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 133(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 133 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 134(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 134 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 135(A) 2048 −367 −849 −680 −1659 −627 −739 −1190 −650 −1511 −785 −478 1606 −534 −899 74 −21 −698 −1911 −1515 135 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 136(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 136 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 137(R) −957 −1769 −1130 −344 −2309 −1642 117 −1834 1694 −1695 −991 −391 −1689 518 2806 −887 −779 −1577 −1692 −1419 137 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 138(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 138 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 139(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 139 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 140(S) 752 −403 −804 −361 −945 −914 −314 −429 −202 −754 1493 −350 −1267 −115 −505 1610 21 −179 −1285 −846 140 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 141(N) −187 −1264 170 440 −1640 −1019 83 −1319 462 −1408 −602 1650 −1249 1233 52 −142 1136 −974 −1676 −1083 141 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 142(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 142 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 143(C) 862 3529 −1939 −1540 −778 −1030 −977 437 −1267 −438 89 −1051 −1495 −1057 −1327 −271 −71 1212 −1336 −978 143 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 144(H) −1192 −1547 −812 −781 −520 −1557 4586 −1881 −457 −1789 −1357 −866 −1913 −669 −600 −1231 −1258 −1678 −918 −90 144 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 145(V) 635 −386 −1965 −1657 −921 −1492 −1321 997 −1438 −274 47 −1346 −1881 −1309 −1555 −752 −394 2522 −1700 −1297 145 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 146(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 146 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 147(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 147 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 148(I) −581 −428 −2332 −1778 −276 −2205 −1155 1757 −1518 1333 715 −1623 −2188 −1273 −1579 −1307 519 1349 −1155 −831 148 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 149(K) −594 −1483 −781 −58 −1796 −1470 168 −1358 2205 −248 −622 −193 −1513 562 1482 −556 −460 −1115 −1533 −1139 149 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 150(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 150 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 151(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 151 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 152(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 152 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 153(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 153 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 154(Q) −552 −1780 447 1123 −2047 −1104 22 −1774 497 −1764 −1012 208 −1382 2726 153 −415 −517 −1434 −1946 −1334 154 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 155(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 155 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 156(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 156 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 157(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 157 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 158(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 158 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 159(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 159 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 160(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 160 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 161(E) −704 −2274 1609 2494 −2517 −929 −118 −2327 −117 −2302 −1553 440 −1360 238 −777 −462 −729 −1891 −2491 −1671 161 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 162(A) 2429 −274 −996 −928 −1860 817 −956 −1435 −970 −1770 −1001 −575 −1171 −793 −1166 139 6 −817 −2094 −1749 162 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 163(A) 2297 −276 −974 −901 −1869 1169 −940 −1456 −949 −1779 −1003 −559 −1165 −770 −1154 146 12 −829 −2099 −1749 163 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 164(W) −599 −435 −2434 −1855 242 −2141 −835 1806 −1510 1433 984 −1586 −2094 −1181 −1459 −1234 −532 589 2084 −142 164 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 165(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 165 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 166(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 166 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 167(F) −889 −760 −2459 −2062 2900 −2246 −675 598 −1781 541 608 −1693 −2303 −1420 −1756 −1432 −885 1145 −260 608 167 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 168(D) −214 −1405 1586 576 −1945 −895 −13 −1678 183 −1732 −907 283 1136 366 −351 870 −261 −1244 −1978 −1306 168 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 169(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 169 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 170(Q) −552 −1780 447 1123 −2047 −1104 22 −1774 497 −1764 −1012 208 −1382 2726 153 −415 −517 −1434 −1946 −1334 170 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 171(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 171 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 172(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 172 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 173(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 173 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 174(V) −177 −366 −1747 −1376 −835 −1408 −1060 842 −1132 −278 103 −1145 −1767 −1025 −1282 −633 1362 2195 −1521 −1119 174 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 175(A) 2031 −283 −945 −876 −1891 1659 −933 −1488 −939 −1802 −1022 −547 −1163 −758 −1150 148 11 −850 −2114 −1762 175 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 176(I) −932 −615 −3069 −2624 −731 −2846 −2141 2579 −2386 277 371 −2421 −2787 −2189 −2441 −2065 −921 2203 −1937 −1555 176 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 177(A) 2214 −291 −1447 −1169 −1154 −884 −959 74 −982 −736 −262 −833 −1414 −858 −1151 −156 −103 1000 −1694 −1298 177 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 178(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 178 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 179(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 179 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 180(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 180 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 181(P) −370 −1016 −600 −377 −1687 −1102 −432 −1453 72 −1566 −902 −444 2899 −166 707 −467 −495 −1110 −1727 −1330 181 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 182(F) −1055 −829 −2811 −2263 2475 −2617 −1120 681 −1966 1473 2107 −2020 −2452 −1505 −1885 −1736 −975 308 −672 −125 182 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 183(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 183 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 184(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 184 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 185(I) −1143 −842 −3082 −2578 −159 −2929 −1882 2333 −2257 1719 917 −2424 −2743 −1919 −2225 −2119 −1091 1050 −1466 −1201 185 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 186(A) 2106 −237 −1021 −871 −1767 −506 −866 −1350 −841 −1674 −895 −527 −1137 −683 −1055 1564 68 −746 −2025 −1642 186 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 187(L) −1259 −980 −3063 −2554 2 −2960 −1832 1563 −2204 2258 1077 −2433 −2742 −1834 −2157 −2152 −1198 733 −1358 −1124 187 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 188(M) −1262 −1030 −2945 −2401 126 −2872 −1670 808 −2031 2152 2757 −2305 −2645 −1652 −1984 −2035 −1186 370 −1224 −1041 188 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 189(V) −851 −606 −2839 −2414 −730 −2622 −1965 1760 −2148 220 330 −2216 −2651 −1999 −2230 −1847 −862 2736 −1875 −1456 189 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 190(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 190 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 191(A) 1930 −244 −1003 −848 −1775 −507 −852 −1365 −819 −1681 −899 −516 −1135 −664 −1041 1787 68 −757 −2027 −1642 191 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 192(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 192 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 193(C) 763 1755 −1469 −973 −510 −1023 −517 135 −756 −355 310 −727 −1369 −559 −904 283 843 1335 −953 −582 193 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 194(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 194 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 195(R) 94 −1197 −868 −342 −1810 −1279 −102 −1370 814 −1456 −793 −379 −1529 246 2803 −535 −501 −1085 −1661 −1286 195 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 196(P) −92 −706 −669 −681 −2057 743 −909 −1842 −843 −2030 −1299 −596 3040 −724 −1088 −252 −384 −1257 −2095 −1813 196 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 197(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 197 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 198(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 198 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 199(L) −1198 −902 −3098 −2586 −74 −2954 −1866 1998 −2259 2016 1009 −2444 −2745 −1887 −2211 −2142 −1140 903 −1411 −1174 199 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 200(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 200 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 201(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 201 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 202(C) −608 5196 −2368 −2362 −1635 −1253 −1702 −987 −2034 −1540 −1191 −1745 −1831 −1953 −1922 −934 −927 −770 −1855 −1692 202 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 203(S) 1057 −274 −916 −815 −1832 −508 −865 −1436 −820 −1752 −977 −502 −1145 −674 −1039 2412 41 −810 −2079 −1679 203 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 204(V) −925 −616 −3000 −2497 −527 −2814 −1921 1840 −2252 949 560 −2316 −2703 −1995 −2284 −1988 −889 2430 −1695 −1364 204 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 205(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 205 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 206(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 206 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 207(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 207 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 208(T) −323 −1596 1187 1153 −1884 −973 55 −1567 265 −1645 −837 335 −1257 437 −273 −205 1701 −1205 −1920 −1239 208 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 209(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 209 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 210(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 210 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 211(E) −293 −1582 365 1545 −1897 −1040 140 −1597 1076 −1610 −776 280 −1253 546 110 939 −255 −1221 −1825 −1187 211 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 212(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 212 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 213(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 213 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 214(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 214 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 215(R) −664 −1241 −1101 −424 −1173 −1559 −60 −804 818 181 −343 −483 −1657 246 2582 −741 −578 −719 −1332 −877 215 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 216(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 216 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 217(F) −707 −668 −1832 −1360 2343 −1971 1711 −39 −1065 993 361 −1104 −1992 −805 −1140 −1047 −642 −73 294 1256 217 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 218(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 218 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 219(C) 1104 2197 −1323 −968 −1444 651 −781 −1064 −812 −1343 −561 −576 −1127 −634 −1023 1662 138 −550 −1740 −1381 219 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 220(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 220 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 221(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 221 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 222(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 222 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 223(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 223 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 224(I) −929 −611 −3073 −2627 −742 −2849 −2145 2502 −2391 262 361 −2423 −2789 −2195 −2447 −2067 −918 2293 −1944 −1560 244 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 225(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 255 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 226(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 226 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 227(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 227 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 228(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 228 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 229(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 229 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 230(E) −717 −2337 2049 2259 −2571 −918 −117 −2393 −138 −2352 −1602 461 −1357 240 −821 −464 −744 −1944 −2542 −1698 230 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 231(K) 397 −1331 0 518 −1619 −1070 250 −1306 1059 −1341 −481 786 38 674 793 224 −41 −946 −1579 −969 231 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 232(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 232 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 233(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 233 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 234(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 234 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 235(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 235 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 236(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 236 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 237(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 237 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 238(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 238 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 239(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 239 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 240(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 240 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 241(W) −1926 −1577 −2416 −2370 192 −2070 −795 −1602 −1882 −1347 −1210 −2018 −2383 −1854 −1777 −2150 −1992 −1643 5696 560 241 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 242(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 242 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 243(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 243 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 244(G) 1814 −291 −924 −870 −1911 1934 −940 −1512 −947 −1824 −1045 −544 −1165 −764 −1158 144 3 −867 −2131 −1779 244 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 245(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 245 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 246(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 246 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 247(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 247 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 248(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 248 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 249(C) 1738 3050 −1597 −1286 −1304 −553 −920 −809 −1074 −1196 −460 −726 −1161 −870 −1191 1014 128 −366 −1662 −1327 249 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 250(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 250 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 251(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 251 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 252(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 1992 −1530 252 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 253(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 253 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 254(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 254 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 255(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 255 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 256(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 256 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 257(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 257 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 258(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 258 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 259(F) −1868 −1403 −2783 −2661 3327 −2745 337 −941 −2325 −619 −501 −1662 −2699 −1613 −2087 −1932 −1788 −1044 1008 2959 259 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 260(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 260 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 261(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 261 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 262(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 262 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 263(S) 1057 −279 −958 −668 −1633 −564 −673 −1214 −584 −1503 −711 −437 −1133 −450 −856 1823 1396 −673 −1872 −1473 263 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 264(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 264 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 265(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 265 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 266(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 266 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 267(A) 1795 −308 −1596 −1269 −985 −1098 −1001 487 −1073 −504 −67 −970 −1559 −938 −1234 −348 −181 1695 −1593 −1199 267 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 268(M) −485 −617 −1471 −910 −110 −1822 −557 403 −543 1390 2375 −948 −1827 805 −720 −871 −418 228 −912 −567 268 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 269(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 269 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 270(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 270 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 271(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 271 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 272(A) 1700 −251 −1028 −731 −1556 −572 −693 −1113 −631 −1425 −647 −472 −1141 −490 −887 934 1645 −603 −1818 −1429 272 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 273(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 273 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 274(G) 307 −371 −641 −650 −1997 2231 −857 −1752 −837 −1971 −1163 −431 −1164 −654 −1088 1356 −32 −1032 −2185 −1778 274 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 275(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 275 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 276(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 276 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 277(M) −965 −982 −2125 −1844 −276 −2022 −1302 373 −1390 610 4141 −1701 −2229 −1356 −1425 −1444 −1028 126 −1279 −880 277 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 278(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 278 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 279(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 279 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 280(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 280 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 281(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 281 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 282(A) 1942 −244 −1004 −849 −1775 −507 −853 −1364 −820 −1681 −899 −516 −1135 −665 −1041 1773 68 −756 −2027 −1641 282 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 283(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 283 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 284(C) −608 5196 −2368 −2362 −1635 −1253 −1702 −987 −2034 −1540 −1191 −1745 −1831 −1953 −1922 −934 −927 −770 −1855 −1692 284 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 285(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 285 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 286(Y) −1934 −1442 −2813 −2708 2952 −2777 363 −1027 −2370 −696 −580 −1669 −2726 −1633 −2118 −1965 −1850 −1121 1040 3463 286 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 287(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 287 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 288(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 288 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 289(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 289 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 290(A) 2297 −276 −974 −901 −1869 1169 −940 −1456 −949 −1779 −1003 −559 −1165 −770 −1154 146 12 −829 −2099 −1749 290 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 291(A) 2106 −237 −1021 −871 −1767 −506 −866 −1350 −841 −1674 −895 −527 −1137 −683 −1055 1564 68 −746 −2025 −1642 291 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 292(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 292 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 293(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 293 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 294(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 294 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 295(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 295 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 296(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 296 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 297(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 297 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 298(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 298 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 299(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 299 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 300(S) 1375 −276 −871 −686 −1859 1354 −765 −1534 −710 −1758 −930 −428 −1109 −536 −985 1511 86 −862 −2062 −1669 300 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 301(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 301 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 302(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 302 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 303(C) −608 5196 −2368 −2362 −1635 −1253 −1702 −987 −2034 −1540 −1191 −1745 −1831 −1953 −1922 −934 −927 −770 −1855 −1692 303 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 304(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 304 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 305(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 305 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 306(M) −837 −618 −2708 −2158 −178 −2529 −1480 1612 −1882 830 3103 −1991 −2434 −1567 −1889 −1657 −786 1490 −1279 −1018 306 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 307(P) 180 −461 −726 −522 −1558 −715 −605 −1079 −438 −1388 −684 −414 2065 −378 −702 3 1812 −655 −1805 −1384 307 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 308(G) 293 −379 −630 −667 −2009 2397 −884 −1772 −875 −1997 −1196 −444 −1174 −691 −1115 1071 −51 −1049 −2198 −1793 308 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 309(A) 2390 −226 −1055 −939 −1760 −504 −915 −1313 −910 −1669 −905 −561 −1146 −752 −1102 1042 58 −721 −2034 −1657 309 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 310(V) −836 −639 −2662 −2225 −512 −2495 −1702 1367 −1909 852 487 −2046 −2529 −1758 −1977 −1708 −841 2598 −1613 −1195 310 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 311(P) 2 −489 −1031 −785 −1108 −956 −714 −305 −612 −789 −314 −653 2544 −567 −828 −238 −203 890 −1569 −1119 311 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 312(S) 311 −370 −673 −637 −1958 759 −820 −1708 −773 −1924 −1114 −425 −1160 −607 −1031 2407 −18 −1005 −2145 −1732 312 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 313(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 313 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 314(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 314 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 315(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 315 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 316(A) 1567 −924 62 917 −1556 −884 −105 −1178 129 −1361 −576 63 −1226 230 −299 637 −121 −810 −1700 −1148 316 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 317(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 317 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 318(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 318 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 319(G) 1867 −289 −929 −871 −1906 1873 −938 −1506 −944 −1818 −1039 −545 −1165 −761 −1155 146 5 −862 −2126 −1774 319 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 320(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 320 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 321(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 321 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 322(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 322 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 323(I) −1123 −822 −3067 −2569 −197 −2917 −1887 2447 −2248 1582 875 −2413 −2743 −1929 −2225 −2110 −1075 1105 −1492 −1210 323 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 324(C) 1325 3551 −1712 −1385 −1146 −650 −942 −408 −1136 −968 −296 −812 −1233 −935 −1223 78 996 −79 −1561 −1227 324 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 325(M) 79 −386 −845 −374 −744 −1030 −267 −221 −185 −543 2172 −382 −1318 −104 −470 1220 702 −27 −1118 −688 325 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 326(M) 433 −323 −1005 −574 −677 −1039 −385 −108 −368 −444 2884 −511 −1361 −278 −604 837 −22 63 −1103 −689 326 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 327(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 327 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 328(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 328 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 329(L) −1060 −771 −3035 −2500 −172 −2852 −1816 1635 −2228 1939 929 −2342 −2674 −1854 −2200 −2012 −1004 1515 −1444 −1221 329 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 330(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 330 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 331(C) −206 3367 −2319 −1919 −681 −1494 −1270 951 −1603 −153 201 −1455 −1861 −1411 −1625 −746 −346 2000 −1422 −1027 331 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 332(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 332 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 333(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 333 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 334(A) 2078 −244 −950 −773 −1815 720 −810 −1474 −782 −1719 −902 −471 −1112 −603 −1033 1059 91 −820 −2040 −1661 334 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 335(N) 73 2062 −715 −459 −1269 −837 −491 −919 −332 −1269 −568 2714 −1301 −294 −604 −98 −125 −565 −1556 −1049 335 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 336(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 336 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 337(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 337 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 338(T) 1532 −257 −1123 −898 −1523 −620 −822 −803 −773 −1312 −629 −583 −1207 −654 −978 96 2243 −396 −1854 −1476 338 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 339(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 339 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 340(S) 337 −317 −860 −701 −1734 −558 −753 −1328 −649 −1636 −869 −457 −1159 −543 −888 2315 1114 −760 −1977 −1560 340 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 341(H) −1192 −1547 −812 −781 −520 −1557 4586 −1881 −457 −1789 −1357 −866 −1913 −669 −600 −1231 −1258 −1678 −918 −90 341 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 342(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 342 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 343(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 343 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 344(M) −962 −721 −2832 −2307 −165 −2664 −1626 2366 −1987 903 2608 −2145 −2549 −1688 −1979 −1822 −914 1020 −1353 −1088 344 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 345(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 345 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 346(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 346 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 347(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 347 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 348(A) 1597 −978 151 960 −1634 273 −124 −1266 89 −1438 −652 85 −1234 211 −352 −94 −159 −886 −1767 −1206 348 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 349(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 349 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 350(M) −441 −455 −1810 −1252 −103 −1867 −749 679 −965 1297 2343 −1183 −1898 −782 −1087 −944 877 573 −915 −594 350 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 351(L) −1259 −980 −3063 −2554 2 −2960 −1832 1563 −2204 2258 1077 −2433 −2742 −1834 −2157 −2152 −1198 733 −1358 −1124 351 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 352(M) −73 −469 −1004 −675 −744 1014 −512 −220 −464 −456 3139 −614 −1458 −414 −675 −331 −188 −84 −1209 −778 352 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 353(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 353 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 354(M) −997 −819 −2602 −2110 2400 −2453 −884 573 −1803 986 2860 −1838 −2371 −1404 −1756 −1593 −939 253 −467 230 354 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 355(L) −1198 −902 −3098 −2586 −74 −2954 −1866 1998 −2259 2016 1009 −2444 −2745 −1887 −2211 −2142 −1140 903 −1411 −1174 355 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 356(P) 1545 −393 −808 −668 −1689 −637 −750 −1218 −653 −1539 −824 −478 2324 −545 −899 53 −48 −726 −1936 −1539 356 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 357(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 357 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 358(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 358 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 359(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 359 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 360(F) −1902 −1421 −2805 −2692 3214 −2763 351 −984 −2354 −657 −540 −1668 −2714 −1627 −2106 −1950 −1820 −1082 1026 3156 360 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 361(I) −1076 −780 −3002 −2529 −309 −2874 −1898 2697 −2198 1168 740 −2371 −2742 −1949 −2205 −2081 −1040 1240 −1569 −1227 361 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 362(F) −151 −497 −1152 −820 2062 −1206 −264 −329 −685 −526 −9 −681 −1538 −520 −889 1555 −260 −181 −431 409 362 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 363(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 363 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 364(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 364 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 365(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 365 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 366(S) 1123 −271 −926 −817 −1828 −508 −863 −1430 −818 −1746 −969 −502 −1143 −671 −1038 2382 45 −805 −2074 −1676 366 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 367(A) 2139 −236 −1024 −877 −1766 −505 −870 −1347 −846 −1673 −895 −530 −1137 −689 −1059 1515 68 −744 −2025 −1643 367 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 368(V) −151 −569 −631 524 −788 −1293 −269 234 −134 −481 122 −371 −1478 −70 −451 −389 637 1708 −1201 −747 368 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 369(P) −478 −1420 327 1138 −1929 −1032 −248 −1622 −3 −1747 −1039 36 2562 70 −402 −436 −543 −1278 −1965 −1426 369 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 370(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 370 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 371(Y) −806 −794 −1826 −1397 917 −2006 −90 −29 −1025 35 1798 −1154 −2057 −849 −1103 −1127 −760 −95 211 3271 371 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 372(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 372 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 373(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 373 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 374(M) −204 −445 −1398 −949 −453 −1403 −637 369 −636 129 3007 −865 −1648 −574 −809 −563 1278 377 −1116 −731 374 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 375(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 375 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 376(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 376 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 377(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 377 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 378(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 378 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 379(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 379 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 380(M) −79 −344 −907 352 1390 −1364 −137 182 −190 −87 1450 −439 −1442 −63 −461 −376 598 278 −733 −299 380 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 381(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 381 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 382(A) 1918 −240 −1121 −794 −1248 520 −675 −589 −672 −1071 −369 −547 −1218 −526 −907 65 56 766 −1598 −1207 382 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 383(E) −411 −1179 −46 1823 −1097 −1265 −120 −540 187 648 −221 −83 −1466 183 −172 −447 −380 −443 −1459 −905 383 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 384(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 384 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 385(F) −1902 −1421 −2805 −2692 3214 −2763 351 −984 −2354 −657 −540 −1668 −2714 −1627 −2106 −1950 −1820 −1082 1026 3156 385 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 386(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 386 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 387(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 387 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 388(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 388 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 389(N) −199 −701 −549 −93 −228 −1316 21 533 64 −536 109 1814 −1432 121 −268 −348 −154 −118 −681 1083 389 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 390(V) −596 −427 −2444 −1942 −648 −2270 −1439 1955 −1702 78 364 −1768 −2320 −1523 −1801 −1418 532 2258 −1522 −1137 390 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 391(M) −1151 −859 −3123 −2554 25 −2901 −1760 1894 −2267 1875 2113 −2393 −2661 −1798 −2177 −2046 −1074 735 −1288 −1136 391 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 392(Q) −513 −1819 522 1560 −2087 −1069 43 −1821 462 −1801 −1025 265 −1348 2403 72 −362 −480 −1458 −1986 −1342 392 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 393(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 393 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 394(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 394 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 395(L) −1306 −1067 −2981 −2443 125 −2920 −1717 814 −2062 2337 2190 −2353 −2682 −1686 −2014 −2092 −1231 368 −1248 −1063 395 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 396(M) 872 −604 −643 −123 −730 −1240 −67 −190 798 −479 2200 −252 −1378 160 −90 −281 −76 −42 −1061 −617 396 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 397(V) −916 −603 −3047 −2601 −754 −2826 −2126 2255 −2365 237 345 −2398 −2775 −2177 −2427 −2043 −906 2508 −1944 −1553 397 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 398(D) −673 −2181 2496 794 −2461 −904 −143 −2337 −175 −2319 −1575 1933 −1355 203 −844 −448 −722 −1882 −2482 −1647 398 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 399(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 399 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 400(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 400 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 401(L) −1198 −902 −3098 −2586 −74 −2954 −1866 1998 −2259 2016 1009 −2444 −2745 −1887 −2211 −2142 −1140 903 −1411 −1174 401 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 402(R) −345 −1126 −591 42 −1183 −1345 1446 −901 819 −996 1243 −126 −1412 479 1981 −372 −253 −690 −1261 −760 402 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 403(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 403 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 404(V) −886 −604 −2944 −2505 −743 −2731 −2045 1944 −2256 229 340 −2308 −2716 −2086 −2330 −1950 −884 2675 −1910 −1506 404 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 405(T) −79 −1241 710 507 −1575 −1016 183 −1259 542 −1330 −485 230 −1179 590 737 676 1004 −901 −1599 −987 405 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 406(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 406 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 407(E) 39 −1704 551 2063 −1995 −1015 71 −1696 401 −1718 −915 313 −1287 1274 −58 −256 −368 −1326 −1952 −1286 407 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 408(E) −414 −1855 1337 2003 −2133 −952 45 −1867 223 −1879 −1060 402 −1266 436 −354 −246 592 −1459 −2110 −1370 408 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 409(V) −82 −237 −1362 −829 −406 −1408 −463 1109 −622 −139 448 −766 −1572 −466 −821 288 937 1440 −906 −511 409 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 410(I) −1016 −805 −2637 −2367 −591 −2470 −1853 3039 −2040 329 346 −2180 −2589 −1943 −2070 −1879 −1051 1184 −1698 −1261 410 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 411(K) 1191 −905 −299 140 −1432 −1011 −7 −980 1412 −1186 −412 −29 −1257 344 101 −104 626 −667 −1531 −1026 411 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 412(I) 442 −488 −2154 −1781 −680 −1947 −1383 2623 −1524 86 307 −1582 −2159 −1402 −1631 −1169 −575 1239 −1576 −1178 412 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 413(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 413 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 414(N) −406 −1604 364 514 −1650 −1088 1550 −1590 512 −1604 −816 2266 −1324 905 133 −295 −367 −1249 −1692 −1010 414 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 415(K) −1028 −1686 −800 −442 −2171 −1521 −263 −1873 3103 −1859 −1251 −547 −1750 74 599 −1004 −972 −1620 −1812 −1528 415 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 416(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 416 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 417(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 417 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 418(R) −426 −1022 −965 −360 −1251 −1374 −109 −634 663 −894 −340 −418 −1549 198 2485 −538 −408 540 −1406 −969 418 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 419(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 419 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 420(I) −1039 −725 −3101 −2580 −296 −2896 −1918 2322 −2317 1504 807 −2405 −2727 −1971 −2296 −2068 −990 1488 −1558 −1305 420 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 421(Q) −862 −1548 −321 −247 −1672 −1336 −419 −1721 130 −1660 −1161 −427 −1678 3540 −59 −855 −904 −1495 −1728 −1237 421 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 422(A) 2439 −274 −998 −931 −1859 786 −957 −1433 −972 −1769 −1001 −577 −1172 −795 −1167 139 6 −816 −2094 −1749 422 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 423(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 423 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 424(F) −1602 −1340 −2512 −2455 3740 −2315 −379 −478 −2225 −175 −190 −1874 −2503 −1781 −2079 −1892 −1647 −683 210 1257 424 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 425(E) 394 −1564 893 1329 −1847 −1017 212 −1570 575 −1566 −695 332 −1188 639 992 −66 −138 −1170 −1778 −1108 425 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 426(E) −547 −2038 1492 1544 −2334 1402 −46 −2115 24 −2100 −1309 431 −1308 325 −605 −346 −562 −1682 −2320 −1536 426 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 427(L) −1304 −1065 −2981 −2443 126 −2919 −1716 814 −2063 2331 2215 −2353 −2680 −1685 −2014 −2091 −1230 368 −1247 −1063 427 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 428(G) −330 −1125 −91 −18 −1933 2258 −329 −1639 1029 −1762 −1028 −173 −1409 −22 −158 −369 −453 −1229 −1912 −1465 428 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 429(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 429 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 430(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 430 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 431(Q) 337 −1173 −102 413 −1398 −1116 203 −1043 1032 −735 −333 153 727 1159 238 −88 −61 −748 −1447 −885 431 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 432(I) −938 −664 −2954 −2530 −626 −2743 −2041 2838 −2253 397 449 −2334 −2724 −2070 −2306 −1976 −939 1704 −1845 −1460 432 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 433(T) 737 −257 −1006 −798 −1590 −576 −766 −1064 −703 −1448 −698 −511 −1166 −583 −923 595 2548 −574 −1871 −1487 433 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 434(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 434 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 435(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 435 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 436(E) −988 −1921 379 3052 −2309 −1196 −495 −2108 −350 −2194 −1612 −103 −1621 −210 −770 −862 −1042 −1799 −2207 −1742 436 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 437(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 437 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 438(E) −462 −1877 1098 2281 −2164 −948 4 −1884 157 −1917 −1119 395 −1290 383 −418 −292 231 −1485 −2158 −1417 438 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 439(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 439 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 440(A) 2214 −291 −1447 −1169 −1154 −884 −959 74 −982 −736 −262 −833 −1414 −858 −1151 −156 −103 1000 −1694 −1298 440 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 441(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 441 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 442(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 442 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 443(A) 2125 −240 −956 −787 −1817 725 −821 −1474 −799 −1722 −906 −477 −1113 −618 −1045 968 90 −818 −2044 −1667 443 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 444(H) −241 −1271 205 405 −1420 −1027 2877 −1354 381 −1435 −651 1012 −1286 387 −16 526 −247 −1015 −1560 −893 444 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 445(G) 115 −688 −127 −9 −1770 1729 −357 −1446 −174 −1623 −822 1088 −1193 −53 −556 21 909 −938 −1917 −1403 445 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 446(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 446 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 447(K) −546 −1647 −8 263 −2054 −1224 81 −1743 2289 −1711 −938 1349 −1428 473 604 −449 −488 −1398 −1805 −1294 447 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 448(D) −824 −2383 2964 1142 −2628 −923 −210 −2461 −314 −2455 −1761 430 −1405 123 −1021 −562 −876 −2027 −2629 −1790 448 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 449(M) −764 −700 −2351 −1867 −159 −2218 −1306 906 −1516 830 3512 −1724 −2266 −1323 −1574 −1392 −764 1232 −1247 −932 449 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 450(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 450 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 451(E) 99 −1520 298 1621 −1816 −1039 179 −1514 1042 −1533 −686 287 196 594 120 −117 −179 −1138 −1759 −1119 451 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 1378 * * 452(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 452 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 453(N) −607 −1987 1547 723 −2300 −917 −146 −2176 −128 −2194 −1447 2758 −1353 195 −745 −420 −660 −1739 −2343 −1556 453 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 454(M) −798 −549 −2776 −2235 −304 −2552 −1547 1726 −1970 646 2482 −2039 −2467 −1670 −1974 −1687 −751 2029 −1361 −1070 454 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 455(V) −700 −665 −2337 −2135 −873 −1965 −1727 1107 −1863 −105 22 −1851 −2286 −1794 −1920 −1368 −823 2927 −1816 −1362 455 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 456(E) −565 −1912 661 2316 −2164 −1033 −4 −1917 326 −1900 −1139 305 −1356 1326 −107 −396 −548 −1547 −2092 −1413 456 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 457(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 457 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 458(M) −1168 −891 −3079 −2505 76 −2883 −1714 1503 −2212 1892 2710 −2358 −2638 −1740 −2126 −2022 −1087 602 −1243 −1101 458 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 459(K) 182 −1171 −183 203 −1755 −1060 40 −1376 2110 −1461 −666 13 −1300 422 353 563 −217 −1012 −1691 −1179 459 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 460(A) 1995 −401 −1320 −961 1082 −1166 −569 41 −802 −291 135 −791 −1531 −642 −991 −375 −205 143 −927 −304 460 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 461(A) 1982 −301 −1528 −1215 −1040 −1011 −971 334 −1023 −585 −132 −907 −1498 −891 −1190 −268 −147 1432 −1620 −1225 461 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 462(Q) −508 −1830 541 1702 −2097 −1060 45 −1834 449 −1813 −1033 277 −1342 2266 48 −354 −475 −1466 −1998 −1347 462 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 463(E) −565 −2113 1507 2151 −2387 321 −37 −2180 33 −2146 −1350 447 −1307 338 −604 −350 −576 −1736 −2355 −1553 463 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 464(M) −1145 −875 −3042 −2468 69 −2853 −1685 1502 −2175 1789 2881 −2322 −2619 −1717 −2097 −1990 −1065 616 −1235 −1085 464 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 465(M) −964 −754 −2746 −2216 −92 −2609 −1540 1901 −1884 984 3216 −2071 −2499 −1589 −1883 −1761 −915 868 −1280 −1023 465 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 466(N) −212 −1586 953 652 −1877 −1008 188 −1602 1202 −1601 −736 1422 −1199 610 33 −89 555 −1202 −1814 −1139 466 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 467(R) −957 −1779 −1134 −338 −2324 −1645 124 −1844 1755 −1699 −992 −386 −1687 527 2771 −884 −776 −1586 −1694 −1422 467 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 468(N) −145 −1016 214 150 −2012 1688 −401 −1836 −268 −1957 −1185 2176 −1294 −105 −680 −181 −340 −1299 −2102 −1535 468 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 469(I) −462 −597 −1498 −959 −506 −1717 −547 2312 −237 −2 349 −933 −1823 −455 761 −864 −432 572 −1103 −678 469 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 470(T) 49 −672 −270 −138 −1544 −795 −385 −1139 −160 −1431 −698 1092 −1260 −119 −475 −71 2357 −741 −1759 −1248 470 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 471(G) 164 −379 −1029 −881 −1405 2294 −838 −705 −813 −1237 −627 −637 −1312 −705 −1013 −66 −112 840 −1767 −1349 471 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 472(V) −973 −676 −3010 −2484 −310 −2805 −1822 1723 −2225 1595 781 −2304 −2660 −1894 −2215 −1965 −925 1960 −1517 −1250 472 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 473(D) −792 −2412 2801 1455 −2641 −913 −169 −2475 −253 −2443 −1727 459 −1383 174 −964 −525 −834 −2028 −2631 −1771 473 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 474(V) −924 −606 −3067 −2621 −751 −2843 −2142 2385 −2386 247 351 −2416 −2786 −2193 −2444 −2061 −913 2407 −1948 −1560 474 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 475(V) −886 −604 −2944 −2505 −743 −2731 −2045 1944 −2256 229 340 −2308 −2716 −2086 −2330 −1950 −884 2675 −1910 −1506 475 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 476(K) −494 −1536 −395 133 −1991 −6 152 −1602 2266 −1562 −774 −53 −1428 560 1152 −424 −406 −1276 −1682 −1238 476 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 477(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 477 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 478(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 −488 −1867 2067 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 478 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 479(Y) 499 −364 −899 −371 787 −1296 −82 7 −211 −277 393 −419 −1419 −73 −488 927 −30 144 −587 1023 479 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 480(K) −84 −1454 654 613 −1738 −1039 285 −1458 1165 −1450 −558 861 −1146 994 487 248 −23 −503 −1655 −1002 480 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 481(H) −138 −1185 181 434 −1561 117 2574 −1298 368 −1392 −582 681 −1230 426 −73 519 −152 −942 −1647 −1037 481 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 482(G) −478 −1458 1461 350 −2273 2341 −418 −2092 −439 −2185 −1451 76 −1399 −120 −981 −425 −627 −1604 −2284 −1711 482 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 483(F) −1075 −853 −2688 −2263 2906 −2522 −760 1338 −1963 741 748 −1891 −2464 −1545 −1898 −1690 −1030 450 −271 583 483 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 484(K) −186 −1612 1105 1050 −1898 −1008 220 −1635 1350 −1612 −730 353 −372 652 63 −56 254 −1218 −1811 −1126 484 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 485(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 485 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 486(V) −913 −603 −3038 −2592 −754 −2818 −2119 2212 −2355 235 344 −2390 −2769 −2168 −2418 −2035 −904 2536 −1941 −1549 486 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 487(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 487 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 488(Q) −287 −1670 472 1418 −1956 −1010 152 −1686 494 −1676 −825 781 −1230 1735 6 468 −249 −1287 −1883 −1205 488 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 489(N) 982 −1511 1230 624 −1958 −918 1 −1675 186 −1740 −924 1829 −1246 379 −358 −177 −306 −1265 −1993 −1307 489 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 490(I) −844 −558 −2919 −2391 −410 −2672 −1718 2212 −2137 556 1861 −2182 −2573 −1841 −2137 −1823 −801 2017 −1502 −1199 490 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 491(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 491 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 492(N) −436 −1887 1302 728 −2163 −972 57 −1926 969 −1904 −1081 2205 −1277 450 −237 −263 −420 −1510 −2102 −1373 492 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 493(M) −1145 −875 −3042 −2468 69 −2853 −1685 1502 −2175 1789 2881 −2322 −2619 −1717 −2097 −1990 −1065 616 −1235 −1085 493 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 494(Q) 438 −738 −549 −83 −752 −1276 −87 −244 206 754 150 −239 −1416 1553 −92 −333 −154 −140 −1136 −668 494 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 495(K) −768 −1791 −831 −81 −2307 −1536 199 −1845 2218 −1687 −922 −205 −1567 1413 1839 −665 −601 −1541 −1705 −1361 495 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 496(Q) 607 −1101 −64 144 −1616 −1017 −115 −1247 345 −1377 −685 −39 −1340 2700 48 −248 −291 −931 −1715 −1175 496 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 497(K) −930 −1812 −1098 −282 −2373 −1635 152 −1883 2344 −1718 −994 −346 −1664 564 2242 −842 −743 −1611 −1705 −1425 497 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 498(V) −925 −616 −3000 −2497 −527 −2814 −1921 1840 −2252 949 560 −2316 −2703 −1995 −2284 −1988 −889 2430 −1695 −1364 498 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 499(C) 375 2958 −1479 −1118 −1141 −638 −785 −629 −894 −1021 −301 −681 −1196 −724 −1042 842 1871 −256 −1509 −1153 499 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 500(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 500 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 501(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 501 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 502(Y) −1894 −1443 −2713 −2588 2338 −2734 353 −1021 −2261 −702 −584 −1632 −2696 −1582 −2053 −1924 −1818 −1110 1018 3836 502 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 503(L) −1369 −1199 −2542 −2295 −129 −2453 −1603 488 −1867 2607 742 −2168 −2539 −1724 −1835 −1992 −1384 130 −1284 −897 503 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 504(Q) −528 −1553 −18 249 −1355 −1246 1676 −1537 686 −1512 −787 17 −1438 2674 436 −447 −468 −1245 −1443 −765 504 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 505(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 505 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 506(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 506 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 507(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 507 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 508(I) −938 −642 −3015 −2577 −673 −2799 −2092 2768 −2321 349 419 −2380 −2757 −2128 −2375 −2024 −932 1888 −1889 −1511 508 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 509(F) −1021 −751 −2984 −2430 1835 −2690 −1365 1831 −2140 1558 1076 −2171 −2517 −1668 −2032 −1822 −947 667 −916 −487 509 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 510(D) −228 −933 1676 139 −980 −1247 −106 −126 97 59 −65 −98 −1412 196 −332 −320 −202 651 −1373 −816 510 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 511(K) −125 −1470 735 601 −1768 −117 251 −1483 1009 −1480 −601 299 −1169 685 990 468 −71 −1087 −1689 −1041 511 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 512(Q) −215 −1686 744 1127 −1974 −31 209 −1725 1008 −1685 −799 954 −1185 1224 24 −72 −169 −1293 −1872 −1170 512 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 513(W) −590 −560 −1824 −1369 2316 64 40 −143 −1101 −278 223 −1055 −1904 −810 −1154 −929 −548 −80 3223 1325 513 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 514(H) −169 −1619 601 1061 −1918 −1017 1341 −1660 1041 −1619 −725 901 −1171 1254 132 −37 −116 −1232 −1801 −1117 514 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 515(V) −337 1937 −2456 −2043 −721 −1722 −1414 1163 −1743 −109 212 −1622 −2030 −1552 −1771 −966 −446 2551 −1519 −1120 515 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 516(V) −571 −393 −2384 −1841 −446 −2249 −1248 1704 −1594 738 539 −1678 −2244 −1375 −1669 −1360 519 2046 −1284 −924 516 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 517(S) 116 −498 −840 −867 −1751 −671 −949 −1625 −916 −1896 −1218 −622 −1304 −824 −1097 2786 −220 −1038 −1992 −1530 517 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 518(A) 2806 −444 −1270 −1265 −1732 −744 −1182 −1034 −1212 −1545 −1010 −869 −1380 −1102 −1325 −156 −259 −640 −2039 −1721 518 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 519(V) −886 −604 −2944 −2505 −743 −2731 −2045 1944 −2256 229 340 −2308 −2716 −2086 −2330 −1950 −884 2675 −1910 −1506 519 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 520(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 520 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 521(D) −710 −2209 2783 792 −2489 −901 −175 −2383 −242 −2372 −1646 1391 −1370 162 −925 −480 −769 −1927 −2525 −1680 521 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 522(I) 926 1569 −2066 −1523 −360 −1740 −859 1653 −1268 36 522 −1286 −1866 −1039 −1329 −859 −244 1499 −985 −613 522 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 523(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 523 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 524(D) −1130 −2063 3441 243 −2502 −1157 −662 −2514 −843 −2578 −2029 −96 −1658 −421 −1434 −970 −1239 −2138 −2388 −1915 524 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 525(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 525 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 526(Q) 394 −1108 −203 338 −1300 −1159 184 −918 1042 −1043 968 94 −1255 1737 277 −133 −76 −656 −1380 −845 526 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 527(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 527 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 528(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 528 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 529(G) 1163 −332 −849 −861 −1983 2434 −981 −1591 −996 −1909 −1142 −553 −1190 −813 −1198 105 −47 −932 −2188 −1842 529 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 530(T) −52 −555 −1173 −1112 −1571 −872 −1041 −862 −950 −1353 −866 −843 −1455 −937 −1084 −285 3069 −557 −1898 −1539 530 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 531(G) −758 −1103 −1313 −1487 −2460 3331 −1562 −2502 −1720 −2644 −2081 −1324 −1775 −1590 −1790 −958 −1093 −1921 −2198 −2279 531 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 532(Y) −1667 −1473 −2001 −1964 1181 −2210 −25 −1224 −1658 −1018 −871 −1505 −2415 −1423 −1618 −1747 −1692 −1248 501 4149 532 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 533(R) −1181 −1614 −1431 −903 −2066 −1632 −446 −1944 559 −1867 −1326 −899 −1873 −156 3432 −1225 −1148 −1717 −1746 −1529 533 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 534(L) −1227 −936 −3091 −2579 −37 −2962 −1855 1807 −2244 2137 1045 −2445 −2745 −1867 −2194 −2151 −1166 827 −1387 −1156 534 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 535(W) −110 −1096 −68 835 −1238 −1114 184 −918 540 −1049 −251 146 −1227 1144 118 925 −52 −647 1432 −790 535 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 536(E) 374 −1428 234 1314 −1721 −59 206 −1425 962 −1455 −596 292 −335 625 87 −51 −101 −1049 −1693 −1050 536 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −660 −6045 −1506 −894 −1115 −701 −1378 * * 537(D) −431 −1310 2676 692 −1656 −655 −71 −1520 −129 −1690 −1117 380 −1112 179 −645 −333 −523 −1206 −1668 −1155 537 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −50 −5435 −6477 −894 −1115 −1219 −810 * * 538(K) −15 −765 −73 241 −1227 −873 152 −678 1382 −945 −263 98 −1144 476 501 −77 963 −426 −1324 −852 538 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −50 −5435 −6477 −894 −1115 −451 −1896 * * 539(E) −567 −2146 1487 2236 −2383 −935 −11 −2178 98 −2131 −1330 451 −1305 974 −516 −347 −566 −1739 −2332 −1532 539 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 540(R) −635 −1728 −475 809 −2174 −1411 191 −1754 1549 −1647 −864 −79 −1485 610 2276 −530 −501 −1435 −1716 −1303 540 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 541(W) −1926 −1577 −2416 −2370 192 −2070 −795 −1602 −1882 −1347 −1210 −2018 −2383 −1854 −1777 −2150 −1992 −1643 5696 560 541 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 542(E) 376 −1973 1778 1849 −2250 −935 10 −2003 135 −2000 −1190 428 −1283 394 −473 −289 −479 −1580 −2225 −1454 542 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 543(E) −502 −1664 66 1892 −2010 −1207 100 −1682 762 −1657 −874 90 −1392 499 1653 −394 −434 −1344 −1798 −1268 543 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 544(I) −1076 −780 −3002 −2529 −309 −2874 −1898 2697 −2198 1168 740 −2371 −2742 −1949 −2205 −2081 −1040 1240 −1569 −1227 544 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 545(K) −636 −1780 35 978 −2175 −1268 89 −1797 2425 −1748 −984 44 −1457 488 647 −514 −552 −1469 −1853 −1366 545 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 546(N) −637 −1349 −5 −148 −1773 −1078 −624 −1965 −499 −2112 −1514 3456 −1574 −435 −810 −661 −818 −1560 −1900 −1327 546 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 547(I) −985 −659 −3098 −2595 −475 −2895 −1995 2517 −2348 1018 628 −2411 −2755 −2059 −2358 −2076 −945 1794 −1699 −1395 547 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 548(P) 1061 −433 −770 −684 −1731 −657 −791 −1256 −689 −1580 −892 −499 2706 −594 −921 8 −100 −769 −1976 −1581 548 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 549(W) −38 −416 −726 −186 608 365 −32 −47 −33 −324 375 490 −1365 81 −348 −269 377 100 1582 −231 549 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 550(A) 1982 −301 −1528 −1215 −1040 −1011 −971 334 −1023 −585 −132 −907 −1498 −891 −1190 −268 −147 1432 −1620 −1225 550 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 551(M) −956 −675 −3000 −2437 −98 −2720 −1640 1822 −2169 1596 1921 −2230 −2553 −1752 −2099 −1856 −891 1429 −1284 −1077 551 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 552(D) −409 −1868 1976 711 −2139 −994 95 −1893 949 −1858 −1023 375 −1268 1572 −112 −239 −379 −1478 −2048 −1334 552 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 553(P) −934 −1220 −1352 −1451 −2187 −1314 −1456 −2160 −1488 −2268 −1827 −1359 3760 −1471 −1571 −1129 −1216 −1780 −2050 −2023 553 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 554(Q) −349 −1867 1242 1165 −2138 −348 120 −1907 361 −1863 −1004 1155 −1230 1761 −205 −176 −320 −1469 −2057 −1314 554 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 555(E) −176 −1613 862 1081 −1899 −1001 224 −1641 1004 −1615 −729 978 −1172 658 47 503 231 −1220 −1813 −1122 555 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 556(I) −1082 −793 −3068 −2508 −48 −2847 −1738 2225 −2221 1561 2060 −2337 −2638 −1795 −2152 −1992 −1013 884 −1319 −1134 556 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −33 −6045 −7087 −894 −1115 −701 −1378 * * 557(E) −271 −1743 826 1604 −2026 −378 175 −1776 955 −1737 −865 378 −1208 1258 −25 −120 −230 −1349 −1927 −1220 557 — * * * * * * * * * * * * * * * * * * * * — * * * * * * * * 0

TABLE 13 HMMER2.0 [2.3.2] NAME beta_exp_seqs LENG 238 ALPH Amino RF no CS no MAP yes COM hmmbuild beta_hmm2 beta_exp_seqs.a COM hmmcalibrate beta_hmm2 NSEQ 8 DATE Fri Mar 30 18:30:25 2007 CKSUM 9853 XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4 NULT −4 −8455 NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644 EVD −152.743744 0.132052 HMM A C D E F G H I K L M N P Q R S T V W Y m->m m->i m->d i->m i->i d->m d->d b->m m->e −561 * −1634 1(M) −117 −798 −402 82 −937 −1185 8 −544 307 −732 2122 −103 −1331 1400 −38 722 −88 −348 −1198 −701 3 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 −561 * 2(E) −1082 −2012 315 3129 −2414 −1266 −581 −2231 −449 −2306 −1728 −179 −1697 −299 −873 −951 −1139 −1916 −2295 −1840 4 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 3(I) −1084 −859 −2738 −2477 −657 −2548 −1955 3104 −2151 274 286 −2280 −2665 −2051 −2174 −1970 −1120 1153 −1775 −1342 5 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 4(D) −728 −2252 2483 757 −2533 −947 −190 −2414 −229 −2389 −1646 2098 −1401 156 −906 −498 −777 −1953 −2549 −1708 6 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 5(E) −1082 −2012 315 3129 −2414 −1266 −581 −2231 −449 −2306 −1728 −179 −1697 −299 −873 −951 −1139 −1916 −2295 −1840 7 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 6(T) −222 −1283 7 357 −1665 −1092 81 −1315 1142 −1403 −595 1311 −1290 468 179 −182 1459 −978 −1664 −1099 8 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 7(L) −1470 −1280 −2669 −2428 −178 −2553 −1713 430 −2000 2666 707 −2294 −2628 −1837 −1953 −2111 −1483 53 −1355 −983 9 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 8(L) −1470 −1280 −2669 −2428 −178 −2553 −1713 430 −2000 2666 707 −2294 −2628 −1837 −1953 −2111 −1483 53 −1355 −983 10 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 9(R) −1293 −1707 −1553 −1016 −2181 −1720 −534 −2070 488 −1979 −1441 −1002 −1962 −244 3506 −1337 −1257 −1841 −1832 −1633 11 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 10(N) −492 −1682 390 456 −1956 −1087 −26 −1802 395 −1805 −1034 2238 −1386 2034 28 −379 −488 −1428 −1929 −1277 12 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 11(I) −1084 −859 −2738 −2477 −657 −2548 −1955 3104 −2151 274 286 −2280 −2665 −2051 −2174 −1970 −1120 1153 −1775 −1342 13 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 12(I) −1084 −859 −2738 −2477 −657 −2548 −1955 3104 −2151 274 286 −2280 −2665 −2051 −2174 −1970 −1120 1153 −1775 −1342 14 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 13(K) −505 −1727 −252 1422 −2133 −1318 183 −1765 1640 −1672 −851 28 −1413 612 1584 −390 −399 −1407 −1768 −1274 15 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 14(D) −658 −2242 2252 1435 −2514 853 −109 −2321 −87 −2279 −1497 422 −1362 259 −753 −428 −678 −1868 −2487 −1660 16 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 15(V) 748 −413 −2029 −1720 −969 −1515 −1368 951 −1502 −332 −2 −1391 −1912 −1365 −1614 −776 −423 2546 −1740 −1342 17 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 16(L) −1296 −988 −3217 −2703 −76 −3057 −1956 1866 −2372 2163 1015 −2560 −2822 −1967 −2303 −2251 −1234 794 −1447 −1233 18 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 17(N) 1158 −703 −271 −42 −1756 −755 −333 −1434 −123 −1598 −780 1499 −1204 −10 −522 1429 −63 −929 −1892 −1374 19 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 18(E) −1082 −2012 315 3129 −2414 −1266 −581 −2231 −449 −2306 −1728 −179 −1697 −299 −873 −951 −1139 −1916 −2295 −1840 20 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −701 −1378 * * 19(M) −510 −499 −2163 −1628 −454 −1946 −1027 664 −1344 132 3301 −1444 −2063 −1141 −1422 −1064 853 1357 −1169 −825 21 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 20(Q) −595 −1941 −47 1215 −2308 −1349 8 −1994 1563 −1906 −1064 12 −1502 2357 337 −457 −516 −1599 −2025 −1446 22 — −149 −498 233 43 −381 399 106 −626 210 −466 −720 275 394 46 96 360 118 −369 −294 −249 — −587 −1605 −7537 −189 −3027 −701 −1378 * * 21(T) −109 −928 −430 −253 −2011 1064 −575 −1692 −379 −1854 −1040 1320 −1426 −258 −774 −200 2174 −1178 −2146 −1631 24 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 22(S) 546 −1052 −378 86 −1686 381 −156 −1358 153 −1477 −642 −105 −1332 1007 −293 1168 1023 −960 −1778 −1219 25 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 23(D) −695 −2280 2543 1073 −2545 −1168 −161 −2333 770 −2265 −1430 221 −1490 1100 −543 −490 −678 −1880 −2447 −1670 26 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 24(K) −362 −1292 −506 92 −1508 −1378 26 −46 1672 −1229 −447 −128 −1457 402 833 −358 1354 −842 −1539 −1035 27 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 25(P) 277 −1467 −164 225 −1828 −1260 −33 −1485 934 −1547 −732 −28 2065 1055 98 −326 −342 −1148 −1799 −1237 28 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 26(I) −636 −547 −2348 −1836 −669 −2184 −1282 2090 −1586 −84 272 −1663 1335 −1395 −1679 −1311 −627 1784 −1415 −1037 29 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 27(S) −24 −544 −1523 −1279 −1545 −999 −1116 −753 −1130 −1366 −748 −952 −1562 −1005 −1316 2493 −298 1210 −1962 −1530 30 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 28(F) −2311 −1922 −3157 −3219 4089 −2828 −763 −1194 −2995 −797 −853 −2459 −3032 −2434 −2736 −2546 −2370 −1425 −113 961 31 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 29(T) −205 −1415 −93 368 −1801 194 53 −1500 1106 −1542 −677 847 −1311 472 −33 450 1223 −1112 −1785 −1163 32 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −24 −6495 −7537 −894 −1115 −701 −1378 * * 30(M) 1277 −412 −1511 −962 −449 92 −534 78 −746 841 1781 −899 −1714 −574 −925 −643 −242 174 −924 −542 33 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 31(Q) −302 −1783 866 1044 −2094 −1206 84 −1848 842 −1794 −879 744 −16 1626 −45 369 −247 −1401 −1967 −1274 34 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 32(A) 1179 −775 528 −218 −885 −1427 −232 −328 −104 479 11 −384 −1539 −5 −477 −420 394 364 −1220 −755 35 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −199 −6672 −3068 −894 −1115 −701 −1378 * * 33(K) 737 −1279 −218 331 −1510 −1210 95 −1172 1077 −1272 −440 61 339 495 575 312 −129 −85 −1572 −988 36 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −675 −6497 −1463 −894 −1115 −990 −1010 * * 34(S) 1592 −166 −797 −644 −1616 −422 −681 −1189 −623 −1515 −745 −366 −1034 −483 −858 1842 163 −617 −1871 −1468 37 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −37 −5859 −6901 −894 −1115 −1577 −589 * * 35(A) 1794 −226 −1352 −1036 −937 −920 −827 406 −846 −478 −30 −769 −1396 −729 −1028 −171 −53 1373 −1511 −1108 38 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −37 −5859 −6901 −894 −1115 −1577 −589 * * 36(A) 1875 −154 −826 −672 −1598 −421 −693 −1162 −644 −1496 −730 −380 −1035 −502 −873 1543 167 −597 −1860 −1462 39 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −37 −5859 −6901 −894 −1115 −1577 −589 * * 37(S) 326 −378 −424 −280 −1605 −535 −464 −1316 −311 −1530 −754 −204 1087 −215 −611 1952 64 −770 −1804 −1337 40 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −37 −5859 −6901 −894 −1115 −1577 −589 * * 38(A) 1607 −764 −122 105 −1345 −864 −110 −927 240 −1134 −440 −28 −1219 1445 −70 −82 −102 −620 −1536 −1024 41 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −37 −5859 −6901 −894 −1115 −1577 −589 * * 39(A) 1803 −165 −972 −743 −1388 −531 −683 −645 −621 −1166 −492 −458 −1107 −509 −835 189 1642 −256 −1723 −1338 42 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −262 −5859 −2747 −894 −1115 −1577 −589 * * 40(T) 355 −282 −441 −217 −1238 −551 −308 −723 −120 −1049 −364 −157 1124 −76 −394 184 1668 −353 −1502 −1065 43 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −44 −5640 −6682 −894 −1115 −1694 −534 * * 41(V) 1381 −162 −1227 −907 −788 −898 −715 613 −712 −293 125 −683 −1345 −611 −902 −143 7 1512 −1390 −978 44 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −44 −5640 −6682 −894 −1115 −1694 −534 * * 42(T) 1348 −93 −776 −557 −1228 −464 −527 −425 −433 −977 −333 −323 −1020 −345 −654 258 1732 −77 −1578 −1179 45 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −44 −5640 −6682 −894 −1115 −1250 −787 * * 43(A) 1932 −257 −1412 −1102 −1001 −936 −884 327 −912 −552 −96 −813 −1424 −790 −1089 −191 −85 1321 −1569 −1170 46 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −35 −5944 −6986 −894 −1115 −1199 −825 * * 44(A) 2371 −274 −1256 −1069 −1658 −626 −968 −939 −950 −1454 −768 −679 −1243 −815 −1130 75 1222 −496 −1989 −1628 47 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 * * 45(P) −1069 −1335 −1496 −1611 −2330 −1424 −1603 −2332 −1655 −2427 −1993 −1508 3827 −1636 −1724 −1268 −1357 −1941 −2164 −2168 48 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 * * 46(V) −182 −442 −1500 −1190 −857 −1313 −928 462 −980 −418 3 −1007 702 −888 −1142 −563 −322 2411 −1454 −1039 49 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 * * 47(A) 1619 −670 −305 −86 −1730 −748 −364 −1400 −169 −1579 −768 1506 −1208 −53 −557 903 −62 −901 −1883 −1375 50 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 * * 48(G) −883 −1213 −1453 −1640 −2599 3389 −1702 −2667 −1883 −2795 −2238 −1465 −1887 −1747 −1938 −1085 −1224 −2070 −2309 −2422 51 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −302 −6123 −2519 −894 −1115 −1391 −693 * * 49(D) 1124 −1254 2055 577 −1863 −791 −92 −1454 1 −1671 −928 298 −1204 245 −533 −149 −294 −1070 −1981 −1326 52 — −149 −500 233 43 −381 399 106 −626 211 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −1023 −2195 −1788 −353 −2203 −1133 −879 * * 50(D) −523 −2067 2082 1864 −2301 −772 50 −2094 60 −2087 −1336 595 −1196 407 −594 −290 −546 −1670 −2291 −1479 54 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 51(G) −313 −704 −806 −934 −1944 3026 −1044 −1895 −1126 −2082 −1505 −815 −1368 −1020 −1245 −501 −624 −1373 −1779 −1747 55 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 52(P) 1565 −250 −551 −379 −1407 −503 −474 −908 −350 −1239 −536 −247 1592 −257 −615 205 126 −470 −1666 −1248 56 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 53(E) 1104 −1069 435 1528 −1553 −836 16 −1056 263 −1313 −590 250 −1178 352 −163 −109 −176 −750 −1687 −1104 57 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 54(P) −451 −806 −833 −877 −1665 −916 −922 −1540 −883 −1690 −1226 −823 3400 −878 −1016 −633 −706 −1199 −1631 −1492 58 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 55(E) −667 −1586 578 2707 −1926 −955 −206 −1660 −16 −1789 −1202 141 −1361 84 −417 −562 −713 −1376 −1884 −1396 59 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 56(K) −702 −1385 −456 −137 −1799 −1249 −32 −1476 2770 −1521 −915 −261 −1487 298 734 −689 −663 −1234 −1555 −1219 60 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 57(K) −242 −1146 −42 266 −1604 −1019 110 −1246 1179 −1332 −591 71 1256 469 482 −230 −239 −934 −1543 −1063 61 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1644 −556 * * 58(V) −692 −560 −2296 −1824 −148 −2252 −1309 1284 −1503 1481 845 −1707 −2262 −1329 −1585 −1428 −681 1916 −1248 −872 62 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −41 −5738 −6781 −894 −1115 −1056 −946 * * 59(D) −368 −1609 2275 599 −2245 503 −149 −2013 −61 −2043 −1235 290 −1304 215 −647 738 −448 −1529 −2272 −1549 63 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 * * 60(W) −1818 −1380 −2886 −2725 2827 −2739 204 −861 −2341 −518 −402 −1751 −2707 −1673 −2100 −1954 −1745 −988 4383 2002 64 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 *1 * 61(F) −1369 −1089 −2974 −2547 2668 −2815 −904 479 −2229 1773 892 −2159 −2660 −1714 −2105 −1996 −1299 90 −319 513 65 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −1391 −693 * * 62(Q) −318 −1420 −175 306 −1747 −1213 155 −1383 1463 −1416 −607 66 −1336 1585 492 −256 1223 −1062 −1632 −1103 66 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −31 −6123 −7165 −894 −1115 −393 −2068 * * 63(H) −965 −2315 304 2557 −2345 −1402 2832 −2376 −96 −2333 −1572 −69 −1734 −25 −542 −787 −951 −1991 −2354 −1617 67 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 64(V) −243 −624 −951 −402 −698 164 −280 507 700 −540 158 −512 −1577 −129 −534 129 −199 1501 −1065 −632 68 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 65(G) −1103 −2683 1617 1044 −3018 2466 −544 −2852 −622 −2806 −2065 78 −1745 −197 −1335 −856 −1156 −2377 −3010 −2151 69 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 66(I) 127 −601 −1729 700 −741 −2010 −897 1823 −1032 −346 165 −1212 −2069 −884 −1251 −1065 −513 1823 −1321 −915 70 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 67(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 71 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 68(K) −672 −1700 −767 −106 −2028 −1594 −55 −1633 2386 −139 −843 −300 −1654 352 1135 118 −565 −1339 −1812 −1353 72 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 69(P) 810 −1146 −439 −69 −1871 −1144 −337 −1545 −33 −1667 −841 −243 2101 1026 −457 727 −307 −1127 −1964 −1414 73 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 70(G) −1150 −2176 1681 −110 −3126 2890 −1035 −3045 −1194 −3069 −2369 −410 −1958 −753 −1812 −1057 −1331 −2472 −3057 −2480 74 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 71(E) −251 −1222 −293 1202 −1362 −1299 3 −1014 313 −180 −363 912 −1397 370 −141 −248 901 −750 −1509 844 75 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 72(Q) −574 −1746 −481 54 −2162 −1456 −57 −1827 650 −1790 −959 −177 −1574 2167 1643 1133 −504 −1461 −1934 −1408 76 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 73(Q) 181 −1571 −198 359 −1847 −1263 91 −1546 1095 −1559 −684 70 −1354 1506 888 829 −211 −322 −1779 −1157 77 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 74(D) −1284 −2925 3272 428 −3206 −1301 −666 −3170 −865 −3100 −2422 1520 −1826 −343 −1646 −999 −1367 −2663 −3228 −2302 78 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 75(E) −1877 −2708 −271 3539 −3235 −1868 −1322 −3210 −1299 −3203 −2676 −849 −2342 −1076 −1736 −1723 −1961 −2857 −2976 −2640 79 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 76(V) −1294 −1167 −3176 −3053 −1510 −2597 −2599 776 −2808 −665 −568 −2665 −2928 −2721 −2804 −2093 −1429 3343 −2527 −2099 80 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 77(V) −1387 −983 −3817 −3421 −1325 −3517 −3036 2126 −3234 −247 −157 −3173 −3403 −3060 −3284 −2792 −1380 2968 −2682 −2263 81 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 78(I) −1608 −1307 −3432 −3238 −1179 −3095 −2687 3474 −2941 −191 −206 −2987 −3216 −2818 −2915 −2635 −1653 832 −2363 −1952 82 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 79(G) 2003 −693 −1730 −1779 −2673 2541 −1744 −2343 −1885 −2638 −1819 −1201 −1639 −1627 −1995 −291 −469 −1512 −2872 −2608 83 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 80(V) −1377 −984 −3782 −3387 −1310 −3475 −2994 1952 −3192 −238 −150 −3138 −3378 −3020 −3242 −2751 −1373 3039 −2656 −2235 84 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 81(G) 2256 −685 −1743 −1778 −2653 2305 −1732 −2321 −1869 −2616 −1796 −1198 −1633 −1613 −1982 −284 −459 −1496 −2856 −2589 85 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 82(P) −2089 −2190 −2567 −2787 −3372 −2253 −2663 −3607 −2895 −3590 −3215 −2612 4122 −2846 −2839 −2316 −2418 −3135 −2975 −3234 86 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 83(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 87 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 84(F) −2605 −2164 −3410 −3518 4186 −3034 −940 −1504 −3300 −1072 −1142 −2698 −3246 −2697 −2999 −2815 −2670 −1743 −274 806 88 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 85(D) 1368 −1571 1781 109 −2469 1557 −540 −2201 −430 −2276 −1455 −143 −1580 −184 −971 −465 −630 −1675 −2520 −1865 89 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 86(K) −543 −1715 −308 1446 −1982 −1429 −43 −1616 1943 117 −835 −107 −1542 369 238 −460 −465 −1300 −1873 −1318 90 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 87(Y) 768 −433 −1427 −878 −230 −1635 1002 28 −656 −299 359 −841 −1719 −495 −842 −672 −251 1365 −705 2062 91 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 88(Q) −713 −1108 −1266 −711 −658 −1906 −585 −189 −306 733 1857 −852 −1945 2725 −557 −934 −646 −321 −1349 −939 92 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 89(H) −509 −1727 1105 324 −1970 −1263 2947 −1737 98 −1797 −978 19 −1505 215 −403 −416 1593 −1376 −2025 −1356 93 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 90(H) 257 −1526 −246 303 −1778 −1296 1995 −1458 1182 −1499 −646 24 −1387 1802 104 −236 −241 −136 −1741 −1141 94 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 91(N) −205 −1061 −522 −287 −2096 −1074 −595 −1786 −360 −1930 −1104 2136 −1508 −259 −766 834 2022 −1276 −2209 −1676 95 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 92(M) −1753 −1370 −3997 −3423 −278 −3613 −2472 2205 −3134 2048 2292 −3216 −3244 −2474 −2907 −2799 −1664 281 −1734 −1673 96 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 93(V) −961 −720 −3067 −2568 −1001 −2736 −1942 2099 −2323 −277 18 −2312 −2758 −2110 −2361 −1900 1460 2254 −1899 −1513 97 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 94(G) −798 −2252 1834 409 −2613 1891 −331 −2390 1009 −2358 −1542 66 −1618 55 −720 −619 −806 −1944 −2552 −1804 98 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 95(M) −1293 −943 −3609 −3096 −758 −3225 −2328 2635 −2838 287 2654 −2820 −3076 −2478 −2765 −2408 −1249 1673 −1950 −1676 99 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 96(P) −396 −1347 −270 −70 −2090 −1203 −421 −1805 −70 −1895 −1089 −261 2547 1365 −449 727 −492 −1369 −2141 −1577 100 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 97(H) −2323 −2419 −1868 −1956 −1368 −2396 5124 −3143 −1645 −2924 −2595 −1989 −2806 −1873 −1712 −2381 −2439 −2923 −1719 −935 101 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 98(K) −680 −2096 1229 360 −2455 −130 −204 −2203 2524 −2158 −1316 37 −1567 204 −321 −526 −656 −1772 −2326 −1640 102 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 99(K) 848 −1793 901 1050 −2097 −1210 62 −1847 1061 −1803 −896 831 −1350 508 −83 367 −273 −1407 −1983 −1291 103 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 100(I) −1608 −1307 −3432 −3238 −1179 −3095 −2687 3474 −2941 −191 −206 −2987 −3216 −2818 −2915 −2635 −1653 832 −2363 −1952 104 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 101(L) −1989 −1594 −4052 −3541 −273 −3812 −2656 1199 −3191 2712 863 −3408 −3403 −2575 −2992 −3067 −1907 143 −1822 −1721 105 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 102(R) −1611 −2371 −2012 −943 −3103 −2203 −236 −2516 2118 −2241 −1553 −907 −2186 179 3088 −1505 −1353 −2267 −2130 −1964 106 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 103(E) −933 −2372 367 2531 −2656 −1364 −342 −2437 −15 −2373 −1593 −9 −1695 2152 −461 −741 −915 −2027 −2525 −1824 107 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 104(M) −1412 −1074 −3701 −3133 −384 −3239 −2170 1563 −2844 1897 2189 −2846 −3015 −2314 −2666 −2399 −1341 1581 −1647 −1485 108 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 105(I) −1408 −993 −3860 −3463 −1315 −3565 −3081 2912 −3283 −218 −139 −3218 −3431 −3098 −3328 −2843 −1397 2338 −2695 −2286 109 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 106(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 110 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 107(G) −1814 −2025 −2478 −2748 −3577 3650 −2700 −3847 −3052 −3864 −3354 −2492 −2699 −2874 −2984 −2040 −2187 −3148 −3081 −3439 111 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 108(I) −1408 −993 −3860 −3463 −1315 −3565 −3081 2912 −3283 −218 −139 −3218 −3431 −3098 −3328 −2843 −1397 2338 −2695 −2286 112 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 109(E) −1877 −2708 −271 3539 −3235 −1868 −1322 −3210 −1299 −3203 −2676 −849 −2342 −1076 −1736 −1723 −1961 −2857 −2976 −2640 113 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 110(E) −1877 −2708 −271 3539 −3235 −1868 −1322 −3210 −1299 −3203 −2676 −849 −2342 −1076 −1736 −1723 −1961 −2857 −2976 −2640 114 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 111(E) −1877 −2708 −271 3539 −3235 −1868 −1322 −3210 −1299 −3203 −2676 −849 −2342 −1076 −1736 −1723 −1961 −2857 −2976 −2640 115 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 112(G) −1814 −2025 −2478 −2748 −3577 3650 −2700 −3847 −3052 −3864 −3354 −2492 −2699 −2874 −2984 −2040 −2187 −3148 −3081 −3439 116 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 113(L) −1899 −1496 −4056 −3537 −330 −3780 −2667 1776 −3219 2526 815 −3381 −3385 −2604 −3022 −3020 −1820 326 −1861 −1762 117 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 114(K) −1189 −2157 −978 −437 −2386 −1859 2668 −2247 2797 −2096 −1365 −571 −1954 163 690 −1086 −1045 −1950 −1998 −1554 118 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 115(A) 2868 −600 −1841 −1808 −2491 −872 −1666 −2124 −1780 −2447 −1635 −1169 −1579 −1551 −1883 1107 −372 −1350 −2748 −2442 119 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 116(R) −2176 −2415 −2504 −1923 −3054 −2403 −1269 −3053 −167 −2856 −2350 −1844 −2662 −997 3887 −2228 −2131 −2807 −2501 −2452 120 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 117(V) −1376 −984 −3781 −3386 −1309 −3474 −2993 1947 −3191 −238 −150 −3136 −3377 −3019 −3241 −2749 −1373 3041 −2655 −2234 121 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 118(V) −1387 −983 −3817 −3421 −1325 −3517 −3036 2126 −3234 −247 −157 −3173 −3403 −3060 −3284 −2792 −1380 2968 −2682 −2263 122 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 119(R) −1606 −2372 −2007 −936 −3104 −2201 −232 −2516 2216 −2240 −1550 −902 −2183 184 3024 −1499 −1347 −2267 −2130 −1962 123 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 120(C) −1091 2585 −3500 −3018 −1078 −3015 −2288 2503 −2770 −275 −25 −2674 −2983 −2523 −2739 −2210 −1074 2201 −2079 −1689 124 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 121(Y) −1005 −771 −3013 −2486 1856 −2498 −774 1186 −2131 1308 517 −1953 −2456 −1645 −1947 −1603 −935 12 −328 2462 125 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 122(R) −1618 −2368 −2015 −954 −3097 −2205 −244 −2514 1958 −2242 −1557 −915 −2192 170 3178 −1515 −1361 −2267 −2132 −1967 126 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 123(T) −31 −658 −1594 −1483 −2301 −927 −1425 −1916 −1414 −2229 −1444 −1045 −1586 −1265 −1583 1349 3012 −1257 −2555 −2195 127 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 124(S) −425 −1013 −1661 −1792 −2558 −1187 −1802 −2565 −1897 −2800 −2098 −1367 −1878 −1746 −1993 3270 −814 −1808 −2742 −2368 128 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 125(D) −2059 −2867 3860 −532 −3452 −1877 −1516 −3665 −1847 −3616 −3145 −882 −2414 −1318 −2469 −1872 −2198 −3236 −3167 −2847 129 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 126(V) −1294 −1167 −3176 −3053 −1510 −2597 −2599 776 −2808 −665 −568 −2665 −2928 −2721 −2804 −2093 −1429 3343 −2527 −2099 130 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 127(S) 1433 −625 −1729 −1673 −2531 −873 −1599 −2195 −1665 −2487 −1661 −1113 −1571 −1450 −1807 2786 −373 −1395 −2764 −2440 131 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 128(F) −2605 −2164 −3410 −3518 4186 −3034 −940 −1504 −3300 −1072 −1142 −2698 −3246 −2697 −2999 −2815 −2670 −1743 −274 806 132 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 129(M) −778 2068 −3064 −2496 −565 −2504 −1497 1629 −2179 11 2514 −2111 −2492 −1852 −2073 −1628 −733 2095 −1332 −989 133 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 130(A) 2576 −604 −1716 −1592 −2503 895 −1526 −2207 −1588 −2442 −1589 −1066 −1541 −1356 −1766 1089 −332 −1390 −2720 −2403 134 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 131(W) 1057 −423 −1396 −834 −306 −1603 1535 20 −598 −296 367 −808 −1690 −452 −791 −637 −225 1077 2854 −289 135 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 132(D) −1299 −3082 2892 2152 −3290 −1299 −612 −3186 −788 −3089 −2397 95 −1806 −275 −1580 −987 −1355 −2689 −3263 −2321 136 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 133(A) 1980 −568 −1754 −1474 −1797 1737 −1271 −1167 −1349 −1645 −923 −1058 −1593 −1159 −1530 −274 −322 876 −2145 −1790 137 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 134(D) 1587 −2023 1916 376 −2494 −1219 −341 −2252 −201 −2265 −1443 1603 −1579 40 −787 −539 −712 −1798 −2489 −1753 138 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 135(H) −380 −1637 −315 259 −1928 −1346 1589 −1609 1185 −1608 −749 1433 −1428 490 1250 −293 −304 142 −1806 −1220 139 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 136(L) −2228 −1908 −3555 −3373 −604 −3255 −2515 −62 −2963 2976 358 −3207 −3275 −2644 −2802 −2988 −2232 −567 −1904 −1643 140 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 137(S) −425 −1013 −1661 −1792 −2558 −1187 −1802 −2565 −1897 −2800 −2098 −1367 −1878 −1746 −1993 3270 −814 −1808 −2742 −2368 141 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 138(G) −1814 −2025 −2478 −2748 −3577 3650 −2700 −3847 −3052 −3864 −3354 −2492 −2699 −2874 −2984 −2040 −2187 −3148 −3081 −3439 142 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 139(S) −425 −1013 −1661 −1792 −2558 −1187 −1802 −2565 −1897 −2800 −2098 −1367 −1878 −1746 −1993 3270 −814 −1808 −2742 −2368 143 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 140(G) −1814 −2025 −2478 −2748 −3577 3650 −2700 −3847 −3052 −3864 −3354 −2492 −2699 −2874 −2984 −2040 −2187 −3148 −3081 −3439 144 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 141(I) −1608 −1307 −3432 −3238 −1179 −3095 −2687 3474 −2941 −191 −206 −2987 −3216 −2818 −2915 −2635 −1653 832 −2363 −1952 145 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 142(G) 1140 −633 −1604 −1487 −2567 2055 −1485 −2286 −1518 −2503 −1638 −1021 −1538 −1291 −1723 1944 −340 −1439 −2760 −2429 146 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 143(I) −1412 −1016 −3813 −3418 −1236 −3507 −3003 3157 −3215 −130 −75 −3172 −3393 −3017 −3250 −2786 −1406 1884 −2614 −2221 147 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 144(G) 1810 −701 −1719 −1781 −2689 2678 −1755 −2362 −1900 −2657 −1839 −1206 −1645 −1640 −2008 −299 −478 −1525 −2885 −2624 148 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 145(I) −1412 −1016 −3813 −3418 −1236 −3507 −3003 3157 −3215 −130 −75 −3172 −3393 −3017 −3250 −2786 −1406 1884 −2614 −2221 149 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 146(Q) −1762 −2287 −1132 −1144 −2520 −2040 −1289 −2773 −740 −2625 −2183 −1302 −2433 4141 −881 −1765 −1840 −2519 −2457 −2076 150 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 147(S) −425 −1013 −1661 −1792 −2558 −1187 −1802 −2565 −1897 −2800 −2098 −1367 −1878 −1746 −1993 3270 −814 −1808 −2742 −2368 151 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 148(K) −1892 −2397 −1701 −1300 −3050 −2216 −948 −2829 3583 −2696 −2116 −1348 −2450 −619 78 −1865 −1815 −2562 −2460 −2321 152 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 149(G) −1814 −2025 −2478 −2748 −3577 3650 −2700 −3847 −3052 −3864 −3354 −2492 −2699 −2874 −2984 −2040 −2187 −3148 −3081 −3439 153 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 150(T) −665 −1148 −2040 −2098 −2442 −1424 −1936 −1850 −1956 −2305 −1815 −1645 −2071 −1911 −2001 −909 3609 −1444 −2672 −2426 154 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 151(T) −665 −1148 −2040 −2098 −2442 −1424 −1936 −1850 −1956 −2305 −1815 −1645 −2071 −1911 −2001 −909 3609 −1444 −2672 −2426 155 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 152(V) −1396 −983 −3847 −3450 −1338 −3551 −3074 2356 −3271 −253 −163 −3204 −3424 −3095 −3321 −2828 −1387 2847 −2706 −2287 156 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 153(I) −1608 −1307 −3432 −3238 −1179 −3095 −2687 3474 −2941 −191 −206 −2987 −3216 −2818 −2915 −2635 −1653 832 −2363 −1952 157 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 154(H) −2323 −2419 −1868 −1956 −1368 −2396 5124 −3143 −1645 −2924 −2595 −1989 −2806 −1873 −1712 −2381 −2439 −2923 −1719 −935 158 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 155(Q) −1762 −2287 −1132 −1144 −2520 −2040 −1289 −2773 −740 −2625 −2183 −1302 −2433 4141 −881 −1765 −1840 −2519 −2457 −2076 159 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 156(K) −1383 −2308 −1643 −684 −2977 −2064 −157 −2423 2495 −2166 −1434 −724 −2050 1271 2417 −1265 −1155 −2139 −2093 −1856 160 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 157(D) −1253 −2521 3255 144 −3228 1167 −878 −3157 −1066 −3127 −2440 −203 −1918 −574 −1777 −1067 −1387 −2610 −3163 −2448 161 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 158(L) −1096 −1358 −1544 −1152 −644 −2175 −905 −263 −552 2080 225 −1234 −2259 2027 −699 −1367 −1047 −506 −1463 −1019 162 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 159(L) 519 −957 −510 761 −1084 −1377 −186 −643 18 943 −162 −266 741 91 −375 −396 −256 −453 −1365 −873 163 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 160(P) −2089 −2190 −2567 −2787 −3372 −2253 −2663 −3607 −2895 −3590 −3215 −2612 4122 −2846 −2839 −2316 −2418 −3135 −2975 −3234 164 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 161(L) −2228 −1908 −3555 −3373 −604 −3255 −2515 −62 −2963 2976 358 −3207 −3275 −2644 −2802 −2988 −2232 −567 −1904 −1643 165 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 162(S) −309 −1107 −487 −525 −2412 −1057 −961 −2282 −840 −2422 −1619 1332 −1628 −688 −1197 2732 −580 −1619 −2564 −2005 166 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 163(N) −1468 −2069 −762 −997 −2634 −1768 −1516 −3059 −1508 −3127 −2580 4001 −2312 −1391 −1789 −1503 −1700 −2572 −2658 −2191 167 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 164(L) −2228 −1908 −3555 −3373 −604 −3255 −2515 −62 −2963 2976 358 −3207 −3275 −2644 −2802 −2988 −2232 −567 −1904 −1643 168 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 165(E) −1877 −2708 −271 3539 −3235 −1868 −1322 −3210 −1299 −3203 −2676 −849 −2342 −1076 −1736 −1723 −1961 −2857 −2976 −2640 169 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 166(L) −2228 −1908 −3555 −3373 −604 −3255 −2515 −62 −2963 2976 358 −3207 −3275 −2644 −2802 −2988 −2232 −567 −1904 −1643 170 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 167(F) −2605 −2164 −3410 −3518 4186 −3034 −940 −1504 −3300 −1072 −1142 −2698 −3246 −2697 −2999 −2815 −2670 −1743 −274 806 171 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 168(P) −168 −833 −1242 −1249 −2494 −996 −1398 −2287 −1361 −2495 −1696 −974 3089 −1209 −1577 1589 −518 −1540 −2679 −2284 172 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 169(Q) −1762 −2287 −1132 −1144 −2520 −2040 −1289 −2773 −740 −2625 −2183 −1302 −2433 4141 −881 −1765 −1840 −2519 −2457 −2076 173 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 170(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 174 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 171(P) −2089 −2190 −2567 −2787 −3372 −2253 −2663 −3607 −2895 −3590 −3215 −2612 4122 −2846 −2839 −2316 −2418 −3135 −2975 −3234 175 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 172(L) −1534 −1220 −3652 −3169 −506 −3314 −2387 842 −2852 2266 595 −2942 −3144 −2433 −2752 −2537 −1497 1919 −1863 −1646 176 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 173(L) −2228 −1908 −3555 −3373 −604 −3255 −2515 −62 −2963 2976 358 −3207 −3275 −2644 −2802 −2988 −2232 −567 −1904 −1643 177 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 174(T) −687 −1734 1996 82 −2437 −1245 −620 −2077 −553 −2262 −1517 −192 −1688 −289 −1091 −639 2593 −1655 −2537 −1885 178 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 175(L) −865 −1070 −1980 −1784 −992 639 −1400 −475 −1550 2482 −226 −1598 −2221 −1461 −1629 −1221 −995 −514 −1675 −1284 179 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 176(D) −1277 −3051 2640 2426 −3262 −1297 −598 −3152 −756 −3057 −2356 97 −1797 −258 −1537 −970 −1330 −2657 −3231 −2297 180 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 177(T) 741 −572 −1785 −1337 −1017 −1495 −1012 1165 −1127 −673 −131 −1150 −1838 −980 −1309 −671 2444 264 −1540 −1155 181 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 178(Y) −2952 −2203 −3627 −3744 2312 −3534 −52 −1936 −3354 −1436 −1429 −2308 −3446 −2368 −2918 −2769 −2850 −2068 674 4232 182 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 179(R) −2176 −2415 −2504 −1923 −3054 −2403 −1269 −3053 −167 −2856 −2350 −1844 −2662 −997 3887 −2228 −2131 −2807 −2501 −2452 183 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 180(Q) 541 −1165 −629 −174 −1300 −1442 −271 −837 115 125 −362 −362 −1609 2716 −208 −502 −409 −670 −1574 −1069 184 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 181(I) −1608 −1307 −3432 −3238 −1179 −3095 −2687 3474 −2941 −191 −206 −2987 −3216 −2818 −2915 −2635 −1653 832 −2363 −1952 185 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 182(G) −1814 −2025 −2478 −2748 −3577 3650 −2700 −3847 −3052 −3864 −3354 −2492 −2699 −2874 −2984 −2040 −2187 −3148 −3081 −3439 186 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 183(K) −1892 −2397 −1701 −1300 −3050 −2216 −948 −2829 3583 −2696 −2116 −1348 −2450 −619 78 −1865 −1815 −2562 −2460 −2321 187 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 184(N) −1468 −2069 −762 −997 −2634 −1768 −1516 −3059 −1508 −3127 −2580 4001 −2312 −1391 −1789 −1503 −1700 −2572 −2658 −2191 188 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 185(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 189 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 186(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 190 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 187(R) −834 −2050 −746 884 −2527 −1656 −47 −2132 1493 −1981 −1160 −301 −1712 1458 2363 −712 −703 −1768 −2022 −1576 191 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 188(Y) −2742 −2290 −3014 −3139 701 −3008 −615 −2258 −2836 −1879 −1835 −2399 −3220 −2421 −2640 −2733 −2781 −2314 18 4561 192 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 189(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 193 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 190(K) −1556 −2356 −1907 −881 −3072 −2167 −223 −2498 2928 −2228 −1530 −865 −2155 194 2195 −1447 −1308 −2240 −2125 −1942 194 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 191(K) −871 −1938 −838 −231 −2459 1602 −133 −2052 1838 −1953 −1161 −399 −1768 286 1695 −786 −764 −1712 −2022 −1608 195 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 192(M) −677 −1520 −268 2336 −1519 −1510 −393 −1012 −85 −1090 2570 −321 −1724 −69 −467 −689 −653 −891 −1835 −1289 196 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 193(S) −425 −1013 −1661 −1792 −2558 −1187 −1802 −2565 −1897 −2800 −2098 −1367 −1878 −1746 −1993 3270 −814 −1808 −2742 −2368 197 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 194(P) −2089 −2190 −2567 −2787 −3372 −2253 −2663 −3607 −2895 −3590 −3215 −2612 4122 −2846 −2839 −2316 −2418 −3135 −2975 −3234 198 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 195(Q) −305 −1517 −118 288 −1938 −1216 −55 −1644 319 −1680 −817 1055 −1400 1561 −150 1420 724 −1244 −1916 −1290 199 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 196(P) −2089 −2190 −2567 −2787 −3372 −2253 −2663 −3607 −2895 −3590 −3215 −2612 4122 −2846 −2839 −2316 −2418 −3135 −2975 −3234 200 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 197(V) −1294 −1167 −3176 −3053 −1510 −2597 −2599 776 −2808 −665 −568 −2665 −2928 −2721 −2804 −2093 −1429 3343 −2527 −2099 201 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 198(P) −2089 −2190 −2567 −2787 −3372 −2253 −2663 −3607 −2895 −3590 −3215 −2612 4122 −2846 −2839 −2316 −2418 −3135 −2975 −3234 202 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 199(T) −410 −679 −2187 −1873 −1334 −1555 −1509 252 −1620 −819 −414 −1498 −2017 −1493 −1731 −819 2683 1729 −1987 −1601 203 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 200(V) −640 −504 −2456 −1869 −431 −2217 −1096 1416 −1525 927 467 −1651 −2226 −1310 527 −1301 −584 −2026 1123 −778 204 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — 21 −6672 −7714 −894 −1115 −701 −1378 * * 201(N) −1468 −2069 −762 −997 −2634 −1768 −1516 −3059 −1508 −3127 −2580 4001 −2312 −1391 −1789 −1503 −1700 −2572 −2658 −2191 205 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 202(D) −2059 −2867 3860 −532 −3452 −1877 −1516 −3665 −1847 −3616 −3145 −882 −2414 −1318 −2469 −1872 −2198 −3236 −3167 −2847 206 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 203(Q) −1762 −2287 −1132 −1144 −2520 −2040 −1289 −2773 −740 −2625 −2183 −1302 −2433 4141 −881 −1765 −1840 −2519 −2457 −2076 207 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 204(M) −1736 −1678 −2975 −2821 −903 −2619 −2137 −342 −2336 24 4777 −2581 −2886 −2281 −2280 −2241 −1843 −654 −1928 −1574 208 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 205(V) 2074 −651 −2390 −2110 −1415 −1553 −1686 337 −1896 −878 −474 −1620 −2049 −1709 −1970 −833 −621 2210 −2098 −1727 209 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 206(R) −2176 −2415 −2504 −1923 −3054 −2403 −1269 −3053 −167 −2856 −2350 −1844 −2662 −997 3887 −2228 −2131 −2807 −2501 −2452 210 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 207(P) −464 −829 −1870 −1666 −1484 −1471 −1433 −307 −1476 −1083 −664 −1375 2872 −1386 −1610 −788 −689 1790 −2036 −1633 211 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 208(K) −1185 −2213 −946 −394 −2739 −1847 −198 −2317 2731 −2136 −1393 −533 −1934 2194 759 −1067 −1032 −2005 −2126 −1773 212 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 209(Y) −2985 −2209 −3678 −3814 3177 −3561 −43 −1955 −3416 −1446 −1439 −2321 −3463 −2389 −2950 −2792 −2877 −2087 687 3771 213 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 210(Q) −762 −1364 −955 −522 −1120 −1735 −477 −761 27 −599 2479 −672 −1881 2972 −193 −868 −726 −767 −1578 −1080 214 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 211(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 215 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 212(K) −1047 −1653 −1327 −700 −1605 −1930 −401 −1092 2639 963 −645 −789 −2011 −70 384 −1110 −943 −1040 −1759 −1351 216 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 213(S) 2117 −614 −1759 −1672 −2492 −871 −1578 −2151 −1642 −2440 −1614 −1110 −1565 −1427 −1789 2362 −359 −1366 −2728 −2406 217 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 214(A) 3260 −995 −2154 −2261 −2604 −1267 −2060 −2035 −2233 −2502 −1934 −1631 −1961 −2058 −2240 −724 −871 −1499 −2817 −2619 218 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 215(L) −1899 −1496 −4056 −3537 −330 −3780 −2667 1776 −3219 2526 815 −3381 −3385 −2604 −3022 −3020 −1820 326 −1861 −1762 219 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 216(M) −1774 −1423 −3895 −3310 2035 −3493 −2118 380 −3002 2012 3024 −3054 −3139 −2298 −2747 −2656 −1671 −186 −1400 −1154 220 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 217(H) −2323 −2419 −1868 −1956 −1368 −2396 5124 −3143 −1645 −2924 −2595 −1989 −2806 −1873 −1712 −2381 −2439 −2923 −1719 −935 221 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 218(I) −1608 −1307 −3432 −3238 −1179 −3095 −2687 3474 −2941 −191 −206 −2987 −3216 −2818 −2915 −2635 −1653 832 −2363 −1952 222 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 219(K) −1892 −2397 −1701 −1300 −3050 −2216 −948 −2829 3583 −2696 −2116 −1348 −2450 −619 78 −1865 −1815 −2562 −2460 −2321 223 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 220(E) −1877 −2708 −271 3539 −3235 −1868 −1322 −3210 −1299 −3203 −2676 −849 −2342 −1076 −1736 −1723 −1961 −2857 −2976 −2640 224 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 221(T) −665 −1148 −2040 −2098 −2442 −1424 −1936 −1850 −1956 −2305 −1815 −1645 −2071 −1911 −2001 −909 3609 −1444 −2672 −2426 225 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 222(K) −1892 −2397 −1701 −1300 −3050 −2216 −948 −2829 3583 −2696 −2116 −1348 −2450 −619 78 −1865 −1815 −2562 −2460 −2321 226 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 223(H) −815 −1622 −721 −285 −154 −1730 3084 −1465 74 −1447 −801 −458 −1830 2002 −226 −776 −742 −1248 −656 1967 227 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 224(V) −1294 −1167 −3176 −3053 −1510 −2597 −2599 776 −2808 −665 −568 −2665 −2928 −2721 −2804 −2093 −1429 3343 −2527 −2099 228 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 225(V) −1294 −1167 −3176 −3053 −1510 −2597 −2599 776 −2808 −665 −568 −2665 −2928 −2721 −2804 −2093 −1429 3343 −2527 −2099 229 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 226(Q) 256 −702 −834 −278 −767 −1453 −225 −282 −122 −586 1554 −425 −1546 1737 −468 −439 956 664 −1117 −668 230 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 227(G) −983 −2484 2007 425 −2938 2044 −519 −2776 −557 −2732 −1965 1439 −1705 −165 −1244 −772 −1048 −2274 −2930 −2091 231 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 228(K) 1193 −1766 −773 −153 −2230 −1586 −104 −1829 2318 −1796 −998 −334 −1685 310 1077 −652 −627 −1498 −1931 −1482 232 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 229(K) 167 −1748 4 798 −2054 −1243 41 −1782 1364 −1757 −869 1291 1129 480 −27 −235 −297 −1367 −1949 −1287 233 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 230(P) 1754 −768 −1572 −1540 −2436 −1010 −1540 −2025 −1554 −2338 −1594 −1128 3036 −1391 −1716 −364 −511 −1375 −2661 −2342 234 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 231(Q) −302 −1026 −515 1250 −1130 −1413 −130 540 130 −886 −174 −226 −1505 1404 −275 −384 −243 962 −1392 −883 235 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 232(E) −520 −1863 141 2054 −2168 −1277 −101 −1881 266 −1884 −1038 55 −1487 1357 −204 −398 1225 −1490 −2100 −1438 236 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 233(L) −1842 −1440 −4032 −3516 −373 −3743 −2662 2090 −3208 2373 771 −3347 −3371 −2616 −3022 −2980 −1767 435 −1886 −1773 237 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 234(H) −414 −1810 −213 1423 −2160 −1330 1754 −1870 1213 −1798 −908 698 −1426 515 1275 −294 −339 −1451 −1937 −1321 238 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 235(V) −1402 −985 −3864 −3467 −1340 −3570 −3094 2599 −3291 −250 −162 −3221 −3436 −3114 −3341 −2848 −1392 2675 −2716 −2300 239 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 236(N) 920 −1434 421 730 −1653 −1247 54 147 406 −1410 −563 950 −1354 465 −80 −191 655 −999 −1694 −1080 240 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6672 −7714 −894 −1115 −701 −1378 * * 237(F) −544 −419 −2445 −1855 1863 −2055 −851 925 −1541 1449 674 −1547 −2075 −1212 −1484 287 −485 363 −744 −325 241 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −35 −5959 −7001 −894 −1115 −701 −1378 * * 238(N) 40 −510 −553 −161 −916 −1020 −206 −174 −45 −698 −25 1304 −1303 21 −374 −144 811 1214 −1256 −787 242 — * * * * * * * * * * * * * * * * * * * * — * * * * * * * * 0

TABLE 14 HMMER2.0 [2.3.2] NAME gamma_exp_seqs LENG 175 ALPH Amino RF no CS no MAP yes COM hmmbuild gamma_hmm3 gamma_exp_s

COM hmmcalibrate gamma_hmm3 NSEQ 8 DATE Fri Mar 30 18:50:16 2007 CKSUM 2849 XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4 NULT −4 −8455 NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644 EVD −145.815567 0.162883 HMM A C D E F G H I K L M N P Q R S T V W Y m->m m->i m->d i->m i->i d->m d->d b->m m->e −263 * −2585  1(M) −2974 −2816 −4159 −4200 −2091 −3504 −3380 −1690 −3763 −1179 5175 −3864 −3864 −3670 −3585 −3468 −3151 −2065 −3012 −2758 1 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 −263 *  2(S) −714 −1508 −1330 −1230 −3093 −1545 −1557 −2841 −1413 −2974 −2115 2328 −2119 −1253 −1778 2548 824 −2105 −3194 −2709 2 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  3(E) −1483 −3123 1915 2354 −3400 −1775 −923 −3218 −893 −3153 −2360 −393 −2183 −548 −1565 −1244 1945 −2744 −3349 −2503 3 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −1162 −7442 −869 −894 −1115 −701 −1378 * *  4(I) −1121 −753 −3426 −2999 −988 −3155 −2539 2556 −2786 50 147 −2768 −3061 −2590 −2831 −2397 −1109 2478 −2257 −1863 4 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −27 −6308 −7350 −894 −1115 −213 −2863 * *  5(D) −683 −2011 2419 66 −2272 −1583 −322 411 745 −2004 −1135 766 −1725 104 −470 −581 551 −1598 −2239 −1583 5 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  6(D) 887 −1768 1716 30 −2010 −1563 −265 −85 855 −1760 −908 −259 −1671 151 −387 −505 1220 −1340 −2037 −1419 6 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  7(M) −1176 −998 −3214 −2614 −619 −2754 −1611 1640 −2276 1581 2955 913 −2724 −1911 −2174 −1847 −1112 −23 −1437 −1150 7 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  8(E) −548 −1816 −459 1276 −2069 −1544 −222 −1765 164 −1806 −936 1067 −1642 207 567 394 406 1270 −2054 −1423 8 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  9(A) 2188 −871 −2463 −1968 −1209 −1970 −1409 1148 −1738 −981 −376 −1683 −2275 −1518 −1838 1104 −795 745 −1713 −1346 9 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −414 −7442 −2036 −894 −1115 −701 −1378 * *  10(M) −1361 −2209 −1685 −799 −2653 −2166 −374 −2159 2416 −2060 2788 −871 −2171 32 1952 −1303 −1181 −1918 −2154 −1850 10 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7045 −8087 −894 −1115 −1546 −605 * *  11(I) −1704 −1251 −4267 −3901 −1705 −3988 −3661 2754 −3767 −562 −486 −3661 −3816 −3619 −3839 −3300 −1696 2713 −3195 −2754 11 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −266 −7045 −2636 −894 −1115 −1546 −605 * *  12(R) 1194 −878 −1215 −668 −1215 −1507 −535 −506 −211 −969 −305 −716 −1730 −314 1866 −601 −426 1358 −1529 −1103 12 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  13(D) 1131 −2081 2040 353 −2427 −1304 −271 −2177 −37 −2169 −1326 21 −1589 2011 −588 −528 −665 −1749 −2383 −1663 13 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  14(Q) −741 −1209 −1130 −788 −1315 −1753 −757 −405 −373 −922 −481 −883 −1990 2875 −594 −942 −769 1796 −1762 −1264 14 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  15(L) −1993 −1572 −4217 −3701 −440 −3917 −2829 2053 −3397 2455 714 −3538 −3506 −2756 −3185 −3166 −1913 308 −1987 −1900 15 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * * 16(Q) −399 −1413 −310 −85 −2275 1203 −472 −2002 −154 −2063 −1220 −284 −1579 2043 −581 1618 −505 −1503 −2289 −1701 16 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −385 −6798 −2150 −894 −1115 −1832 −476 * *  17(R) −710 −1458 −960 −581 −2313 1738 −519 −2033 255 −2052 −1310 −650 −1763 −179 2634 −756 −786 −1624 −2089 −1747 17 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −25 −6438 −7480 −894 −1115 −2111 −380 * *  18(M) −369 −603 −1693 −1260 −653 −1556 −893 145 −936 −76 3276 −1117 −1837 −860 −1082 −738 1769 157 −1321 −946 18 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −25 −6438 −7480 −894 −1115 −1296 −754 * *  19(N) −811 −2335 341 1694 −2622 −1349 −285 −2396 −5 −2331 −1491 2186 −1641 2048 −522 −624 −785 −1955 −2502 −1765 19 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  20(S) 1743 −708 −1922 −1872 −2675 −960 −1760 −2350 −1853 −2638 −1802 −1250 −1669 −1622 −1975 2720 −467 −1516 −2907 −2602 20 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  21(A) 1407 −647 −1573 −1206 −1183 1260 −962 −633 −1050 1273 −348 −1021 −1738 −901 −1248 −528 −406 −420 −1612 −1224 21 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  22(Q) −943 −2552 1971 414 −2811 −1334 −397 −2628 −273 −2557 −1739 1988 −1696 2404 −888 −723 −941 −2164 −2737 −1929 22 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −247 −6798 −2753 −894 −1115 −1832 −476 * *  23(Q) 1213 −1833 1439 441 −2175 −1191 −112 −1906 160 −1918 −1072 120 −1444 2082 −368 −358 −468 −1496 −2144 −1452 23 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −23 −6573 −7616 −894 −1115 −1440 −663 * *  24(G) −78 −753 −1596 −1414 −2534 1905 −1434 −2239 −1404 −2450 −1598 −1042 −1626 −1216 −1645 1669 1888 −1468 −2725 −2364 24 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  25(T) −290 −1158 −455 992 −1440 −1324 −182 −1023 92 −1236 −454 −204 −1490 162 −346 1073 1304 618 −1621 −1082 25 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  26(A) 2848 −686 −2099 −2005 −2426 −1015 −1766 −1806 −1874 −2283 −1547 −1327 −1708 −1670 −1958 −344 1455 −1205 −2739 −2445 26 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  27(P) −117 −814 −1355 −1082 −2287 −1034 −1151 −1960 −1028 −2169 −1343 −882 2175 −896 −1323 1590 1811 −1330 −2476 −2063 27 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  28(A) 1889 −735 −1594 −1300 −2159 −1036 −1254 −1753 −1199 −2034 −1241 −997 1840 −1048 −1449 −299 1828 −1186 −2406 −2028 28 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  29(T) 1146 −812 −1308 −911 −2113 −1049 −968 −1778 −813 −1976 −1149 −782 1200 −689 −1149 1530 1750 −1213 −2300 −1865 29 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  30(A) 2697 −693 −1984 −1936 −2645 −959 −1785 −2307 −1901 −2607 −1776 −1275 −1671 −1663 −2006 1734 −463 −1490 −2890 −2593 30 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  31(T) 1184 −788 −1393 −1051 −2205 1459 −1096 −1875 −979 −2081 −1251 −859 1250 −835 −1291 −265 1793 −1268 −2400 −1985 31 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  32(A) 2154 −729 −1615 −1319 −2152 −1035 −1263 −1746 −1215 −2029 −1236 −1006 1292 −1061 −1461 −298 1830 −1180 −2403 −2027 32 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  33(T) 1265 −698 −1787 −1520 −2328 −990 −1421 −1963 −1411 −2231 −1411 −1092 −1625 −1232 −1627 1653 2477 −1298 −2566 −2217 33 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  34(Q) 1091 −1611 −110 225 −2092 488 −167 −1806 180 −1836 −972 1294 −1480 1910 −301 −326 −396 −1385 −2066 −1429 34 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  35(G) −578 −1644 1459 30 −2499 1928 −604 −2224 −483 −2306 −1490 −223 −1659 −249 −1016 −545 1609 −1718 −2557 −1909 35 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  36(Q) 807 −1248 −477 −5 −1851 −1220 −253 −1525 83 −1631 −790 −211 −1467 1893 −361 1196 1007 −1126 −1919 −1350 36 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  37(H) −351 −1466 −201 1372 −1675 −1321 2299 −1349 291 −1450 −624 −37 −1449 341 −175 687 −300 458 −1746 −1145 37 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  38(R) 1090 −1963 −1013 −316 −2459 −1757 −168 −2034 1908 −1949 −1158 −475 −1824 253 2199 −834 −795 −1708 −2033 −1626 38 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1832 −476 * *  39(Q) 992 −1745 −25 1456 −2070 −1277 −45 −1793 323 −1793 −913 46 −1436 1490 −175 727 −353 −1385 −2006 −1343 39 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −19 −6798 −7840 −894 −1115 −1118 −891 * *  40(L) 595 −1237 1114 −159 −1422 −1497 −383 −998 −194 1266 −487 −383 615 −84 −625 −554 −467 −781 −1681 −1163 40 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7045 −8087 −894 −1115 −1546 −605 * *  41(Y) −453 −1676 −399 153 −1937 616 −86 −1624 1122 −1661 −804 −125 −1534 338 985 905 −388 −1274 −1895 1329 41 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7045 −8087 −894 −1115 −1546 −605 * *  42(K) 858 −1517 −407 1108 −1738 −1452 −155 −1332 1346 −1489 −675 −178 −1563 238 −212 −417 −393 1080 −1811 −1232 42 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7045 −8087 −894 −1115 −348 −2223 * *  43(Q) 1257 −1903 −508 −123 −2463 −1568 −490 −2189 −125 −2201 −1319 1184 −1788 2352 −606 1081 −688 −1736 −2412 −1772 43 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  44(D) −3359 −3855 4091 −1760 −4660 −2944 −2744 −5137 −3226 −4926 −4562 −2110 −3493 −2619 −3804 −3177 −3522 −4662 −4114 −4101 44 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  45(Y) −4022 −3300 −4202 −4471 −162 −3952 −1522 −3554 −4217 −3007 −3045 −3501 −4179 −3602 −3853 −3928 −4069 −3631 −835 4786 45 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  46(P) −3569 −3390 −4042 −4362 −4763 −3426 −4034 −5349 −4542 −5126 −4838 −4133 4267 −4442 −4297 −3840 −3922 −4781 −4041 −4662 46 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  47(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 47 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  48(A) 2566 −1673 −3241 −2895 1995 −2977 −836 −1285 −2608 −1350 −940 −2278 −3082 −2148 −2520 −2124 −1792 −1243 −329 2352 48 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  49(T) 556 −1927 −417 1122 −2324 −1539 −326 −2051 46 −2052 −1161 914 −1696 109 −456 1237 1507 −1625 −2264 −1607 49 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  50(K) −1937 2062 −2451 −1377 −3415 −2629 −734 −2850 2929 −2646 −1935 −1362 −2625 −319 2284 −1860 −1722 −2588 −2595 −2389 50 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  51(H) −1373 3432 −2086 −1398 −1984 −2275 3997 −1962 −97 −2063 −1380 −1376 −2458 −680 1376 −1482 −1351 −1730 −2090 −1576 51 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  52(P) −877 −1501 −2346 −2559 −3585 −1679 −2607 −3503 −2784 −3706 −2880 −1979 3765 −2531 −2839 1007 −1303 −2500 −3737 −3472 52 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  53(E) −2083 −3956 1757 3314 −4156 −1930 −1331 −4122 −1636 −3984 −3363 −536 −2485 −1014 −2522 −1729 −2166 −3591 −4126 −3126 53 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  54(W) −918 −1893 −1053 −410 −2116 −1904 1403 −1750 939 191 −1025 −621 −1963 1572 1006 −890 −823 −1479 3807 −1561 54 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  55(I) −2035 −1558 −4660 −4320 −2086 −4410 −4230 3075 −4225 −895 −832 −4094 −4207 −4109 −4326 −3753 −2029 2515 −3685 −3220 55 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  56(K) −1221 −2380 −1220 −545 −2771 −2080 2222 −2412 2591 −506 −1493 −740 −2129 1828 236 −1118 −1094 −2072 −2372 −1913 56 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  57(T) −638 −1254 −2731 −2815 −3380 −1515 −2573 −3085 −2761 −3390 −2550 −1961 −2264 −2507 −2764 1207 3489 −2165 −3623 −3371 57 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  58(P) 1508 −1457 −2747 −2921 −3587 −1692 −2758 −3293 −2991 −3584 −2799 −2133 3639 −2719 −2975 −1098 −1293 −2385 −3752 −3588 58 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  59(T) −626 −1248 −2712 −2759 −3374 −1507 −2533 −3087 −2703 −3377 −2528 −1935 −2251 −2449 −2729 2022 3167 −2161 −3609 −3353 59 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  60(G) −1416 −2145 −1208 −1507 −3831 3184 −2206 −3881 −2367 −3956 −3204 2058 −2622 −2014 −2725 −1515 −1765 −2999 −3822 −3422 60 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  61(K) −3217 −3413 −3082 −2664 −4291 −3257 −2104 −4231 3883 −3955 −3433 −2635 −3520 −1813 −964 −3215 −3130 −3953 −3447 −3538 61 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  62(N) 997 −1468 −1225 −894 −2636 −1532 −1137 −2328 −901 −2462 −1613 2219 1916 −793 −1309 −715 1524 −1770 −2737 −2223 62 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  63(L) −2522 −2108 −4820 −4349 −929 −4400 −3462 148 −4047 2807 234 −4148 −4027 −3348 −3792 −3703 −2466 1342 −2528 −2466 63 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  64(T) −788 −2299 1480 1207 −2603 −1587 −371 −2366 1056 −2307 −1406 1363 −1766 69 −565 −641 1533 −1914 −2480 −1766 64 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  65(D) −2128 −4054 3436 1917 −4232 −1928 −1353 −4214 −1706 −4066 −3470 −529 −2498 −1042 −2637 −1757 −2219 −3673 −4218 −3179 65 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  66(I) −2331 −1899 −4695 −4181 1172 −4286 −3041 3269 −3918 981 175 −3898 −3911 −3237 −3671 −3510 −2255 6 −2249 −1928 66 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  67(T) −1680 −2119 −3327 −3541 −3740 −2348 −3223 −3396 −3447 −3750 −3249 −2849 −3039 −3332 −3347 −1934 3920 −2813 −3757 −3736 67 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  68(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 68 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  69(E) −2083 −3956 1757 3314 −4156 −1930 −1331 −4122 −1636 −3984 −3363 −536 −2485 −1014 −2522 −1729 −2166 −3591 −4126 −3126 69 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  70(N) 667 −2223 −552 −466 −2986 −1791 −914 −2689 705 −2706 −1900 3410 −2148 −538 −805 −1078 −1201 −2222 −2879 −2293 70 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  71(V) −2174 −1982 −4186 −4170 −2484 −3406 −3713 42 −3993 −1584 −1517 −3697 −3771 −3891 −3924 −3057 −2329 3646 −3507 −3148 71 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  72(L) −2729 −2229 −5089 −4576 −885 −4785 −3686 2209 −4314 2611 306 −4471 −4194 −3468 −3998 −4097 −2634 −271 −2572 −2589 72 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  73(N) 956 −2274 1213 −94 −2844 −1631 −685 −2609 −449 −2590 −1722 2259 −1939 −278 −1001 1526 −973 −2118 −2789 −2080 73 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  74(G) −1785 −3613 2207 1292 −3820 2213 −1112 −3710 −1261 −3595 −2872 1221 −2322 −764 −2053 −1476 −1833 −3198 −3789 −2827 74 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  75(K) −708 −2181 1118 746 −2508 −1590 −284 −2254 1976 −2187 −1279 1149 −1720 166 1307 −575 −648 −1808 −2344 −1667 75 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  76(V) −2021 −1555 −4630 −4292 −2072 −4355 −4168 1922 −4183 −894 −829 −4054 −4177 −4066 −4278 −3696 −2019 3287 −3647 −3180 76 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  77(K) −812 −2228 1238 34 −2594 1219 −435 −2340 1781 −2306 −1421 −281 −1809 −4 −596 −685 1266 −1898 −2493 −1804 77 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  78(A) 2961 −1382 −2807 −2930 −3515 −1634 −2710 −3207 −2947 −3502 −2695 −2093 2243 −2664 −2935 −1021 −1211 −2301 −3704 −3525 78 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  79(Q) −967 −2422 −345 1056 −2767 −1728 −447 −2497 1288 −2409 −1534 1390 −1900 2852 −350 −818 −905 −2063 −2544 −1892 79 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  80(D) −3359 −3855 4091 −1760 −4660 −2944 −2744 −5137 −3226 −4926 −4562 −2110 −3493 −2619 −3804 −3177 −3522 −4662 −4114 −4101 80 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  81(M) −2091 −1713 −4451 −3905 −954 −3989 −2968 336 −3615 1206 3494 −3630 −3713 −3048 −3417 −3183 −2024 2361 −2329 −2191 81 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  82(R) −3572 −3491 −3930 −3374 −4367 −3463 −2544 −4583 −1435 −4243 −3795 −3226 −3764 −2331 4134 −3660 −3534 −4308 −3567 −3769 82 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  83(I) −2506 −2132 −4383 −4301 −2107 −3870 −3753 3806 −4080 −1081 −1125 −4012 −4038 −3936 −3993 −3643 −2570 78 −3292 −2940 83 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  84(T) −624 −1248 −2705 −2744 −3377 −1505 −2523 −3093 −2688 −3379 −2528 −1928 −2247 −2435 −2721 2328 2957 −2163 −3609 −3352 84 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  85(P) −1686 −2611 −1672 −1050 −3249 −2373 −759 −2812 341 −2647 −1909 −1144 2832 1103 2390 −1606 −1559 −2494 −2639 −2322 85 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  86(D) 737 −2727 2071 1949 −3005 −1688 −649 −2797 −446 −2728 −1869 −302 −1983 1707 −1039 −948 −1120 −2333 −2908 −2133 86 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  87(T) −1680 −2119 −3327 −3541 −3740 −2348 −3223 −3396 −3447 −3750 −3249 −2849 −3039 −3332 −3347 −1934 3920 −2813 −3757 −3736 87 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  88(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 88 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  89(R) −1604 −2727 −1441 2175 −3300 −2312 −612 −2841 1592 −2626 −1849 −957 −2366 −182 2492 −1470 −1439 −2503 −2603 −2246 89 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  90(M) −2061 −1752 −4123 −3574 −361 −3659 −2041 −262 −3197 2031 3098 −3161 −3441 −2603 −2964 −2802 −1970 −720 −1446 2614 90 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  91(Q) −3221 −3397 −2531 −2668 −3852 −3172 −2736 −4443 −2278 −4160 −3817 −2785 −3623 4473 −2343 −3268 −3360 −4139 −3574 −3442 91 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  92(A) 2875 −1335 −2928 −3126 −3684 2067 −2851 −3441 −3216 −3720 −2847 −2129 −2353 −2834 −3138 −970 −1176 −2380 −3856 −3729 92 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  93(Q) 333 −2205 −255 1818 −2553 −1603 −404 −2298 −46 −2265 −1377 −266 −1788 2587 −554 426 −748 −1861 −2452 −1766 93 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  94(I) −2036 −1562 −4657 −4316 −2070 −4401 −4214 3210 −4217 −878 −820 −4089 −4201 −4095 −4314 −3744 −2031 2315 −3666 −3206 94 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  95(A) 3527 −1872 −3386 −3637 −3817 −2114 −3249 −3478 −3634 −3846 −3228 −2714 −2847 −3362 −3481 −1630 −1813 −2725 −3849 −3859 95 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  96(E) 657 −2144 −303 1992 −2460 −1564 −271 −2208 916 −2153 −1243 1296 −1696 1197 −360 −543 −616 −1765 −2321 −1636 96 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  97(D) −1428 −3090 2456 0 −3358 626 −868 −3185 −800 −3101 −2290 1589 −2147 2323 −1457 −1192 −1436 −2703 −3285 −2444 97 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  98(M) 2458 −1063 −2439 −2109 −2009 −1619 −1748 −1532 −1906 −1860 3077 −1662 −2171 −1718 −2036 877 −861 −1187 −2405 −2053 98 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * *  99(G) −1362 −2477 −504 −475 −2812 2719 1818 −2783 −476 −2759 −1994 −738 −2262 1773 −798 −1267 −1389 −2373 −2786 −2136 99 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 100(R) −3572 −3491 −3930 −3374 −4367 −3463 −2544 −4583 −1435 −4243 −3795 −3226 −3764 −2331 4134 −3660 −3534 −4308 −3567 −3769 100 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 101(H) −810 2146 2140 −126 −2275 −1646 2820 −2018 −274 −2093 −1264 −413 982 −165 −770 −747 −802 −1656 −2340 −1698 101 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 102(A) 2955 −1710 −2315 −1954 −3110 −1911 −1672 −2739 −942 −2886 −2159 −1698 −2440 −1397 1826 −1250 −1338 −2189 −3054 −2761 102 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 103(I) −2053 −1588 −4631 −4216 −1683 −4330 −3782 2657 −4066 1111 −476 −3979 −4072 −3758 −4047 −3615 −2023 2344 −3177 −2869 103 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 104(A) 2685 −1741 −1377 −1306 −2967 −1773 −1543 −2675 −1182 −2815 −2087 −1302 −2305 2631 1425 −1113 −1241 −2132 −3062 −2602 104 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 105(R) −2019 −2443 −2872 −1909 −2475 −2767 −1140 −2083 30 −1845 2733 −1796 −2859 −814 3466 −2113 −1903 −2079 −2488 −2193 105 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 106(N) −2749 −3127 −2054 −2376 −3897 −2856 −2871 −4637 −3059 −4563 −4104 4298 −3433 −2867 −3263 −2819 −3038 −4043 −3716 −3493 106 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 107(F) −3172 −2615 −4595 −4495 4044 −4307 −1507 −1008 −4122 717 −475 −3498 −4094 −3206 −3712 −3641 −3094 −1537 −738 314 107 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 108(R) −1406 −2631 −1037 1390 −3118 −2127 −576 −2728 338 −2561 −1757 −770 −2229 2482 2529 −1268 −1281 −2363 −2589 −2141 108 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 109(R) −3572 −3491 −3930 −3374 −4367 −3463 −2544 −4583 −1435 −4243 −3795 −3226 −3764 −2331 4134 −3660 −3534 −4308 −3567 −3769 109 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 110(A) 3527 −1872 −3386 −3637 −3817 −2114 −3249 −3478 −3634 −3846 −3228 −2714 −2847 −3362 −3481 −1630 −1813 −2725 −3849 −3859 110 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 111(A) 2600 −1202 −2867 −2874 −3511 640 −2600 −3272 −2839 −3509 −2602 −1948 −2213 −2506 −2861 2031 −1000 −2218 −3720 −3512 111 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 112(E) −3263 −3764 −1446 3823 −4534 −2947 −2635 −4813 −2809 −4652 −4247 −2114 −3462 −2477 −3224 −3106 −3391 −4409 −4008 −3980 112 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 113(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 113 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 114(T) −1377 −1356 −3473 −3179 −1732 −2771 −2620 2590 −2866 −935 −728 −2732 −3097 −2717 −2873 −2060 2702 379 −2680 −2298 114 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 115(A) 2529 −1216 −2947 −3039 −3523 −1479 −2704 −3262 −2994 −3544 −2659 −2017 −2245 −2654 −2953 2488 −1036 −2225 −3753 −3552 115 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 116(I) −2035 −1558 −4660 −4320 −2086 −4410 −4230 3075 −4225 −895 −832 −4094 −4207 −4109 −4326 −3753 −2029 2515 −3685 −3220 116 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 117(P) −877 −1501 −2346 −2559 −3585 −1679 −2607 −3503 −2784 −3706 −2880 −1979 3765 −2531 −2839 1007 −1303 −2500 −3737 −3472 117 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 118(D) −3359 −3855 4091 −1760 −4660 −2944 −2744 −5137 −3226 −4926 −4562 −2110 −3493 −2619 −3804 −3177 −3522 −4662 −4114 −4101 118 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 119(D) 1000 −3403 2984 1734 −3674 −1814 −1070 −3527 −1163 −3445 −2700 −428 −2286 −716 −1910 −1407 −1728 −3031 −3645 −2731 119 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 120(R) −1763 −2178 −2815 −2009 −2626 −2577 −1312 −1725 −215 −2141 −1691 −1848 −2804 −1008 3553 −1932 −1770 1073 −2627 −2315 120 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 121(I) −2084 −1616 −4657 −4243 −1653 −4365 −3815 3132 −4093 890 −443 −4016 −4093 −3767 −4069 −3656 −2054 1752 −3174 −2884 121 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 122(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 122 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 123(E) 100 −3437 1680 3073 −3701 −1819 −1079 −3559 −1178 −3473 −2732 −430 −2294 −725 −1928 −1421 −1748 −3060 −3669 −2750 123 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 124(I) −2462 −2009 −4861 −4316 −906 −4477 −3404 3063 −4059 1320 2522 −4133 −4007 −3310 −3785 −3718 −2374 −100 −2484 −2465 124 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 125(Y) −4022 −3300 −4202 −4471 −162 −3952 −1522 −3554 −4217 −3007 −3045 −3501 −4179 −3602 −3853 −3928 −4069 −3631 −835 4786 125 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 126(N) −2749 −3127 −2054 −2376 −3897 −2856 −2871 −4637 −3059 −4563 −4104 4298 −3433 −2867 −3263 −2819 −3038 −4043 −3716 −3493 126 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 127(A) 3159 −1216 −2991 −3125 −3530 −1484 −2756 −3260 −3083 −3557 −2679 −2048 −2256 −2728 −3008 1226 −1046 −2226 −3770 −3578 127 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 128(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 128 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 129(R) −3572 −3491 −3930 −3374 −4367 −3463 −2544 −4583 −1435 −4243 −3795 −3226 −3764 −2331 4134 −3660 −3534 −4308 −3567 −3769 129 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 130(P) −3569 −3390 −4042 −4362 −4763 −3426 −4034 −5349 −4542 −5126 −4838 −4133 4267 −4442 −4297 −3840 −3922 −4781 −4041 −4662 130 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 131(Y) −4048 −3064 −4528 −4828 3028 −4400 −683 −2944 −4397 −2302 −2372 −3069 −4275 −3191 −3805 −3657 −3917 −3103 65 4127 131 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 132(R) −3572 −3491 −3930 −3374 −4367 −3463 −2544 −4583 −1435 −4243 −3795 −3226 −3764 −2331 4134 −3660 −3534 −4308 −3567 −3769 132 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 133(S) −1298 −1844 −2875 −3136 −3678 −2026 −2995 −3857 −3302 −4033 −3306 −2458 −2758 −3055 −3253 3563 −1733 −2892 −3744 −3541 133 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 134(T) −628 −1249 −2717 −2772 −3374 −1509 −2541 −3085 −2715 −3378 −2532 −1940 −2253 −2461 −2737 1826 3269 −2161 −3611 −3356 134 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 135(K) −781 −1702 −906 −326 356 −1807 −409 −1467 2476 −1586 −831 −553 −1886 1683 −224 −780 −706 240 −1907 −1366 135 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 136(A) 1793 −2317 −182 1436 −2630 −1625 −446 −2381 −113 −2343 −1462 1229 −1828 1588 −633 −720 −823 −1946 −2530 −1828 136 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 137(E) −3263 −3764 −1446 3823 −4534 −2947 −2635 −4813 −2809 −4652 −4247 −2114 −3462 −2477 −3224 −3106 −3391 −4409 −4008 −3980 137 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 138(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 138 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 139(L) −2369 −2273 −3168 −2905 −984 −3520 −2341 −606 −2150 2925 −5 −2869 −3499 773 −2144 −2905 −2325 −1156 −2222 −1911 139 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 140(A) 3050 −2169 1557 −918 −3547 −1815 −1738 −3268 −1907 −3465 −2733 −1101 −2422 −1474 −2446 −1331 −1572 −2621 −3648 −3069 140 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 141(I) −1583 −1435 −3802 −3522 −1810 −3145 −2999 3393 −3226 −885 −741 −3089 −3390 −3083 −3224 −2453 1116 598 −2920 −2528 141 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 142(A) 3159 −1216 −2991 −3125 −3530 −1484 −2756 −3260 −3083 −3557 −2679 −2048 −2256 −2728 −3008 1226 −1046 −2226 −3770 −3578 142 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 143(D) 785 −2776 3038 −83 −3174 −1756 −838 −2947 1206 −2909 −2094 −427 −2118 −452 −1226 −1122 −1320 −2485 −3100 −2339 143 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 144(E) −2080 −3966 2020 3228 −4159 −1924 −1325 −4125 −1633 −3985 −3364 −528 −2480 −1008 −2526 −1723 −2163 −3592 −4133 −3124 144 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 145(L) −3371 −2888 −4716 −4628 −1407 −4203 −3653 −947 −4306 3212 −381 −4472 −4191 −3767 −3993 −4264 −3364 −1600 −2791 −2682 145 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 146(E) −1474 −2672 −1152 2693 −3190 −2187 −590 −2777 1538 −2592 −1796 −829 −2275 −160 1592 −1335 −1336 −2418 −2601 −2183 146 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 147(H) −854 −2355 1981 1100 −2650 −1609 2785 −2413 −120 −2363 −1474 −237 −1812 2 −665 423 558 −1968 −2546 −1827 147 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 148(K) −1846 −2756 −2163 −1201 −3441 −2548 −678 −2892 2670 −2658 −1923 −1238 −2547 −254 2397 −1742 1144 −2602 −2599 −2357 148 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 149(Y) −4032 −3074 −4494 −4762 743 −4354 −719 −3014 −4275 −2384 −2440 −3079 −4257 −3186 −3737 −3658 −3908 −3152 3572 4429 149 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 150(H) −1438 −2996 1391 −75 −3124 −1810 3844 −3065 −667 −2987 −2193 −429 −2172 2489 −1215 −1218 −1432 −2622 −3084 −2281 150 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 151(A) 3527 −1872 −3386 −3637 −3817 −2114 −3249 −3478 −3634 −3846 −3228 −2714 −2847 −3362 −3481 −1630 −1813 −2725 −3849 −3859 151 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 152(P) −1025 −2197 −1034 −410 −2631 −1925 −444 −2266 1763 −2207 −1375 −627 1881 −14 1096 −940 1601 −1905 −2333 −1837 152 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 153(V) −2029 −1557 −4631 −4241 −1861 −4362 −3936 2290 −4115 428 −638 −4011 −4123 −3892 −4149 −3664 −2008 2918 −3374 −2999 153 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 154(C) −1181 4220 −2589 −2685 −3120 −1960 −2546 −3057 −2657 −3393 −2695 3434 −2639 −2541 −2713 −1406 −1561 −2401 −3333 −2954 154 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 155(A) 3527 −1872 −3386 −3637 −3817 −2114 −3249 −3478 −3634 −3846 −3228 −2714 −2847 −3362 −3481 −1630 −1813 −2725 −3849 −3859 155 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 156(G) 2090 −1967 1316 −759 −3390 2191 −1492 −3161 −1542 −3255 −2440 −931 −2229 −1185 −2076 −1075 −1294 −2455 −3474 −2861 156 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 157(W) −3904 −3011 −4520 −4764 3780 −4316 −778 −2763 −4325 −2135 −2197 −3117 −4228 −3209 −3773 −3640 −3795 −2959 4425 1145 157 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 158(F) −1811 −1421 −4284 −3810 2418 −3829 −2928 2106 −3571 −448 −351 −3460 −3684 −3221 −3480 −3037 −1775 2416 −2494 −2056 158 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 159(R) 735 −2333 −863 1128 −2835 −1951 −604 −2472 145 −2416 −1604 −687 −2116 −188 2967 −1082 −1113 −2097 −2537 −2033 159 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 160(E) −3263 −3764 −1446 3823 −4534 −2947 −2635 −4813 −2809 −4652 −4247 −2114 −3462 −2477 −3224 −3106 −3391 −4409 −4008 −3980 160 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 161(A) 3142 −1216 −2990 −3120 −3529 −1483 −2753 −3260 −3078 −3556 −2677 −2046 −2255 −2723 −3005 1291 −1045 −2226 −3768 −3576 161 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 162(A) 3527 −1872 −3386 −3637 −3817 −2114 −3249 −3478 −3634 −3846 −3228 −2714 −2847 −3362 −3481 −1630 −1813 −2725 −3849 −3859 162 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 163(E) −1353 −2969 1558 2718 −3260 316 −827 −3072 −733 −3001 −2179 −361 −2110 −438 −1376 −1133 601 −2596 −3191 −2371 163 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 164(V) −770 −686 −2514 −1945 −447 −2242 −1009 −25 −1638 907 143 1079 −2296 −1373 −1632 −1314 −714 2075 −994 1702 164 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 165(Y) −4022 −3300 −4202 −4471 −162 −3952 −1522 −3554 −4217 −3007 −3045 −3501 −4179 −3602 −3853 −3928 −4069 −3631 −835 4786 165 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 166(E) −538 −1578 −594 1619 −1749 −1591 −271 182 64 −466 −709 −313 −1679 970 667 560 −477 169 −1859 −1283 166 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 167(Q) −583 −1987 −408 1304 −2289 −1544 −207 −2017 1086 −1990 −1087 −190 −1644 2012 −258 724 −522 445 −2178 −1519 167 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 168(R) −2066 −2822 −1934 −1456 −3549 −2571 −1068 −3277 108 −3049 −2371 1548 −2762 −689 3583 −2003 −1971 −2938 −2872 −2605 168 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 169(K) −963 −2461 926 23 −2770 −1679 1437 −2530 2489 −2458 −1580 1750 −1887 −51 −577 −804 −916 −2086 −2613 −1914 169 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 170(K) −3217 −3413 −3082 −2664 −4291 −3257 −2104 −4231 3883 −3955 −3433 −2635 −3520 −1813 −964 −3215 −3130 −3953 −3447 −3538 170 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 171(L) −2562 −2146 −4846 −4375 −908 −4427 −3477 110 −4068 2853 256 −4182 −4041 −3350 −3803 −3735 −2504 1163 −2519 −2467 171 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 172(K) −2474 −3104 −3210 −1788 −4002 −2957 −830 −3301 2947 −2931 −2275 −1649 −2892 −412 2812 −2351 −2137 −3082 −2748 −2679 172 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 173(G) −1037 −1749 −1285 −849 −2174 2283 −841 794 1846 −1893 −1188 −977 −2185 −518 −441 −1114 −1045 −1375 −2263 −1816 173 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 174(D) −1939 −3820 3052 1863 −4026 1028 −1223 −3952 −1477 −3823 −3155 −460 −2399 −893 −2340 −1601 −2010 −3421 −4014 −3000 174 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −12 −7442 −8484 −894 −1115 −701 −1378 * * 175(N) 355 −2563 2286 −73 −3075 −1686 −794 −2864 −638 −2825 −1980 2330 −2039 −399 −1233 944 −1176 −2366 −3020 −2262 175 — * * * * * * * * * * * * * * * * * * * * — * * * * * * * * 0

indicates data missing or illegible when filed 

1. A recombinant microbial host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion of: 1) 2,3-butanediol to 2-butanone; wherein the at least one DNA molecule is heterologous to said microbial host cell; and wherein said microbial host cell produces 2-butanol; and wherein the polypeptide that catalyzes a substrate to product conversion of 2,3-butanediol to 2-butanone is diol dehydratase or glycerol dehydratase, wherein the diol dehydratase or glycerol dehydratase comprise full length large, medium and small subunits that each give an E-value parameter of 0.01 or less when queried using a Profile Hidden Markov Model prepared using the large subunits of SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164; the medium subunits of SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; and the small subunits of SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; each query being carried out using the hmmsearch algorithm wherein the Z parameter is set to 1 billion.
 2. A recombinant microbial host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion of: i) 2,3-butanediol to 2-butanone; wherein the at least one DNA molecule is heterologous to said microbial host cell; and wherein said microbial host cell produces 2-butanone; and wherein the polypeptide that catalyzes a substrate to product conversion of 2,3-butanediol to 2-butanone is diol dehydratase or glycerol dehydratase, wherein the diol dehydratase or glycerol dehydratase comprise full length large, medium and small subunits that each give an E-value parameter of 0.01 or less when queried using a Profile Hidden Markov Model prepared using the large subunits of SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146 and 164; the medium subunits of SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; and the small subunits of SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; each query being carried out using the hmmsearch algorithm wherein the Z parameter is set to 1 billion. 3-7. (canceled)
 8. A host cell according to claims 1 or 2 wherein the cell is selected from the group consisting of: a bacterium, a cyanobacterium, a filamentous fungus and a yeast.
 9. A host cell according to claim 8 wherein the cell is a member of a genus selected from the group consisting of Clostridium, Zymomonas, Escherichia, Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Pediococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Pichia, Candida, Hansenula and Saccharomyces. 10-12. (canceled)
 13. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or glycerol dehydratase comprise full length large, medium and small subunits that each give an E-value parameter of 0.01 or less when queried using a Profile Hidden Markov Model prepared using the large subunits of SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164; the medium subunits of SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; and the small subunits of SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; each query being carried out using the hmmsearch algorithm wherein the Z parameter is set to 1 billion.
 14. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or the glycerol dehydratase identified by a process comprising the steps of: a) generating a Profile Hidden Markov Model from the alignment of the amino acid sequences corresponding to the large, medium and small subunits of diol and glycerol dehydratase enzymes wherein; i) the large subunit comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 99, 105, 135, 138, 141, 146, and 164; ii) the medium subunit comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 10, 101, 107, 136, 139, 142, 148, and 165; and iii) the small subunit comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:12, 103, 109, 137, 140, 143, 150, and 166; b) querying at least one public database of protein sequences containing sequences of diol and glycerol dehydratases with the Profile Hidden Markov Model of (a) using the hmmsearch algorithm wherein the Z parameter is set to 1 billion and the E-value parameter is set to 0.01, to identify a first data set of diol and glycerol dehydratase amino acid sequences; and c) removing any partial sequences from the first data set of (b) to generate a second data set of diol and glycerol dehydratase amino acid sequences, wherein diol dehydratase and the glycerol dehydratase enzymes are identified.
 15. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or glycerol dehydratase comprises a large subunit comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 93, 99, 105, 135, 138, 141, 146, 164, 167, 170, 173, 176, 179, 182, 185, 188, 191, 194, 197, 200, 203, 206, 209, 212, 215, 218, 221, 224, 227, 130, 243, 254, 255, 256, 257, 258 and 259, based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.
 16. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or glycerol dehydratase comprises a medium subunit comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 10, 95, 101, 107, 136, 139, 142, 148, 165, 168, 171, 174, 177, 180, 183, 186, 189, 192, 195, 198, 201, 204, 207, 210, 213, 216, 219, 222, 225, 228, 231, 244, 250, 252, 260, 261, 262, 263, 364, 265, 266, and 167 based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.
 17. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or glycerol dehydratase comprises a small subunit comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 12, 97, 103, 109, 137, 140, 143, 150, 166, 169, 172, 175, 178, 181, 184, 187, 190, 193, 196, 199, 202, 205, 208, 211, 214, 217, 220, 223, 226, 229, 232, 234, 236, 238, 240, 242, 245, 248, 249, 251, 253, 268, 270, 271, 272, 273, and 274, based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.
 18. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or glycerol dehydratase comprises fused large, medium and small subunits comprising an amino acid sequence having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 233, 235, 237, 239, 241, 246, and 247, based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.
 19. A host cell according to either of claims 1 or 2 wherein the diol dehydratase or glycerol dehydratase comprises fused large, medium and small subunits and has at least 95% identity to an amino acid sequence comprising all three of the amino acid sequences encoding large, medium and small subunits, selected from the group consisting of: a) SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12; b) SEQ ID NO:93, SEQ ID NO:95, and SEQ ID NO:97; c) SEQ ID NO:99, SEQ ID NO:101, and SEQ ID NO:103; d) SEQ ID NO:105, SEQ ID NO:107, and SEQ ID NO:109; e) SEQ ID NO:135, SEQ ID NO:136, and SEQ ID NO:137; f) SEQ ID NO:138, SEQ ID NO:139, and SEQ ID NO:140; g) SEQ ID NO:146, SEQ ID NO:148, and SEQ ID NO:150; h) SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:143; and i) SEQ ID NO:164, SEQ ID NO:165, and SEQ ID NO:166; based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.
 20. (canceled)
 21. A method for the production of 2-butanol comprising: 1) providing the recombinant microbial host cell of claim 1 and 2) contacting the host cell of (1) with a fermentable carbon substrate in a fermentation medium under conditions whereby 2-butanol is produced.
 22. A method for the production of 2-butanone comprising: 1) providing a recombinant microbial host cell of claim 2 and 2) contacting the host cell of (1) with a fermentable carbon substrate in a fermentation medium under conditions whereby 2-butanone is produced.
 23. A method according to claims 21 or 22 wherein the fermentable carbon substrate is selected from the group consisting of monosaccharides, oligosaccharides, and polysaccharides. 24-28. (canceled)
 29. A method according to claims 21 or 22 wherein the cell is selected from the group consisting of: a bacterium, a cyanobacterium, a filamentous fungus, and a yeast.
 30. A method according to claim 29 wherein the cell is a member of a genus selected from the group consisting of Clostridium, Zymomonas, Escherichia, Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Pediococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Pichia, Candida, Hansenula and Saccharomyces. 31-41. (canceled)
 42. A 2-butanol containing fermentation product medium produced by the method of claim
 21. 43. A 2-butanone containing fermentation product medium produced by the method of claim
 22. 